Development of a rapid 21-plex autosomal STR typing system for forensic applications

2016 ◽  
Vol 37 (21) ◽  
pp. 2789-2799 ◽  
Author(s):  
Meng Yang ◽  
Caiyong Yin ◽  
Yuexin Lv ◽  
Yaran Yang ◽  
Jing Chen ◽  
...  
Keyword(s):  
Str Typing ◽  
Autosomal Str ◽  
Typing System

scholarly journals Assessment of QIAGEN™ Investigator® 24plex GO! kit workflow for autosomal STR profiling of forensic reference samples

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hashom Mohd Hakim ◽  
Hussein Omar Khan ◽  
Siti Afifah Ismail ◽  
Nurul Hazirah Mat Lazim ◽  
Japareng Lalung ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Dna Profiling ◽  
Buccal Swab ◽  
Forensic Dna ◽  
Str Typing ◽  
Str Analysis ◽  
Dna Database ◽  
Autosomal Str ◽  
Dna Template ◽  
Reference Samples

Abstract Background DNA profiling has proven to be a valuable technique for identification of individuals in crime. Currently, the technique targets several short tandem repeat (STR) regions in human genome. However, increasing number of samples submitted for STR analysis may lead to delays due to the limited number of experienced analysts who might be available at any given moment and the time taken to complete lengthy DNA profiling procedures. This study was conducted to test the specificity, repeatability, reproducibility and robustness of Investigator® 24plex GO! kit for genotyping of reference samples submitted to the Royal Malaysian Police Forensic DNA Laboratory for DNA database. Material and methods In this study, Investigator® 24plex GO! kit was used to directly amplify STR loci from buccal swab cell of reference samples that had previously been STR typed using GlobalFiler™ Express kit. Capillary electrophoresis was carried out on a 3500xL Genetic Analyser using POP-4® Polymer. Amplified products were assigned to particular STR alleles using the GeneMapper ID-X version 1.4 software. Results Our study shows that STR profiles generated using Investigator® 24plex GO! gave concordance results with those previously obtained using the GlobalFiler™ Express kit. In addition, quality sensors included in the kit are of particular importance for determining the effectiveness of the PCR reaction and help to indicate the nature and quantity of DNA template for PCR amplification. Conclusion The Investigator® 24plex GO! kit is reliable for STR typing of reference samples.

A novel 13‐plex STR typing system for individual identification and parentage testing of donkeys ( Equus asinus )

2019 ◽  
Vol 52 (2) ◽  
pp. 290-297 ◽  
Author(s):  
W. Dang ◽  
S. Shang ◽  
X. Zhang ◽  
Y. Yu ◽  
D. M. Irwin ◽  
...  
Keyword(s):  
Individual Identification ◽  
Str Typing ◽  
Parentage Testing ◽  
Equus Asinus ◽  
Typing System

Fundamentals of Autosomal STR Typing for Forensic Applications: Case Studies

2018 ◽  
pp. 209-221
Author(s):  
Hirak R. Dash ◽  
Neha Rawat ◽  
Sonia Kakkar ◽  
Arun Kumar Swain
Keyword(s):  
Case Studies ◽  
Str Typing ◽  
Autosomal Str

Prediction of autosomal STR typing success in ancient and Second World War bone samples

2017 ◽  
Vol 27 ◽  
pp. 17-26 ◽  
Author(s):  
Irena Zupanič Pajnič ◽  
Tomaž Zupanc ◽  
Jože Balažic ◽  
Živa Miriam Geršak ◽  
Oliver Stojković ◽  
...  
Keyword(s):  
Second World War ◽  
World War ◽  
Str Typing ◽  
Autosomal Str ◽  
Second World

Quadruplex fluorescent STR typing system (HUMVWA, HUMTH01, D21S11 and HPRT) with sequence-defined allelic ladders

1998 ◽  
Vol 94 (1-2) ◽  
pp. 39-46 ◽  
Author(s):  
Feng-Xia Xiao ◽  
Anja Gilissen ◽  
Jean-Jacques Cassiman ◽  
Ronny Decorte
Keyword(s):  
Str Typing ◽  
Typing System

Autosomal STR Typing and Case Studies

2020 ◽  
pp. 257-275
Author(s):  
Pankaj Shrivastava ◽  
Shivani Dixit ◽  
Varsha Srivastava ◽  
Hirak Ranjan Dash ◽  
R. K. Kumawat
Keyword(s):  
Case Studies ◽  
Str Typing ◽  
Autosomal Str

A Novel Fluorescent Quadruplex STR Typing System and the Allele Frequency Distributions in a Thai Population

2003 ◽  
Vol 48 (1) ◽  
pp. 2002006 ◽  
Author(s):  
Takashi Yoshimoto ◽  
Toshimichi Yamamoto ◽  
Masaki Mizutani ◽  
Rieko Uchihi ◽  
Hiroyuki Ohtaki ◽  
...  
Keyword(s):  
Allele Frequency ◽  
Str Typing ◽  
Thai Population ◽  
Frequency Distributions ◽  
Typing System

scholarly journals Improved autosomal STR typing of degraded femur samples extracted using a custom demineralization buffer and DNA IQ™

2021 ◽  
Vol 3 ◽  
pp. 100131
Author(s):  
Gayvelline C. Calacal ◽  
Bea G. Gallardo ◽  
Dame Loveliness T. Apaga ◽  
Maria Corazon A. De Ungria
Keyword(s):  
Str Typing ◽  
Autosomal Str

Development and validation of a novel 29-plex Y-STR typing system for forensic application

2020 ◽  
Vol 44 ◽  
pp. 102169 ◽  
Author(s):  
Min Li ◽  
Wei Zhou ◽  
Yilun Zhang ◽  
Lei Huang ◽  
Xinjie Wang ◽  
...  
Keyword(s):  
Forensic Application ◽  
Str Typing ◽  
Typing System ◽  
Development And Validation

A mini-STR typing system for degraded equine DNA

2018 ◽  
Vol 49 (5) ◽  
pp. 464-466 ◽  
Author(s):  
T. J. Kun ◽  
E. J. Wictum ◽  
M. C. T. Penedo
Keyword(s):  
Str Typing ◽  
Typing System
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