scholarly journals Testing for Partial RhD with a D-Screen Diagast Kit in Moroccan Blood Donors with Weak D Expression

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Z. Kabiri ◽  
M. Benajiba ◽  
K. Hajjout ◽  
N. Dakka ◽  
H. Bellaoui
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Donors ◽  
Formal Argument ◽  
Appropriate Care ◽  
Specific Reactions ◽  
D Antigen

The aim of this study was to search for the partial D phenotype in Moroccan blood donors with weak D expression. The study included 32 samples with weak D phenotype, and partial D category red blood cells were detected with the D-Screen Diagast kit, which consists in 9 monoclonal anti-D antibodies specific for the most common categories of partial D. Among the 32 samples studied, we identified 13 specific reactions to a partial D antigen (3 DVI, 2 DVa, 2 DIII(a,b,c), and 6 DVII), with 8 reactions suggesting a weak D and 11 reactions providing no formal argument in favor of a partial D antigen. This work can be used to validate the performance of the anti-D reagent and to improve the safety of transfusion of red blood cells from donors expressing the partial D antigen by integrating the finding into the recipient file with a recommendation concerning the appropriate care.

scholarly journals Improvement of bovine tuberculosis diagnosis

2021 ◽  
pp. 261-265
Author(s):  
M. O. Baratov
Keyword(s):  
Red Blood Cells ◽  
Mycobacterium Bovis ◽  
Blood Cells ◽  
Bovine Tuberculosis ◽  
Intradermal Test ◽  
Mycobacterium Fortuitum ◽  
Post Mortem ◽  
Post Mortem Examination ◽  
Antibody Titers ◽  
Specific Reactions

Detection of animals with non-specific reactions to tuberculin is one of the major problems in bovine tuberculosis (TB) diagnosis. There is a need to find and improve methods for detection of the sensitization causes. This paper presents the results of comparative studies of different ways to stabilize red blood cells in order to obtain diagnosticums for indirect hemagglutination (IHA) test. The article describes the stages of red blood cells stabilization and sensitization and demonstrates the diagnostic significance of Fili stabilization method using formaldehyde as a fixative. The highest antibody titers (1:3000 and 1:4000) were received in hyperimmune sera of rabbits immunized with Mycobacterium bovis using a homologous diagnosticum. Practical importance of the sensitins homologous to the infection is shown during testing of 1,911 serum samples collected from animals of different categories (diseased; healthy and reacting to tuberculin; healthy and not reacting to tuberculin) with IHA test using diagnosticums produced from Mycobacterium bovis and Mycobacterium fortuitum. Based on the positive results of the IHA test, TB was diagnosed in 87.5% of animals originating from an infected farm during post-mortem examination. The results of the IHA test agreed with those of the intradermal tuberculin test in 37.7% of cases. Diagnostic antibody titers were found in 206 TB infected animals with no reaction to the intradermal test. However, the post-mortem examination revealed TB changes in internal organs. The obtained data suggest a possibility to use the IHA test to detect TB infected animals with non-specific reactions to tuberculin.

scholarly journals Loss and reappearance of RhO(D) antigen on the red blood cells of an individual with acute myelogenous leukemia

2020 ◽  
Vol 10 (4) ◽  
pp. 134-135
Author(s):  
Kala Mohandas ◽  
Vesna Najfeld ◽  
Harriet Gilbert ◽  
Penny Azar ◽  
Donna Skerrett
Keyword(s):  
Red Blood Cells ◽  
Acute Myelogenous Leukemia ◽  
Blood Cells ◽  
Myelogenous Leukemia ◽  
Acute Myelogenous ◽  
D Antigen

scholarly journals Ethyl glucuronide, a marker of alcohol consumption, correlates with metabolic markers of oxidant stress but not with hemolysis in stored red blood cells from healthy blood donors

Transfusion ◽  
2020 ◽  
Vol 60 (6) ◽  
pp. 1183-1196 ◽  
Author(s):  
Angelo D'Alessandro ◽  
Xiaoyun Fu ◽  
Julie A. Reisz ◽  
Mars Stone ◽  
Steve Kleinman ◽  
...  
Keyword(s):  
Alcohol Consumption ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Donors ◽  
Oxidant Stress ◽  
Ethyl Glucuronide ◽  
Metabolic Markers

scholarly journals Blood group D antigen content of nucleated red cell precursors

Blood ◽  
1977 ◽  
Vol 50 (6) ◽  
pp. 981-986 ◽  
Author(s):  
A Rearden ◽  
SP Masouredis
Keyword(s):  
Bone Marrow ◽  
Red Blood Cells ◽  
Blood Group ◽  
Blood Cells ◽  
Cell Types ◽  
The Other ◽  
Cell Maturation ◽  
Red Cell ◽  
D Antigen ◽  
Group D

Abstract The D antigen content of nucleated red cell precursors in human bone marrow was estimated using autoradiography and 125I-anti-D. D antigen first appeared in the pronormoblast, and the quantity of antigen progressively increased during red cell maturation. Maximal anti-D binding occurred on mature red blood cells. Pronormoblasts, basophilic normoblasts, polychromatophilic normoblasts, and orthochromatic normoblasts, respectively, had approximately 1/4, 1/2, 2/3, and 3/4 the quantity of antigen found on mature red cells. None of the other cell types were found in bone marrow labeled with anti-D.

scholarly journals Indirect hemagglutination reaction in the differential diagnosis of bovine tuberculosis

2021 ◽  
Author(s):  
M.O. Baratov ◽  
O.P. Sakidibirov ◽  
P.S. Huseynova
Keyword(s):  
Differential Diagnosis ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Molecular Genetic ◽  
Tuberculin Test ◽  
Diagnostic Methods ◽  
Practical Significance ◽  
Mycobacterium Fortuitum ◽  
Indirect Hemagglutination ◽  
Specific Reactions

The main problem of improving the health of farms from tuberculosis is diagnosis, in particular, differential diagnosis. Despite the comprehensive study and a fairly impressive number of proposed diagnostic methods, including on the molecular genetic level, the problem of differentiation of non-specific reactions remains topical today. About effectiveness of serological methods of research, in particular, indirect hemagglutination reactions, in the literature there are scattered and polar data. In the methods of setting up reactions, in the variety of antigens, methods of adsorption of agglutinins, stabilization of red blood cells, etc., the researchers ' opinions differ. Four methods of stabilization of sheep erythrocytes sensitized with polysaccharide antigen (Feeli, Weinbach, Ling, RES) have been comparatively studied. The maximum antibody titer was established for the stabilization of red blood cells by Feeli. A total of 1911 blood samples from four farms with different relationships to tuberculosis were studied in the reaction of indirect hemagglutination with diagnostics from Mycobacterium bovis and Mycobacterium fortuitum. Relatively high positive indicators of the reaction of indirect hemagglutination were revealed in animals in farms with tuberculosis problems, in which the diagnosis was confirmed by pathoanatomic method in 87.5% of cases. The results of the tuberculin test coincided with the results of indirect hemagglutination reactions in 37.07% of cases. Diagnostic antibody titers were detected in 201 anergic animals of the treated herd and in 5 from the isolation unit. During the slaughter of 20 heads from a dysfunctional farm and 5 from the overexposure group, tuberculosis was established in 15 and 5, respectively. The practical significance of the reaction of indirect hemagglutination with different diagnostics in the detection of homologously infected animals is shown.

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