High Resolution Proteome of Lipid Droplets Isolated from the Pennate Diatom Phaeodactylum tricornutum ( Bacillariophyceae ) Strain pt4 provides mechanistic insights into complex intracellular coordination during nitrogen deprivation

2020 ◽  
Vol 56 (6) ◽  
pp. 1642-1663 ◽  
Author(s):  
Ben Leyland ◽  
Aliza Zarka ◽  
Shoshana Didi‐Cohen ◽  
Sammy Boussiba ◽  
Inna Khozin‐Goldberg
Keyword(s):  
High Resolution ◽  
Phaeodactylum Tricornutum ◽  
Lipid Droplets ◽  
Nitrogen Deprivation ◽  
Pennate Diatom

scholarly journals Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum

2017 ◽  
Vol 372 (1728) ◽  
pp. 20160404 ◽  
Author(s):  
Valeria Villanova ◽  
Antonio Emidio Fortunato ◽  
Dipali Singh ◽  
Davide Dal Bo ◽  
Melissa Conte ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Phaeodactylum Tricornutum ◽  
Photosynthetic Activity ◽  
Biomass Accumulation ◽  
Point Of View ◽  
Physiological Data ◽  
Pennate Diatom ◽  
Metabolic Modelling ◽  
Central Carbon ◽  
Triacylglycerol Accumulation

Diatoms are prominent marine microalgae, interesting not only from an ecological point of view, but also for their possible use in biotechnology applications. They can be cultivated in phototrophic conditions, using sunlight as the sole energy source. Some diatoms, however, can also grow in a mixotrophic mode, wherein both light and external reduced carbon contribute to biomass accumulation. In this study, we investigated the consequences of mixotrophy on the growth and metabolism of the pennate diatom Phaeodactylum tricornutum , using glycerol as the source of reduced carbon. Transcriptomics, metabolomics, metabolic modelling and physiological data combine to indicate that glycerol affects the central-carbon, carbon-storage and lipid metabolism of the diatom. In particular, provision of glycerol mimics typical responses of nitrogen limitation on lipid metabolism at the level of triacylglycerol accumulation and fatty acid composition. The presence of glycerol, despite provoking features reminiscent of nutrient limitation, neither diminishes photosynthetic activity nor cell growth, revealing essential aspects of the metabolic flexibility of these microalgae and suggesting possible biotechnological applications of mixotrophy. This article is part of the themed issue ‘The peculiar carbon metabolism in diatoms'.

scholarly journals Comparative Genomics of the Pennate Diatom Phaeodactylum tricornutum

2005 ◽  
Vol 137 (2) ◽  
pp. 500-513 ◽  
Author(s):  
Anton Montsant ◽  
Kamel Jabbari ◽  
Uma Maheswari ◽  
Chris Bowler
Keyword(s):  
Comparative Genomics ◽  
Phaeodactylum Tricornutum ◽  
Pennate Diatom

scholarly journals The architecture of lipid droplets in the diatom Phaeodactylum tricornutum

2019 ◽  
Vol 38 ◽  
pp. 101415 ◽  
Author(s):  
Josselin Lupette ◽  
Antoine Jaussaud ◽  
Khawla Seddiki ◽  
Christian Morabito ◽  
Sabine Brugière ◽  
...  
Keyword(s):  
Phaeodactylum Tricornutum ◽  
Lipid Droplets

The mechanism of regulation of photosystem I cross-section in the pennate diatom Phaeodactylum tricornutum

2020 ◽  
Author(s):  
Vasco Giovagnetti ◽  
Alexander V Ruban
Keyword(s):  
Phaeodactylum Tricornutum ◽  
Wavelength Region ◽  
Culture Conditions ◽  
Protein Synthesis Inhibitor ◽  
Synthesis Inhibitor ◽  
Pennate Diatom ◽  
Intermittent Light ◽  
Long Wavelength ◽  
Wavelength Emission ◽  
Photosynthetic Complexes

Abstract Photosystems possess distinct fluorescence emissions at low (77K) temperature. PSI emits in the long-wavelength region at ~710–740 nm. In diatoms, a successful clade of marine primary producers, the contribution of PSI-associated emission (710–717 nm) has been shown to be relatively small. However, in the pennate diatom Phaeodactylum tricornutum, the source of the long-wavelength emission at ~710 nm (F710) remains controversial. Here, we addressed the origin and modulation of F710 fluorescence in this alga grown under continuous and intermittent light. The latter condition led to a strong enhancement in F710. Biochemical and spectral properties of the photosynthetic complexes isolated from thylakoid membranes were investigated for both culture conditions. F710 emission appeared to be associated with PSI regardless of light acclimation. To further assess whether PSII could also contribute to this emission, we decreased the concentration of PSII reaction centres and core antenna by growing cells with lincomycin, a chloroplast protein synthesis inhibitor. The treatment did not diminish F710 fluorescence. Our data suggest that F710 emission originates from PSI under the conditions tested and is enhanced in intermittent light-grown cells due to increased energy flow from the FCP antenna to PSI.

Autoradiographic studies of t n/t n mouse embryos

Development ◽  
1975 ◽  
Vol 33 (3) ◽  
pp. 725-730
Author(s):  
Mary Nadijcka ◽  
Nina Hillman
Keyword(s):  
High Resolution ◽  
Neutral Lipid ◽  
Lipid Droplets ◽  
Mouse Embryos ◽  
Intracellular Lipid

High-resolution autoradiographic studies were used to determine whether t12/t12 and tw32/tw32 mouse embryos synthesize the excessive lipid which distinguishes these embryos prior to their death. The studies show that the tn homozygotes synthesize neutral lipid which is stored in intracellular lipid droplets. Cholesterol and phospholipid precursors are not incorporated into these droplets.

scholarly journals RNA Interference Silencing of a Major Lipid Droplet Protein Affects Lipid Droplet Size in Chlamydomonas reinhardtii

2009 ◽  
Vol 9 (1) ◽  
pp. 97-106 ◽  
Author(s):  
Eric R. Moellering ◽  
Christoph Benning
Keyword(s):  
Rna Interference ◽  
Chlamydomonas Reinhardtii ◽  
Droplet Size ◽  
Lipid Droplet ◽  
Lipid Droplets ◽  
Biofuel Production ◽  
Nitrogen Deprivation ◽  
Green Algal ◽  
Lipid Droplet Size ◽  
Lipid Droplet Protein

ABSTRACT Eukaryotic cells store oils in the chemical form of triacylglycerols in distinct organelles, often called lipid droplets. These dynamic storage compartments have been intensely studied in the context of human health and also in plants as a source of vegetable oils for human consumption and for chemical or biofuel feedstocks. Many microalgae accumulate oils, particularly under conditions limiting to growth, and thus have gained renewed attention as a potentially sustainable feedstock for biofuel production. However, little is currently known at the cellular or molecular levels with regard to oil accumulation in microalgae, and the structural proteins and enzymes involved in the biogenesis, maintenance, and degradation of algal oil storage compartments are not well studied. Focusing on the model green alga Chlamydomonas reinhardtii, the accumulation of triacylglycerols and the formation of lipid droplets during nitrogen deprivation were investigated. Mass spectrometry identified 259 proteins in a lipid droplet-enriched fraction, among them a major protein, tentatively designated major lipid droplet protein (MLDP). This protein is specific to the green algal lineage of photosynthetic organisms. Repression of MLDP gene expression using an RNA interference approach led to increased lipid droplet size, but no change in triacylglycerol content or metabolism was observed.

scholarly journals Ultrastructure and Membrane Traffic During Cell Division in the Marine Pennate Diatom Phaeodactylum tricornutum

Protist ◽  
2015 ◽  
Vol 166 (5) ◽  
pp. 506-521 ◽  
Author(s):  
Atsuko Tanaka ◽  
Alessandra De Martino ◽  
Alberto Amato ◽  
Anton Montsant ◽  
Benjamin Mathieu ◽  
...  
Keyword(s):  
Cell Division ◽  
Phaeodactylum Tricornutum ◽  
Membrane Traffic ◽  
Pennate Diatom

scholarly journals EFFECTS OF NITROGEN DEPRIVATION ON RATES OF UPTAKE OF NITROGENOUS COMPOUNDS BY THE DIATOM, PHAEODACTYLUM TRICORNUTUM BOHLIN

1986 ◽  
Vol 102 (1) ◽  
pp. 39-44 ◽  
Author(s):  
P. J. SYRETT ◽  
K. J. FLYNN ◽  
C. J. MOLLOY ◽  
G. K. DIXON ◽  
A. M. PEPLINSKA ◽  
...  
Keyword(s):  
Phaeodactylum Tricornutum ◽  
Nitrogen Deprivation ◽  
Nitrogenous Compounds
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