scholarly journals A novel multiple membrane blood-feeding system for investigating and maintaining Aedes aegypti and Aedes albopictus mosquitoes

2014 ◽  
Vol 39 (2) ◽  
pp. 271-277 ◽  
Author(s):  
Yi-Pey Luo
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Blood Feeding ◽  
Feeding System

scholarly journals Comparative Analyses on Synthetic Membranes for Artificial Blood Feeding of Aedes aegypti using Digital Thermo Mosquito Blood Feeder (DITMOF)

2021 ◽  
Vol 29 (3) ◽  
Author(s):  
Mohd Farihan Md Yatim ◽  
Aishah Hani Azil ◽  
Nazarudin Safian ◽  
Ahmad Firdaus Mohd Salleh ◽  
Mohd Khadri Shahar
Keyword(s):  
Aedes Aegypti ◽  
Feeding Rate ◽  
Mosquito Species ◽  
Blood Feeding ◽  
Feeding System ◽  
Live Animal ◽  
Synthetic Membranes ◽  
Artificial Blood ◽  
Cattle Blood ◽  
Sausage Casing

The use of live animal to blood feeding mosquito colony is proven to be expensive and inconvenient. As an alternative, artificial feeding (AF) is used to rear mosquito colony. The use of synthetic membrane in AF provided a more convenient method as compared to natural membrane which require extensive preparation. In this study, three synthetic membranes were compared (Parafilm-M, Polytetrafluoroethylene tape or PTFE tape and collagen sausage casing) to blood feeding Aedes aegypti. The membranes were incorporated with our in-house developed device named as Digital Thermo Mosquito Blood Feeder (DITMOF) to heat cattle blood for mosquito feeding. Results showed that PTFE tape recorded the highest blood feeding rate (95.00% ± 1.67%) with significant mean difference (p <0.001) as compared to both Parafilm-M (72.00% ± 2.60%) and collagen sausage casing (71.50% ± 3.50%). However, there was no difference in term of fecundity for mosquito feed with all three membranes tested (p=0.292). In conclusion, PTFE tape should be considered as the preferred membrane to blood feeding Ae. aegypti. Furthermore, this artificial blood feeding system, DITMOF successfully feed Ae. aegypti conveniently and effectively, thus should be further tested to feed other mosquito species.

scholarly journals Nootkatone Is an Effective Repellent against Aedes aegypti and Aedes albopictus

Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 386
Author(s):  
Taylor C. Clarkson ◽  
Ashley J. Janich ◽  
Irma Sanchez-Vargas ◽  
Erin D. Markle ◽  
Megan Gray ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Insecticide Resistance ◽  
New Orleans ◽  
Aedes Albopictus ◽  
Zika Virus ◽  
Activity Index ◽  
Human Subjects ◽  
Blood Feeding ◽  
Spatial Activity ◽  
Insecticide Activity

We tested a nootkatone product for insecticide activity against the most prominent vectors of Zika virus (ZIKV), Aedes aegypti, and Aedes albopictus. We tested the permethrin-resistant (PERM-R) Vergel strain of A. aegypti and the permethrin-susceptible (PERM-S) New Orleans strain of A. aegypti to determine if insecticide resistance affected their susceptibility to nootkatone. Bottle bioassays showed that the PERM-S strain (New Orleans) was more susceptible to nootkatone than the confirmed A. aegypti permethrin-resistant (PERM-R) strain, Vergel. The A. albopictus strain ATM-NJ95 was a known PERM-S strain and Coatzacoalcos permethrin susceptibility was unknown but proved to be similar to the ATM-NJ95 PERM-S phenotype. The A. albopictus strains (ATM-NJ95 and Coatzacoalcos) were as susceptible to nootkatone as the New Orleans strain. Bottle bioassays conducted with ZIKV-infected mosquitoes showed that the New Orleans (PERM-S) strain was as susceptible to nootkatone as the mock-infected controls, but the PERM-R strain was less susceptible to nootkatone than the mock-infected controls. Repellency/irritancy and biting inhibition bioassays (RIBB) of A. aegypti determined whether the nootkatone-treated arms of three human subjects prevented uninfected A. aegypti mosquitoes from being attracted to the test subjects and blood-feeding on them. The RIBB analyses data calculated the spatial activity index (SAI) and biting inhibition factor (BI) of A. aegypti at different nootkatone concentrations and then compared the SAI and BI of existing repellency products. We concluded that nootkatone repelled mosquitoes at a rate comparable to 7% DEET or 5% picaridin and has the potential to be an efficacious repellent against adult A. aegypti mosquitoes.

scholarly journals Evaluation of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus Mosquitoes Competence to Oropouche virus Infection

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 755
Author(s):  
Silvana F. de Mendonça ◽  
Marcele N. Rocha ◽  
Flávia V. Ferreira ◽  
Thiago H. J. F Leite ◽  
Siad C. G. Amadou ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Culex Quinquefasciatus ◽  
Mosquito Species ◽  
Blood Feeding ◽  
Viral Pathogens ◽  
Tropical Regions ◽  
Vertebrate Hosts ◽  
Geographic Expansion ◽  
Immunocompromised Mice

The emergence of new human viral pathogens and re-emergence of several diseases are of particular concern in the last decades. Oropouche orthobunyavirus (OROV) is an arbovirus endemic to South and Central America tropical regions, responsible to several epidemic events in the last decades. There is little information regarding the ability of OROV to be transmitted by urban/peri-urban mosquitoes, which has limited the predictability of the emergence of permanent urban transmission cycles. Here, we evaluated the ability of OROV to infect, replicate, and be transmitted by three anthropophilic and urban species of mosquitoes, Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus. We show that OROV is able to infect and efficiently replicate when systemically injected in all three species tested, but not when orally ingested. Moreover, we find that, once OROV replication has occurred in the mosquito body, all three species were able to transmit the virus to immunocompromised mice during blood feeding. These data provide evidence that OROV is restricted by the midgut barrier of three major urban mosquito species, but, if this restriction is overcome, could be efficiently transmitted to vertebrate hosts. This poses a great risk for the emergence of permanent urban cycles and geographic expansion of OROV to other continents.

scholarly journals Increased temperatures reduce the vectorial capacity of Aedes mosquitoes for Zika virus

2019 ◽  
Author(s):  
Onyango Maria Gorreti ◽  
Bialosuknia Sean ◽  
Payne Ann ◽  
Mathias Nicholas ◽  
Kuo Lilli ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Vector Competence ◽  
Blood Feeding ◽  
Transmission Efficiency ◽  
Vectorial Capacity ◽  
Future Climate Change ◽  
Feeding Rates ◽  
Chi Square ◽  
Transmission Potential

AbstractRapid and significant range expansion of both ZIKV and its Aedes host species has resulted in ZIKV being declared a global health threat. Mean temperatures are projected to increase globally, likely resulting in alterations of the transmission potential of mosquito-borne pathogens. The relationship between temperature and ZIKV transmission has not been well characterised for Aedes aegypti and Aedes albopictus.To understand the effect of diurnal temperature range on the vectorial capacity of Aedes aegypti and Aedes albopictus for ZIKV, factors contributing to transmission potential were measured at different temperature regimens. Their longevity and blood feeding rates were assessed, and vector competence was determined following feeding on blood meals with 8.3 log10 PFU/ml ZIKV.Higher temperature resulted in decreased longevity of Ae. aegypti [Log-rank (Mantel-Cox) Test, Chi-square, df 35.66, 5 P (<0.0001)] and a significant decrease in blood feeding rates across groups [Z score (−5.8478) P (0.0444)]. Temperature had a population and species-specific impact on ZIKV infection rates. Overall, Ae. albopictus reared at the lowest temperature regimen demonstrated the highest vectorial capacity (1.63) and the highest transmission efficiency (57%). Temperature increases decreased vectorial capacity across groups, yet the largest decreases were measured for Ae. aegypti.The results of this study suggest that future climate change could significantly impact vector competence, blood feeding behavior and longevity, and therefore decrease the overall vectorial capacity of Aedes mosquitoes. It is also clear that this impact is likely to be both species and population-specific.

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