scholarly journals Ethyl glucuronide, a marker of alcohol consumption, correlates with metabolic markers of oxidant stress but not with hemolysis in stored red blood cells from healthy blood donors

Transfusion ◽  
2020 ◽  
Vol 60 (6) ◽  
pp. 1183-1196 ◽  
Author(s):  
Angelo D'Alessandro ◽  
Xiaoyun Fu ◽  
Julie A. Reisz ◽  
Mars Stone ◽  
Steve Kleinman ◽  
...  
Keyword(s):  
Alcohol Consumption ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Donors ◽  
Oxidant Stress ◽  
Ethyl Glucuronide ◽  
Metabolic Markers

Deformability and Oxidant Stress in Red Blood Cells under the Influence of Halothane and Isoflurane Anesthesia

1998 ◽  
Vol 31 (1) ◽  
pp. 33-36 ◽  
Author(s):  
Akin Yesilkaya ◽  
Zeki Ertug ◽  
Yegin ◽  
Mustafa Melikoglu ◽  
Oguz K. Baskurt
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Oxidant Stress ◽  
Isoflurane Anesthesia

scholarly journals Testing for Partial RhD with a D-Screen Diagast Kit in Moroccan Blood Donors with Weak D Expression

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Z. Kabiri ◽  
M. Benajiba ◽  
K. Hajjout ◽  
N. Dakka ◽  
H. Bellaoui
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Donors ◽  
Formal Argument ◽  
Appropriate Care ◽  
Specific Reactions ◽  
D Antigen

The aim of this study was to search for the partial D phenotype in Moroccan blood donors with weak D expression. The study included 32 samples with weak D phenotype, and partial D category red blood cells were detected with the D-Screen Diagast kit, which consists in 9 monoclonal anti-D antibodies specific for the most common categories of partial D. Among the 32 samples studied, we identified 13 specific reactions to a partial D antigen (3 DVI, 2 DVa, 2 DIII(a,b,c), and 6 DVII), with 8 reactions suggesting a weak D and 11 reactions providing no formal argument in favor of a partial D antigen. This work can be used to validate the performance of the anti-D reagent and to improve the safety of transfusion of red blood cells from donors expressing the partial D antigen by integrating the finding into the recipient file with a recommendation concerning the appropriate care.

scholarly journals Condition of erythrocyte membranes in people with mental and behavioural disorders caused by alcohol consumption

2017 ◽  
Vol 8 (2) ◽  
pp. 111-117
Author(s):  
O. V. Baskevich
Keyword(s):  
Alcohol Consumption ◽  
Mental Disorders ◽  
Red Blood Cells ◽  
Blood Cells ◽  
The State ◽  
Biologically Active ◽  
Erythrocyte Membranes ◽  
Osmotic Resistance ◽  
Behavioural Disorders ◽  
Body Tissues

We studied the morphological features of the restructuring and osmotic stability of erythrocytes of 30 people with mental disorders and disorders of behavioural reactions caused by 5-7 years consumption of alcohol, the objects of the research being in a state of abstinence during the period of the investigation,. The study of quantitative composition of red blood cells and hemoglobin, as well as their ratio in people with mental and behavioural disorders developed as a result of alcohol consumption showed a decrease in the number of red blood cells and hemoglobin of 42.2% and 25.1%, respectively, indicating the presence in those people of anemic hypoxia. This is closely correlated with a decrease of osmotic resistance of red blood cells to different concentrations of sodium chloride solutions, whereby the state of erythrocyte membranes deteriorates and their resistance to hemolysis decreases. Parallel to this, is a reduction in the size of red blood cells coupled with a variety of changes in their microrelief. Conformational changes of erythrocytes are closely correlated with changes in the activity of enzymes responsible for the biotransformation of xenobiotics, primarily products of lipid peroxidation. It is shown that activity of the erythrocyte antioxidant defense system is sharply reduced as a result of increase in partially oxidized products of metabolism. It was found that the state of red blood cells is a sensitive indicator of the normal course of physiological changes, biochemical and biophysical processes in the bodies of people with mental disorders and behaviour reactions developed due to consumption of alcohol.At the same time, the measurement of the osmotic resistance of red blood cells and changes in the shape of red blood cells connected with this phenomenon is an important research method for studying mechanisms of pathological processes and the action of certain drugs or biologically active substances, expands the possibilities of establishing the depth of destruction of body tissues, and also provides excellent opportunities for researchers to identify the effectiveness of treatment and prevention for people with mental disorders and behaviour reactions caused by consumption of alcohol. This article discusses prospects of studies of the level of glutathione peroxidase activity in the erythrocytes among people with mental and behavioural disorders as a marker of highly specific homeostasis in the human body in response to long-term alcohol consumption, and considers using this index as a criterion for selecting patients for different groups of physical rehabilitation and recreation according to the level of rehabilitation potential.

Cell-Derived Microparticles in Blood Products from Thalassemic Blood Donors

2020 ◽  
Author(s):  
Egarit Noulsri ◽  
Surada Lerdwana ◽  
Duangdao Palasuwan ◽  
Attakorn Palasuwan
Keyword(s):  
Flow Cytometry ◽  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Blood Donors ◽  
Blood Components ◽  
Blood Products ◽  
Packed Red Blood Cells ◽  
Thalassemia Trait ◽  
Routine Procedures

Abstract Objective To determine the number of cell-derived microparticles (MPs) in blood products obtained from donors who have thalassemia. Methods Packed red blood cells (PRBCs), plasma, and platelet concentrate (PC) were prepared according to routine procedures. We used flow cytometry to quantitate the concentration of MPs. Results The results of a comparison of MP levels in unprocessed whole blood showed that the concentration of all MPs in the donors without thalassemia trait (n = 255) was higher than in donors with thalassemia trait (n = 70). After processing, increased concentrations of MPs were documented in both groups. Among the blood components, PRBC showed higher platelet-derived MP concentrations in donors with thalassemia than in donors without thalassemia. However, PC showed higher concentrations of total MPs in donors without thalassemia than in donors with that condition. Conclusions Our results suggest little influence of thalassemia-trait status on changes in MP concentrations in blood components.

Indicators of red blood in donors working in enterprises with harmful production

2020 ◽  
pp. 48-52
Author(s):  
A. V. Elifanov ◽  
O. N. Lepunova ◽  
O. V. Frolova ◽  
O. L. Kovyazina ◽  
A. D. Shalabodov
Keyword(s):  
Red Blood Cells ◽  
Working Conditions ◽  
Blood Cells ◽  
Blood Donors ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Blood System ◽  
Conflict Of Interests ◽  
Subjective Assessments ◽  
Gender And Age

The study of the movement of donation in enterprises with harmful working conditions in Tyumen. The influence of professional factors on red blood parameters (hemoglobin concentration, number of red blood cells, ESR, hematocrit, color index) of donors with regard to gender and age was studied. According to the results of a survey of 4,267 blood donors in 2013–2016, there was an increase in the number of withdrawals from donations, due to changes in red blood indicators, in workers at enterprises with harmful working conditions. The examined men and women, whose work is associated with harmful occupational factors, showed a significant decrease in the concentration of hemoglobin and the number of red blood cells. Objective hematological observations along with subjective assessments of the donors ‘own health showed the development of anemic syndrome — one of the main syndromes of lead professional pathology. It is necessary to continue searching for factors that cause an increase in the percentage of donor withdrawal due to low hemoglobin concentrations. Among the promising measures for maintaining and developing donation in enterprises with harmful working conditions, specific plans for improving health should be developed, including specific tools and conditions for improving the blood system.Funding. The study had no funding.Conflict of interests. The authors declare no conflict of interests.

Sign in / Sign up

Export Citation Format

Share Document