scholarly journals Effect of body size on multiple blood feeding and egg retention of Aedes aegypti (L.) and Aedes albopictus (Skuse) (Diptera: Culicidae)

2012 ◽  
Vol 63 (2) ◽  
pp. 123-131 ◽  
Author(s):  
Thahsin FARJANA ◽  
Nobuko TUNO
Keyword(s):  
Body Size ◽  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Blood Feeding ◽  
Egg Retention ◽  
Multiple Blood

Detection of Multiple Blood Feeding in Aedes aegypti (Diptera: Culicidae) During a Single Gonotrophic Cycle Using a Histologic Technique

1993 ◽  
Vol 30 (1) ◽  
pp. 94-99 ◽  
Author(s):  
Thomas W. Scott ◽  
Gary G. Clark ◽  
Leslie H. Lorenz ◽  
Priyanie H. Amerasinghe ◽  
Paul Reiter ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Blood Feeding ◽  
Gonotrophic Cycle ◽  
Multiple Blood ◽  
Histologic Technique

scholarly journals Nootkatone Is an Effective Repellent against Aedes aegypti and Aedes albopictus

Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 386
Author(s):  
Taylor C. Clarkson ◽  
Ashley J. Janich ◽  
Irma Sanchez-Vargas ◽  
Erin D. Markle ◽  
Megan Gray ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Insecticide Resistance ◽  
New Orleans ◽  
Aedes Albopictus ◽  
Zika Virus ◽  
Activity Index ◽  
Human Subjects ◽  
Blood Feeding ◽  
Spatial Activity ◽  
Insecticide Activity

We tested a nootkatone product for insecticide activity against the most prominent vectors of Zika virus (ZIKV), Aedes aegypti, and Aedes albopictus. We tested the permethrin-resistant (PERM-R) Vergel strain of A. aegypti and the permethrin-susceptible (PERM-S) New Orleans strain of A. aegypti to determine if insecticide resistance affected their susceptibility to nootkatone. Bottle bioassays showed that the PERM-S strain (New Orleans) was more susceptible to nootkatone than the confirmed A. aegypti permethrin-resistant (PERM-R) strain, Vergel. The A. albopictus strain ATM-NJ95 was a known PERM-S strain and Coatzacoalcos permethrin susceptibility was unknown but proved to be similar to the ATM-NJ95 PERM-S phenotype. The A. albopictus strains (ATM-NJ95 and Coatzacoalcos) were as susceptible to nootkatone as the New Orleans strain. Bottle bioassays conducted with ZIKV-infected mosquitoes showed that the New Orleans (PERM-S) strain was as susceptible to nootkatone as the mock-infected controls, but the PERM-R strain was less susceptible to nootkatone than the mock-infected controls. Repellency/irritancy and biting inhibition bioassays (RIBB) of A. aegypti determined whether the nootkatone-treated arms of three human subjects prevented uninfected A. aegypti mosquitoes from being attracted to the test subjects and blood-feeding on them. The RIBB analyses data calculated the spatial activity index (SAI) and biting inhibition factor (BI) of A. aegypti at different nootkatone concentrations and then compared the SAI and BI of existing repellency products. We concluded that nootkatone repelled mosquitoes at a rate comparable to 7% DEET or 5% picaridin and has the potential to be an efficacious repellent against adult A. aegypti mosquitoes.

scholarly journals Evaluation of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus Mosquitoes Competence to Oropouche virus Infection

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 755
Author(s):  
Silvana F. de Mendonça ◽  
Marcele N. Rocha ◽  
Flávia V. Ferreira ◽  
Thiago H. J. F Leite ◽  
Siad C. G. Amadou ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Culex Quinquefasciatus ◽  
Mosquito Species ◽  
Blood Feeding ◽  
Viral Pathogens ◽  
Tropical Regions ◽  
Vertebrate Hosts ◽  
Geographic Expansion ◽  
Immunocompromised Mice

The emergence of new human viral pathogens and re-emergence of several diseases are of particular concern in the last decades. Oropouche orthobunyavirus (OROV) is an arbovirus endemic to South and Central America tropical regions, responsible to several epidemic events in the last decades. There is little information regarding the ability of OROV to be transmitted by urban/peri-urban mosquitoes, which has limited the predictability of the emergence of permanent urban transmission cycles. Here, we evaluated the ability of OROV to infect, replicate, and be transmitted by three anthropophilic and urban species of mosquitoes, Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus. We show that OROV is able to infect and efficiently replicate when systemically injected in all three species tested, but not when orally ingested. Moreover, we find that, once OROV replication has occurred in the mosquito body, all three species were able to transmit the virus to immunocompromised mice during blood feeding. These data provide evidence that OROV is restricted by the midgut barrier of three major urban mosquito species, but, if this restriction is overcome, could be efficiently transmitted to vertebrate hosts. This poses a great risk for the emergence of permanent urban cycles and geographic expansion of OROV to other continents.

scholarly journals Serotonin signaling regulates insulin-like peptides for growth, reproduction, and metabolism in the disease vector Aedes aegypti

2018 ◽  
Vol 115 (42) ◽  
pp. E9822-E9831 ◽  
Author(s):  
Lin Ling ◽  
Alexander S. Raikhel
Keyword(s):  
Body Size ◽  
Aedes Aegypti ◽  
Serotonin Receptor ◽  
Fat Body ◽  
Transcript Level ◽  
Blood Feeding ◽  
Rnai Silencing ◽  
Regulatory Link ◽  
Serotonin Signaling ◽  
Lipid Stores

Disease-transmitting female mosquitoes require a vertebrate blood meal to produce their eggs. An obligatory hematophagous lifestyle, rapid reproduction, and existence of a large number of transmittable diseases make mosquitoes the world’s deadliest animals. Attaining optimal body size and nutritional status is critical for mosquitoes to become reproductively competent and effective disease vectors. We report that blood feeding boosts serotonin concentration and elevates the serotonin receptor Aa5HT2B (Aedes aegypti 5-hydroxytryptamine receptor, type 2B) transcript level in the fat-body, an insect analog of the vertebrate liver and adipose tissue. Aa5HT2B gene disruption using the CRISPR-Cas9 gene-editing approach led to a decreased body size, postponed development, shortened lifespan, retarded ovarian growth, and dramatically diminished lipid accumulation. Expression of the insulin-like peptide (ILP) genes ilp2 and ilp6 was down-regulated while that of ilp5 and ilp4 was up-regulated in response to Aa5HT2B disruption. CRISPR-Cas9 disruption of ilp2 or ilp6 resulted in adverse phenotypes similar to those of Aa5HT2B disruption, while ilp5 CRISPR-Cas9 disruption had exactly the opposite effect on growth and metabolism, with significantly increased body size and elevated lipid stores. Simultaneous CRISPR-Cas9 disruption of Aa5HT2B and ilp5 rescued these phenotypic manifestations. Aa5HT2B RNAi silencing rendered ilp6 insensitive to serotonin treatment in the cultured fat-body, suggesting a regulatory link between Aa5HT2B and ILP6. Moreover, CRISPR-Cas9 ilp6 disruption affects expression of ilp-2, -5, and -4, pointing out on a possible role of ILP6 as a mediator of the Aa5HT2B action.

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