Microsphere suspension array technology for SNP detection in cattle

2003 ◽  
Vol 22 (4) ◽  
pp. 158-162 ◽  
Author(s):  
S. Dunbar ◽  
R. Godbout ◽  
H. Newkirk ◽  
J. Hetzel
Keyword(s):  
Snp Detection ◽  
Suspension Array ◽  
Array Technology

scholarly journals Magnetic Suspension Array Technology: Controlled Synthesis and Screening in Microfluidic Networks

Small ◽  
2016 ◽  
Vol 12 (33) ◽  
pp. 4553-4562 ◽  
Author(s):  
Gungun Lin ◽  
Dmitriy D. Karnaushenko ◽  
Gilbert Santiago Cañón Bermúdez ◽  
Oliver G. Schmidt ◽  
Denys Makarov
Keyword(s):  
Magnetic Suspension ◽  
Controlled Synthesis ◽  
Suspension Array ◽  
Array Technology ◽  
Microfluidic Networks

Simultaneous detection of five antibiotics in milk by high-throughput suspension array technology

Talanta ◽  
2011 ◽  
Vol 85 (2) ◽  
pp. 1160-1165 ◽  
Author(s):  
Pu Su ◽  
Nan Liu ◽  
Maoxiang Zhu ◽  
Baoan Ning ◽  
Ming Liu ◽  
...  
Keyword(s):  
High Throughput ◽  
Simultaneous Detection ◽  
Suspension Array ◽  
Array Technology

scholarly journals Prevalence and clinical impact of malaria infections detected with a highly sensitive HRP2 rapid diagnostic test in Beninese pregnant women

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Valérie Briand ◽  
Gilles Cottrell ◽  
Nicaise Tuike Ndam ◽  
Xavier Martiáñez-Vendrell ◽  
Bertin Vianou ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Parasite Density ◽  
Quantitative Polymerase Chain Reaction ◽  
Suspension Array ◽  
Increased Risk ◽  
Array Technology ◽  
Highly Sensitive ◽  
Polymerase Chain

Abstract Background While sub-microscopic malarial infections are frequent and potentially deleterious during pregnancy, routine molecular detection is still not feasible. This study aimed to assess the performance of a Histidine Rich Protein 2 (HRP2)-based ultrasensitive rapid diagnostic test (uRDT, Alere Malaria Ag Pf) for the detection of infections of low parasite density in pregnant women. Methods This was a retrospective study based on samples collected in Benin from 2014 to 2017. A total of 942 whole blood samples collected in 327 women in the 1st and 3rd trimesters and at delivery were tested by uRDT, conventional RDT (cRDT, SD BIOLINE Malaria Ag Pf), microscopy, quantitative polymerase chain-reaction (qPCR) and Luminex-based suspension array technology targeting P. falciparum HRP2. The performance of each RDT was evaluated using qPCR as reference standard. The association between infections detected by uRDT, but not by cRDT, with poor maternal and birth outcomes was assessed using multivariate regression models. Results The overall positivity rate detected by cRDT, uRDT, and qPCR was 11.6% (109/942), 16.2% (153/942) and 18.3% (172/942), respectively. Out of 172 qPCR-positive samples, 68 were uRDT-negative. uRDT had a significantly better sensitivity (60.5% [52.7–67.8]) than cRDT (44.2% [36.6–51.9]) and a marginally decreased specificity (93.6% [91.7–95.3] versus 95.7% [94.0–97.0]). The gain in sensitivity was particularly high (33%) and statistically significant in the 1st trimester. Only 28 (41%) out of the 68 samples which were qPCR-positive, but uRDT-negative had detectable but very low levels of HRP2 (191 ng/mL). Infections that were detected by uRDT but not by cRDT were associated with a 3.4-times (95%CI 1.29–9.19) increased risk of anaemia during pregnancy. Conclusions This study demonstrates the higher performance of uRDT, as compared to cRDTs, to detect low parasite density P. falciparum infections during pregnancy, particularly in the 1st trimester. uRDT allowed the detection of infections associated with maternal anaemia.

Abstract 3456: Assessment of Genetic Risk for Hypertriglyceridemia

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Kimihiko Kato ◽  
Mitsutoshi Oguri ◽  
Tetsuro Yoshida ◽  
Takeshi Hibino ◽  
Kazuhiro Yajima ◽  
...  
Keyword(s):  
Genetic Risk ◽  
Serum Triglyceride ◽  
Community Dwelling ◽  
Multivariable Logistic Regression Analysis ◽  
Chi Square ◽  
Suspension Array ◽  
Array Technology ◽  
Chi Square Test ◽  
Polymerase Chain ◽  
Subject Panel

Introduction. Hypertriglyceridemia is an important risk factor for coronary heart disease. The purpose of the present study was to identify gene polymorphisms associated with hypertriglyceridemia (serum triglyceride concentration, ≥1.65 mmol/L) for assessment of the genetic risk for this condition. Methods. A total of 5206 individuals from two independent populations was examined: Subject panel A comprised 3787 individuals who either visited outpatient clinics of or were admitted to the participating hospitals because of various symptoms or for a health checkup; subject panel B comprised 1419 community-dwelling elderly individuals. The genotypes for 100 polymorphisms of 65 candidate genes were determined by a method that combines the polymerase chain reaction and sequence-specific oligonucleotide probes with suspension array technology. Given the multiple comparisons of genotypes with hypertriglyceridemia, we adopted the criterion of a false discovery rate (FDR) of <0.05 for significant association in initial screening with the chi-square test. Results. Evaluation of genotype distributions by the chi-square test and subsequent multivariable logistic regression analysis with adjustment for age and sex revealed that seven polymorphisms [−1131T→ C, −3A→G, and 553G→T (Gly185Cys) of APOA5 ; 1100C→T of APOC3 ; 85T→C of APOA1 ; 41A→G (Glu14Gly) of ACAT2 ; C→G (Ser47Stop) of LPL ] were significantly (FDR < 0.05) associated with hypertriglyceridemia in subject panel A. To validate these associations, we examined the same polymorphisms in subject panel B. The six polymorphisms of APOA5 , APOC3 , APOA1 , and LPL , but not that of ACAT2 , were again significantly associated with hypertriglyceridemia. Serum triglyceride concentrations differed significantly ( P< 0.05, ANOVA) among genotypes of each of these six polymorphisms in both subject panels. The three polymorphisms of APOA5 were in linkage disequilibrium. Conclusions. Polymorphisms of APOA5 , APOC3 , APOA1 , and LPL are determinants of hypertriglyceridemia. Genotyping of these polymorphisms may prove informative for assessment of the genetic risk for hypertriglyceridemia and may contribute to the personalized prevention of this condition.

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