scholarly journals Hypervariable polymorphic VNTR loci for parentage testing and individual identification

1990 ◽  
Vol 35 (2) ◽  
pp. 179-188 ◽  
Author(s):  
Tsuyoshi Yokoi ◽  
Masayuki Nata ◽  
Toru Odaira ◽  
Kaoru Sagisaka
Keyword(s):  
Individual Identification ◽  
Parentage Testing

A novel 13‐plex STR typing system for individual identification and parentage testing of donkeys ( Equus asinus )

2019 ◽  
Vol 52 (2) ◽  
pp. 290-297 ◽  
Author(s):  
W. Dang ◽  
S. Shang ◽  
X. Zhang ◽  
Y. Yu ◽  
D. M. Irwin ◽  
...  
Keyword(s):  
Individual Identification ◽  
Str Typing ◽  
Parentage Testing ◽  
Equus Asinus ◽  
Typing System

scholarly journals Evaluation of 17 microsatellite markers for parentage testing and individual identification of domestic yak (Bos grunniens)

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5946 ◽  
Author(s):  
Jie Pei ◽  
Pengjia Bao ◽  
Min Chu ◽  
Chunnian Liang ◽  
Xuezhi Ding ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Polymorphic Information Content ◽  
Domestic Animal ◽  
Parentage Analysis ◽  
The Other ◽  
Individual Identification ◽  
Parentage Testing ◽  
Bos Grunniens ◽  
Hardy Weinberg Equilibrium

Background Yak (Bos grunniens) is the most important domestic animal for people living at high altitudes. Yak ordinarily feed by grazing, and this behavior impacts the accuracy of the pedigree record because it is difficult to control mating in grazing yak. This study aimed to evaluate the pedigree system and individual identification in polled yak. Methods A total of 71 microsatellite loci were selected from the literature, mostly from the studies on cattle. A total of 35 microsatellite loci generated excellent PCR results and were evaluated for the parentage testing and individual identification of 236 unrelated polled yaks. A total of 17 of these 35 microsatellite loci had polymorphic information content (PIC) values greater than 0.5, and these loci were in Hardy–Weinberg equilibrium without linkage disequilibrium. Results Using multiplex PCR, capillary electrophoresis, and genotyping, very high exclusion probabilities were obtained for the combined core set of 17 loci. The exclusion probability (PE) for one candidate parent when the genotype of the other parent is not known was 0.99718116. PE for one candidate parent when the genotype of the other parent is known was 0.99997381. PE for a known candidate parent pair was 0.99999998. The combined PEI (PE for identity of two unrelated individuals) and PESI (PE for identity of two siblings) were >0.99999999 and 0.99999899, respectively. These findings indicated that the combination of 17 microsatellite markers could be useful for efficient and reliable parentage testing and individual identification in polled yak. Discussion Many microsatellite loci have been investigated for cattle paternity testing. Nevertheless, these loci cannot be directly applied to yak identification because the two bovid species have different genomic sequences and organization. A total of 17 loci were selected from 71 microsatellite loci based on efficient amplification, unambiguous genotyping, and high PIC values for polled yaks, and were suitable for parentage analysis in polled yak populations.

scholarly journals Evaluation of a Custom SNP Panel for Identifying and Rectifying of Misjudged Paternity in Deficiency Cases

2021 ◽  
Vol 12 ◽  
Author(s):  
Liao Chang ◽  
Huiyun Yu ◽  
Xinyao Miao ◽  
Siqi Wen ◽  
Bao Zhang ◽  
...  
Keyword(s):  
Individual Identification ◽  
Close Relative ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Parentage Testing ◽  
Snp Panel ◽  
Custom Panel ◽  
Close Relatives ◽  
Short Tandem ◽  
Deficiency Cases

Parentage testing is routinely performed by genotyping short tandem repeat (STR) through capillary electrophoresis in the present. However, ambiguous or even misjudged paternity based on STRs happens from time to time in cases where only one putative parent is available. We analyzed STR data of 7,818,969 unrelated pairs and 75 close-relative pairs and found that although the probability of a random false match between non-relatives was 4.22 × 10–6, the incidence of false or ambiguous paternity results between children and first-degree relatives of their true parent was as high as 18.67%. These results highlight the risk of false inclusion of a relative or even non-relatives in parentage testing with STRs. We then validated all ambiguous STR results by targeted sequencing with a custom panel containing 4,830 individual identification single nucleotide polymorphisms (IISNP), found that the ratio of mismatch loci to total SNPs was 1.78–6.95% in close relatives compared with 10.93–13.49% in unrelated pairs. Last, we reported three real cases with undetermined paternity by STRs and rectified them by dissecting with our IISNP panel. These results suggested that high-density IISNP panel can be used to identify and rectify misjudged cases effectively.

scholarly journals Use of Microsatellites in Genetic Diversity Assessment, Parentage Testing and Individual Identification of the Kangal Shepherd Dog

2020 ◽  
Vol 70 (2) ◽  
pp. 170-181
Author(s):  
Dimitrijević Vladimir ◽  
Ristanić Marko ◽  
Stanisić Ljubodrag ◽  
Drobnjak Darko ◽  
Urosević Milivoje ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Polymorphic Information Content ◽  
Mean Value ◽  
Individual Identification ◽  
Parentage Testing ◽  
Diversity Assessment ◽  
Study Population ◽  
The Mean ◽  
Matching Probability

AbstractThe Kangal Shepherd Dog is considered the most common dog breed of Turkish origin. This study investigated variations in ten autosomal microsatellite markers (PEZ01, PEZ03, PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 and FHC2079) for the purposes of genetic diversity assessment of the Kangal breed. In addition, the use of markers was assessed in parentage testing and individual identification within the Kangal breed. The microsatellite markers were typed in 51 Kangal dogs. The total number of alleles in the study population was 69. The mean number of alleles per locus was 6.9, and varied from four (FHC2079) to 12 (PEZ12). The polymorphic information content (PIC) ranged from 0.52 (FHC2079) to 0.87 (PEZ12), with the mean value for all loci of 0.717. Power of exclusion (PE) in 10 microsatellites investigated varied between 0.143 (FHC2054) and 0.472 (PEZ08) per locus. In order to determine the efficiency of using microsatellites for individual identification in the Kangal breed, power of discrimination (PD) and matching probability (MP) were calculated for each microsatellite marker. The panel achieved high combined MP (6.77 × 10-10) and high combined PD value of 99.99999%. The obtained results may contribute to further recognition of the Kangal breed, and confirm that the investigated microsatellites enable a reliable parentage testing and individual identification of the breed.

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