scholarly journals Tear and aqueous humour cytokine profile in primary open‐angle glaucoma

2020 ◽  
Vol 98 (6) ◽  
Author(s):  
Barbara Burgos‐Blasco ◽  
Beatriz Vidal‐Villegas ◽  
Federico Saenz‐Frances ◽  
Laura Morales‐Fernandez ◽  
Lucia Perucho‐Gonzalez ◽  
...  
Keyword(s):  
Aqueous Humour ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Cytokine Profile ◽  
Open Angle

scholarly journals Cellular and plasma fibronectin in the aqueous humour of primary open-angle glaucoma, exfoliative glaucoma and cataract patients

Eye ◽  
1998 ◽  
Vol 12 (5) ◽  
pp. 886-890 ◽  
Author(s):  
Minna Vesaluoma ◽  
Päivi Mertaniemi ◽  
Sari Mannonen ◽  
Ilkka Lehto ◽  
Risto Uusitalo ◽  
...  
Keyword(s):  
Aqueous Humour ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Open Angle ◽  
Plasma Fibronectin ◽  
Exfoliative Glaucoma ◽  
Cataract Patients

Cytokine profile in tear and aqueous humor of primary open-angle patients as a prognostic factor for trabeculectomy outcome

2021 ◽  
pp. 112067212110559
Author(s):  
Barbara Burgos-Blasco ◽  
Beatriz Vidal-Villegas ◽  
Federico Saenz-Frances ◽  
Jose I Fernandez-Vigo ◽  
Vanessa Andres-Guerrero ◽  
...  
Keyword(s):  
Aqueous Humor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Cytokine Profile ◽  
Open Angle ◽  
Fiber Layer ◽  
Nerve Fiber Layer Thickness ◽  
Cytokine Levels ◽  
Surgical Failure ◽  
One Year

Objective To evaluate the cytokine profile in tear and aqueous humor in primary open-angle glaucoma before trabeculectomy and correlate preoperative cytokine levels with the surgical outcome. Methods Prospective study. Twenty-nine patients with primary open-angle glaucoma undergoing primary trabeculectomy were included. Levels of 27 cytokines were measured in tear an aqueous humor using the Bio-Plex Pro Human Cytokine 27-Plex Immunoassay kit (Bio-Rad Laboratories, Hercules, CA, USA). Results 29 patients who underwent trabeculectomy were included and their first-year follow-up visits were recorded. Mean age was 76.0 ± 7.0 years (range 56–84), mean intraocular pressure was 18.2 ± 3.6 mmHg and mean number of topical medications was 2.3 ± 0.9. At the one-year visit, 5 patients were classified as surgical failure. In aqueous humor, preoperative cytokine levels of regulated on activation normal T cell expressed and secreted (RANTES) were significantly higher in those patients with surgical failure at one year. IL-8 in tear and interferon gamma-induced protein (IP-10) in aqueous humor correlated positively with one-year IOP reduction. No statistically significant correlations were found with changes in visual field mean defect or global peripapillary retinal nerve fiber layer thickness (all, p >0.05). Conclusions Preoperative RANTES levels in aqueous humor as well as other cytokines could serve as useful biomarkers for trabeculectomy outcome.

scholarly journals SELENIUM AND SELENOPROTEIN P1 LEVELS ARE RELATED TO PRIMARY OPEN-ANGLE GLAUCOMA / NIVOI SELENA I SELENOPROTEINA P1 POVEZANI SU SA PRIMARNIM GLAUKOMOM OTVORENOG UGLA

2013 ◽  
Vol 33 (2) ◽  
pp. 143-148 ◽  
Author(s):  
Mohammad Najafi ◽  
Morteza Nouruzi Yeganeh ◽  
Arezoo Miraftabi ◽  
Reza Zarei ◽  
Isa Noormohammadi
Keyword(s):  
Aqueous Humour ◽  
Primary Open Angle Glaucoma ◽  
Reduced Glutathione ◽  
Open Angle Glaucoma ◽  
Serum Levels ◽  
Visual Field Defects ◽  
Unknown Etiology ◽  
Serum Selenium ◽  
Open Angle ◽  
Significant Difference

Summary Background Glaucoma is a highly prevalent eye disease related to optic nerve lesions and visual field defects. Primary Open-Angle Glaucoma (POAG) is a type of glaucoma that occurs frequently with unknown etiology. In this study, we investigated the serum levels of selenium, selenoprotein P1, glutathione, hemolysate glutathione peroxidase1 (GPx1) activity and aqueous humour selenium in POAG patients. Methods Ninety sex- and age-matched subjects (POAG patients; n=45 and, controls; n=45) with the controlled confounders (smoking, hypertension and alcohol beverages) were recruited on clinical histories and exams. The serum and aqueous humour selenium levels were measured using GFAAS technique. The serum selenoprotein P1 level was assayed with the ELISA method. The hemolysate GPx1 activity and serum reduced glutathione level were also measured using known colorimetric techniques. Results The serum selenium (P=0.01) and selenoprotein P1 (P<0.001) levels were significantly high in POAG patients. Furthermore, the aqueous humour selenium level was significantly high among patients as compared to controls (64.68±13.07 vs. 58.36±13.76 ng/mL, P=0.02). The results did not show a significant difference (P=0.36) in the hemolysate GPx1 activity between the groups. The cutoff points for intraocular pressure (IOP) and serum selenoprotein P1 parameters were estimated to be 39 mmHg (sensitivity 97.5%; 1-specificity 6.5%) and 188 mg/mL (sensitivity 93.5%; 1-specificity 14%), respectively.

scholarly journals A study of aqueous humour proteins in patients of primary open angle glaucoma

2010 ◽  
Vol 01 (02) ◽  
pp. 110-114 ◽  
Author(s):  
Meena Zaidi ◽  
Azra Jilani ◽  
Padmawati Bhattacharya ◽  
Najmul Islam ◽  
Shahid Alam
Keyword(s):  
Aqueous Humour ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Open Angle

scholarly journals Low expression of GSTP1 in the aqueous humour of patients with primary open‐angle glaucoma

2021 ◽  
Vol 25 (6) ◽  
pp. 3063-3079
Author(s):  
Aihua Liu ◽  
Liming Wang ◽  
Qiang Feng ◽  
Dandan Zhang ◽  
Kexi Chen ◽  
...  
Keyword(s):  
Aqueous Humour ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Open Angle ◽  
Low Expression

scholarly journals Cytokine profile in tear and aqueous humor of primary open‐angle glaucoma patients as a prognostic factor for trabeculectomy outcome

2022 ◽  
Vol 100 (S267) ◽  
Author(s):  
Barbara Burgos‐Blasco ◽  
Beatriz Vidal ◽  
Federico Sáenz‐Francés ◽  
Jose Ignacio Fernandez‐Vigo ◽  
Laura Espino ◽  
...  
Keyword(s):  
Prognostic Factor ◽  
Aqueous Humor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Cytokine Profile ◽  
Open Angle

Freeze-controlled Crystallisation of the Aqueous Humour in Glaucomatous and Non-glaucomatous Patients

2010 ◽  
Vol 04 (01) ◽  
pp. 29
Author(s):  
Christoph Faschinger ◽  
Franz Rabensteiner Dieter ◽  
Christian Eberle ◽  
Georg Mossböck ◽  
Otto Schmut ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Trabecular Meshwork ◽  
Aqueous Humour ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Pigmentary Glaucoma ◽  
Multifactorial Disease ◽  
Open Angle ◽  
Microscope Slide ◽  
Trabecular Meshwork Cells

The purpose of this study was to investigate the aqueous humour of glaucomatous and non-glaucomatous patients by freeze-controlled crystallisation qualitatively to detect a possible difference. Aqueous humour was taken from 20 patients with different kinds of glaucoma (11 primary open-angle glaucoma, eight pseudo-exfoliation glaucoma, one pigmentary glaucoma) and compared with samples from 40 patients with cataracts and to samples from seven patients with cataract and glaucoma; 1μl of each sample was applied to a microscope slide and put in a deep-freezer at a constant temperature of -20°C for 24 hours. During this time, the water evaporated and distinct crystal patterns developed. These patterns were examined stereo-microscopically, stored digitally and compared with each other. Many distinct crystals of different sizes and formations were obtained. No obvious differences in the crystal patterns could be found between the three groups (patients with glaucoma, cataract or cataract and glaucoma). As a multifactorial disease, open-angle glaucoma is characterised by specific changes to the trabecular meshwork cells with an accumulation of deposits of extracellular matrix. Since the trabeculum is rinsed consistently by aqueous humour, changes of the compounds might influence these cells. Alterations concerning this were published. In contrast to the ferning test, one of several methods of examining fluids with patterns that are highly dependent on temperature and humidity, our examinations with freeze-controlled crystallisation at a constant and controlled temperature of – 20°C gave more valid results. In our study we could not find obvious differences in the crystal patterns of patients with cataract, glaucoma or cataract and glaucoma.

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