scholarly journals Polydopamine functionalized VEGF gene-activated 3D printed scaffolds for bone regeneration

RSC Advances ◽  
2021 ◽  
Vol 11 (22) ◽  
pp. 13282-13291
Author(s):  
Jaidev L. Chakka ◽  
Timothy Acri ◽  
Noah Z. Laird ◽  
Ling Zhong ◽  
Kyungsup Shin ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Blood Vessels ◽  
Bone Defects ◽  
Vegf Gene ◽  
3D Printed ◽  
Critical Bone Defects

Bone is a highly vascularized organ and the formation of new blood vessels is essential to regenerate large critical bone defects.

3D-printed Poly-Lactic Co-Glycolic Acid (PLGA) scaffolds in non-critical bone defects impede bone regeneration in rabbit tibia bone

2021 ◽  
pp. 1-7
Author(s):  
Jin Xi Lim ◽  
Min He ◽  
Alphonsus Khin Sze Chong
Keyword(s):  
Computed Tomography ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Volume Ratio ◽  
Bone Defects ◽  
Control Group ◽  
Micro Computed Tomography ◽  
Rabbit Tibia ◽  
3D Printed ◽  
Critical Bone Defects

BACKGROUND: An increasing number of bone graft materials are commercially available and vary in their composition, mechanism of action, costs, and indications. OBJECTIVE: A commercially available PLGA scaffold produced using 3D printing technology has been used to promote the preservation of the alveolar socket after tooth extraction. We examined its influence on bone regeneration in long bones of New Zealand White rabbits. METHODS: 5.0-mm-diameter circular defects were created on the tibia bones of eight rabbits. Two groups were studied: (1) control group, in which the bone defects were left empty; (2) scaffold group, in which the PLGA scaffolds were implanted into the bone defect. Radiography was performed every two weeks postoperatively. After sacrifice, bone specimens were isolated and examined by micro-computed tomography and histology. RESULTS: Scaffolds were not degraded by eight weeks after surgery. Micro-computed tomography and histology showed that in the region of bone defects that was occupied by scaffolds, bone regeneration was compromised and the total bone volume/total volume ratio (BV/TV) was significantly lower. CONCLUSION: The implantation of this scaffold impedes bone regeneration in a non-critical bone defect. Implantation of bone scaffolds, if unnecessary, lead to a slower rate of fracture healing.

scholarly journals Repair of critical-size porcine craniofacial bone defects using a collagen-polycaprolactone composite biomaterial

2021 ◽  
Author(s):  
Marley J Dewey ◽  
Derek J Milner ◽  
Daniel Weisgerber ◽  
Colleen Flanagan ◽  
Marcello Rubessa ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Fibrous Tissue ◽  
Bone Defects ◽  
Large Animal ◽  
Collagen Scaffolds ◽  
Shape Fitting ◽  
Large Animal Models ◽  
3D Printed ◽  
Mineralized Collagen

Regenerative medicine approaches for massive craniomaxillofacial bone defects face challenges associated with the scale of missing bone, the need for rapid graft-defect integration, and challenges related to inflammation and infection. Mineralized collagen scaffolds have been shown to promote mesenchymal stem cell osteogenesis due to their porous nature and material properties, but are mechanically weak, limiting surgical practicality. Previously, these scaffolds were combined with 3D-printed polycaprolactone mesh to form a scaffold-mesh composite to increase strength and promote bone formation in sub-critical sized porcine ramus defects. Here, we compare the performance of mineralized collagen-polycaprolactone composites to the polycaprolactone mesh in a critical-sized porcine ramus defect model. While there were no differences in overall healing response between groups, our data demonstrated broadly variable metrics of healing regarding new bone infiltration and fibrous tissue formation. Abscesses were present surrounding some implants and polycaprolactone polymer was still present after 9-10 months of implantation. Overall, while there was limited successful healing, with 2 of 22 implants showed substantial levels of bone regeneration, and others demonstrating some form of new bone formation, the results suggest targeted improvements to improve repair of large animal models to more accurately represent craniomaxillofacial bone healing. Notably, strategies to increase osteogenesis throughout the implant, modulate the immune system to support repair, and employ shape-fitting tactics to avoid implant micromotion and resultant fibrosis. Improvements to the mineralized collagen scaffolds involve changes in pore size and shape to increase cell migration and osteogenesis and inclusion or delivery of factors to aid vascular ingrowth and bone regeneration.

In vivo experimental study on bone regeneration in critical bone defects using an injectable biodegradable PLA/PGA copolymer

2005 ◽  
Vol 99 (2) ◽  
pp. 148-154 ◽  
Author(s):  
Lia Rimondini ◽  
Nicolò Nicoli-Aldini ◽  
Milena Fini ◽  
Gaetano Guzzardella ◽  
Matilde Tschon ◽  
...  
Keyword(s):  
Experimental Study ◽  
Bone Regeneration ◽  
Bone Defects ◽  
Critical Bone Defects

scholarly journals Improved Bone Regeneration in Rabbit Bone Defects Using 3D Printed Composite Scaffolds Functionalized with Osteoinductive Factors

2020 ◽  
Vol 12 (43) ◽  
pp. 48340-48356
Author(s):  
Arun Kumar Teotia ◽  
Kasper Dienel ◽  
Irfan Qayoom ◽  
Bas van Bochove ◽  
Sneha Gupta ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Defects ◽  
Composite Scaffolds ◽  
3D Printed ◽  
Rabbit Bone

Strontium folate loaded biohybrid scaffolds seeded with dental pulp stem cells induce in vivo bone regeneration in critical sized defects

2016 ◽  
Vol 4 (11) ◽  
pp. 1596-1604 ◽  
Author(s):  
Marcela Martin-del-Campo ◽  
Raul Rosales-Ibañez ◽  
Keila Alvarado ◽  
Jose G. Sampedro ◽  
Christian A. Garcia-Sepulveda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Dental Pulp ◽  
Bone Defects ◽  
Dental Pulp Stem Cells ◽  
Critical Bone Defects ◽  
Complete Regeneration

Strontium folate loaded biohybrid scaffolds enhance dental pulp stem cells replication and differentiation, promoting complete regeneration of critical bone defects.

scholarly journals In Vivo Investigation of Polymer-Ceramic PCL/HA and PCL/β-TCP 3D Composite Scaffolds and Electrical Stimulation for Bone Regeneration

Polymers ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 65
Author(s):  
Júlia Venturini Helaehil ◽  
Carina Basqueira Lourenço ◽  
Boyang Huang ◽  
Luiza Venturini Helaehil ◽  
Isaque Xavier de Camargo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Electrical Stimulation ◽  
Bone Regeneration ◽  
Bone Defects ◽  
Composite Scaffolds ◽  
Electrical Therapy ◽  
Biological Recognition ◽  
3D Composite ◽  
Critical Bone Defects

Critical bone defects are a major clinical challenge in reconstructive bone surgery. Polycaprolactone (PCL) mixed with bioceramics, such as hydroxyapatite (HA) and tricalcium phosphate (TCP), create composite scaffolds with improved biological recognition and bioactivity. Electrical stimulation (ES) aims to compensate the compromised endogenous electrical signals and to stimulate cell proliferation and differentiation. We investigated the effects of composite scaffolds (PCL with HA; and PCL with β-TCP) and the use of ES on critical bone defects in Wistar rats using eight experimental groups: untreated, ES, PCL, PCL/ES, HA, HA/ES, TCP, and TCP/ES. The investigation was based on histomorphometry, immunohistochemistry, and gene expression analysis. The vascular area was greater in the HA/ES group on days 30 and 60. Tissue mineralization was greater in the HA, HA/ES, and TCP groups at day 30, and TCP/ES at day 60. Bmp-2 gene expression was higher in the HA, TCP, and TCP/ES groups at day 30, and in the TCP/ES and PCL/ES groups at day 60. Runx-2, Osterix, and Osteopontin gene expression were also higher in the TCP/ES group at day 60. These results suggest that scaffolds printed with PCL and TCP, when paired with electrical therapy application, improve bone regeneration.

scholarly journals 3D-printed HA15-loaded β-Tricalcium Phosphate/Poly (Lactic-co-glycolic acid) Bone Tissue Scaffold Promotes Bone Regeneration in Rabbit Radial Defects

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Chuanchuan Zheng ◽  
Shokouh Attarilar ◽  
Kai Li ◽  
Chong Wang ◽  
Jia Liu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Glycolic Acid ◽  
Bone Conduction ◽  
Biomechanical Properties ◽  
Bone Defects ◽  
Tissue Scaffold ◽  
3D Printed

In this study, a β-tricalcium phosphate (β-TCP)/poly (lactic-co-glycolic acid) (PLGA) bone tissue scaffold was loaded with osteogenesis-promoting drug HA15 and constructed by three-dimensional (3D) printing technology. This drug delivery system with favorable biomechanical properties, bone conduction function, and local release of osteogenic drugs could provide the basis for the treatment of bone defects. The biomechanical properties of the scaffold were investigated by compressive testing, showing comparable biomechanical properties with cancellous bone tissue. Furthermore, the microstructure, pore morphology, and condition were studied. Moreover, the drug release concentration, the effect of anti-tuberculosis drugs in vitro and in rabbit radial defects, and the ability of the scaffold to repair the defects were studied. The results show that the scaffold loaded with HA15 can promote cell differentiation into osteoblasts in vitro, targeting HSPA5. The micro-computed tomography scans showed that after 12 weeks of scaffold implantation, the defect of the rabbit radius was repaired and the peripheral blood vessels were regenerated. Thus, HA15 can target HSPA5 to inhibit endoplasmic reticulum stress which finally leads to promotion of osteogenesis, bone regeneration, and angiogenesis in the rabbit bone defect model. Overall, the 3D-printed β-TCP/PLGA-loaded HA15 bone tissue scaffold can be used as a substitute material for the treatment of bone defects because of its unique biomechanical properties and bone conductivity.

Thermal Stress Conditioning to Induce Osteogenic Protein Expression for Bone Regeneration

2012 ◽  
Author(s):  
Alana C. Sampson ◽  
Eunna Chung ◽  
Marissa Nichole Rylander
Keyword(s):  
Bone Regeneration ◽  
Bone Growth ◽  
Bone Defects ◽  
Regenerative Capacity ◽  
Wound Site ◽  
Essential Proteins ◽  
Osteogenic Protein ◽  
Exogenous Delivery ◽  
Critical Bone Defects ◽  
External Stimuli

Although bone has the intrinsic ability to “self-heal”, there are circumstances in which its regenerative capacity is limited or compromised, such as in critical bone defects. In these cases, the lack of osteogenic proteins at the wound site can prevent healing and external stimuli may be necessary to encourage bone growth [1]. Exogenous delivery of proteins and growth factors directly to the wound has been successful in bone regeneration, but is limited by the instability of the proteins and short half-lives. As a result, administration of multiple large doses of protein is necessary to retain a beneficial protein level. Due to these disadvantages, additional methods have been investigated to supply essential proteins to the bone defect [2].

scholarly journals In vivo study of conductive 3D printed PCL/MWCNTs scaffolds with electrical stimulation for bone tissue engineering

2021 ◽  
Author(s):  
Edney P. e Silva ◽  
Boyang Huang ◽  
Júlia V. Helaehil ◽  
Paulo R. L. Nalesso ◽  
Leonardo Bagne ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Defects ◽  
Tartrate Resistant Acid Phosphatase ◽  
High Concentration ◽  
3D Printed ◽  
Critical Bone Defects

AbstractCritical bone defects are considered one of the major clinical challenges in reconstructive bone surgery. The combination of 3D printed conductive scaffolds and exogenous electrical stimulation (ES) is a potential favorable approach for bone tissue repair. In this study, 3D conductive scaffolds made with biocompatible and biodegradable polycaprolactone (PCL) and multi-walled carbon nanotubes (MWCNTs) were produced using the extrusion-based additive manufacturing to treat large calvary bone defects in rats. Histology results show that the use of PCL/MWCNTs scaffolds and ES contributes to thicker and increased bone tissue formation within the bone defect. Angiogenesis and mineralization are also significantly promoted using high concentration of MWCNTs (3 wt%) and ES. Moreover, scaffolds favor the tartrate-resistant acid phosphatase (TRAP) positive cell formation, while the addition of MWCNTs seems to inhibit the osteoclastogenesis but present limited effects on the osteoclast functionalities (receptor activator of nuclear factor κβ ligand (RANKL) and osteoprotegerin (OPG) expressions). The use of ES promotes the osteoclastogenesis and RANKL expressions, showing a dominant effect in the bone remodeling process. These results indicate that the combination of 3D printed conductive PCL/MWCNTs scaffold and ES is a promising strategy to treat critical bone defects and provide a cue to establish an optimal protocol to use conductive scaffolds and ES for bone tissue engineering.

Cryogel scaffolds from patient-specific 3D-printed molds for personalized tissue-engineered bone regeneration in pediatric cleft-craniofacial defects

2017 ◽  
Vol 32 (5) ◽  
pp. 598-611 ◽  
Author(s):  
Katherine R Hixon ◽  
Alexa M Melvin ◽  
Alexander Y Lin ◽  
Andrew F Hall ◽  
Scott A Sell
Keyword(s):  
Bone Regeneration ◽  
Bone Defects ◽  
Patient Specific ◽  
Proof Of Concept ◽  
Site Specific ◽  
Alveolar Cleft ◽  
Mechanical Durability ◽  
Specific Geometry ◽  
3D Printed ◽  
Craniofacial Defects

Bone defects are extremely common in children with cleft-craniofacial conditions, especially those with alveolar cleft defects and cranial defects. This study used patient-specific 3D-printed molds derived from computed tomography and cryogel scaffold fabrication as a proof of concept for the creation of site-specific implants for bone reconstruction. Cryogel scaffolds are unique tissue-engineered constructs formed at sub-zero temperatures. When thawed, the resulting structure is macroporous, sponge-like, and mechanically durable. Due to these unique properties, cryogels have excellent potential for the treatment of patient-specific bone defects; however, there is little literature on their use in cleft-craniofacial defects. While 3D-printing technology currently lacks the spatial resolution to print the microstructure necessary for bone regeneration, it can be used to create site-specific molds. Thus, it is ideal to integrate these techniques for the fabrication of scaffolds with patient-specific geometry. Overall, all cryogels possessed appropriate geometry to allow for cell infiltration after 28 days. Additionally, suitable mechanical durability was demonstrated where, despite mold geometry, all cryogels could be compressed without exhibiting crack propagation. Such a patient-specific scaffold would be ideal in pediatric cleft-craniofacial defects, as these are complex 3D defects and children have less donor bone availability.

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