A novel 13‐plex STR typing system for individual identification and parentage testing of donkeys ( Equus asinus )

2019 ◽  
Vol 52 (2) ◽  
pp. 290-297 ◽  
Author(s):  
W. Dang ◽  
S. Shang ◽  
X. Zhang ◽  
Y. Yu ◽  
D. M. Irwin ◽  
...  
Keyword(s):  
Individual Identification ◽  
Str Typing ◽  
Parentage Testing ◽  
Equus Asinus ◽  
Typing System

Quadruplex fluorescent STR typing system (HUMVWA, HUMTH01, D21S11 and HPRT) with sequence-defined allelic ladders

1998 ◽  
Vol 94 (1-2) ◽  
pp. 39-46 ◽  
Author(s):  
Feng-Xia Xiao ◽  
Anja Gilissen ◽  
Jean-Jacques Cassiman ◽  
Ronny Decorte
Keyword(s):  
Str Typing ◽  
Typing System

scholarly journals Hypervariable polymorphic VNTR loci for parentage testing and individual identification

1990 ◽  
Vol 35 (2) ◽  
pp. 179-188 ◽  
Author(s):  
Tsuyoshi Yokoi ◽  
Masayuki Nata ◽  
Toru Odaira ◽  
Kaoru Sagisaka
Keyword(s):  
Individual Identification ◽  
Parentage Testing

A Novel Fluorescent Quadruplex STR Typing System and the Allele Frequency Distributions in a Thai Population

2003 ◽  
Vol 48 (1) ◽  
pp. 2002006 ◽  
Author(s):  
Takashi Yoshimoto ◽  
Toshimichi Yamamoto ◽  
Masaki Mizutani ◽  
Rieko Uchihi ◽  
Hiroyuki Ohtaki ◽  
...  
Keyword(s):  
Allele Frequency ◽  
Str Typing ◽  
Thai Population ◽  
Frequency Distributions ◽  
Typing System

scholarly journals Evaluation of 17 microsatellite markers for parentage testing and individual identification of domestic yak (Bos grunniens)

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5946 ◽  
Author(s):  
Jie Pei ◽  
Pengjia Bao ◽  
Min Chu ◽  
Chunnian Liang ◽  
Xuezhi Ding ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Polymorphic Information Content ◽  
Domestic Animal ◽  
Parentage Analysis ◽  
The Other ◽  
Individual Identification ◽  
Parentage Testing ◽  
Bos Grunniens ◽  
Hardy Weinberg Equilibrium

Background Yak (Bos grunniens) is the most important domestic animal for people living at high altitudes. Yak ordinarily feed by grazing, and this behavior impacts the accuracy of the pedigree record because it is difficult to control mating in grazing yak. This study aimed to evaluate the pedigree system and individual identification in polled yak. Methods A total of 71 microsatellite loci were selected from the literature, mostly from the studies on cattle. A total of 35 microsatellite loci generated excellent PCR results and were evaluated for the parentage testing and individual identification of 236 unrelated polled yaks. A total of 17 of these 35 microsatellite loci had polymorphic information content (PIC) values greater than 0.5, and these loci were in Hardy–Weinberg equilibrium without linkage disequilibrium. Results Using multiplex PCR, capillary electrophoresis, and genotyping, very high exclusion probabilities were obtained for the combined core set of 17 loci. The exclusion probability (PE) for one candidate parent when the genotype of the other parent is not known was 0.99718116. PE for one candidate parent when the genotype of the other parent is known was 0.99997381. PE for a known candidate parent pair was 0.99999998. The combined PEI (PE for identity of two unrelated individuals) and PESI (PE for identity of two siblings) were >0.99999999 and 0.99999899, respectively. These findings indicated that the combination of 17 microsatellite markers could be useful for efficient and reliable parentage testing and individual identification in polled yak. Discussion Many microsatellite loci have been investigated for cattle paternity testing. Nevertheless, these loci cannot be directly applied to yak identification because the two bovid species have different genomic sequences and organization. A total of 17 loci were selected from 71 microsatellite loci based on efficient amplification, unambiguous genotyping, and high PIC values for polled yaks, and were suitable for parentage analysis in polled yak populations.

Development of a rapid 21-plex autosomal STR typing system for forensic applications

2016 ◽  
Vol 37 (21) ◽  
pp. 2789-2799 ◽  
Author(s):  
Meng Yang ◽  
Caiyong Yin ◽  
Yuexin Lv ◽  
Yaran Yang ◽  
Jing Chen ◽  
...  
Keyword(s):  
Str Typing ◽  
Autosomal Str ◽  
Typing System

scholarly journals Population genetics of 30 insertion/deletion polymorphisms in the Kuwaiti population

2019 ◽  
Vol 134 (3) ◽  
pp. 985-986
Author(s):  
Mahdi Haidar ◽  
Hussain Alsaleh ◽  
Penelope R. Haddrill
Keyword(s):  
Population Genetics ◽  
Individual Identification ◽  
Match Probability ◽  
Insertion And Deletion ◽  
Indel Markers ◽  
Typing System ◽  
Hardy Weinberg Equilibrium ◽  
Kuwaiti Population ◽  
Forensic Markers ◽  
Paternity Cases

AbstractThis study evaluates the forensic utility of the 30 insertion and deletion (indel) markers contained in the Qiagen Investigator® DIPplex kit in the Kuwaiti population (n = 150). All but one of the 30 markers were shown to conform to the expectations of the Hardy-Weinberg Equilibrium. Linkage disequilibrium tests showed no statistically significant deviation from independence. The high combined power of discrimination (CPD > 99.999%) and low combined match probability (CMP) of 2.736 × 10−13 provide a satisfactory level of discrimination, allowing the DIPplex loci to be used as forensic markers for individual identification in Kuwait. The paternity indices indicate the usefulness of the DIPplex kit as a supplementary typing system for challenging paternity cases in Kuwait.

Development and validation of a novel 29-plex Y-STR typing system for forensic application

2020 ◽  
Vol 44 ◽  
pp. 102169 ◽  
Author(s):  
Min Li ◽  
Wei Zhou ◽  
Yilun Zhang ◽  
Lei Huang ◽  
Xinjie Wang ◽  
...  
Keyword(s):  
Forensic Application ◽  
Str Typing ◽  
Typing System ◽  
Development And Validation
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