scholarly journals Rapid, Multiplexed Characterization of Shiga Toxin-Producing Escherichia coli (STEC) Isolates Using Suspension Array Technology

2016 ◽  
Vol 7 ◽  
Author(s):  
John M. Carter ◽  
Andrew Lin ◽  
Laurie Clotilde ◽  
Matthew Lesho
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Suspension Array ◽  
Array Technology

scholarly journals Whole-genome characterization of hemolytic uremic syndrome-causing Shiga toxin-producing Escherichia coli in Sweden

Virulence ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 1296-1305
Author(s):  
Ying Hua ◽  
Milan Chromek ◽  
Anne Frykman ◽  
Cecilia Jernberg ◽  
Valya Georgieva ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hemolytic Uremic Syndrome ◽  
Shiga Toxin ◽  
Whole Genome ◽  
Genome Characterization ◽  
Uremic Syndrome

scholarly journals Comparative Genomics and Characterization of the Late Promoter pR’ from Shiga Toxin Prophages in Escherichia coli

Viruses ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 595 ◽  
Author(s):  
Ling Zhang ◽  
David Simpson ◽  
Lynn McMullen ◽  
Michael Gänzle
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Toxin Production ◽  
Late Promoter ◽  
Regulatory Systems ◽  
Human Illness ◽  
Late Transcription ◽  
Native Strains ◽  
Native Host

Shiga-toxin producing Escherichia coli (STEC) causes human illness ranging from mild diarrhea to death. The bacteriophage encoded stx genes are located in the late transcription region, downstream of the antiterminator Q. The transcription of the stx genes is directly under the control of the late promoter pR’, thus the sequence diversity of the region between Q and stx, here termed the pR’ region, may affect Stx toxin production. Here, we compared the gene structure of the pR’ region and the stx subtypes of nineteen STECs. The sequence alignment and phylogenetic analysis suggested that the pR’ region tends to be more heterogeneous than the promoter itself, even if the prophages harbor the same stx subtype. Furthermore, we established and validated transcriptional fusions of the pR’ region to the DsRed reporter gene using mitomycin C (MMC) induction. Finally, these constructs were transformed into native and non-native strains and examined with flow cytometry. The results showed that induction levels changed when pR’ regions were placed under different regulatory systems. Moreover, not every stx gene could be induced in its native host bacteria. In addition to the functional genes, the diversity of the pR’ region plays an important role in determining the level of toxin induction.

scholarly journals Characterization of Fecal Shiga Toxin-Producing Escherichia coli from Post-Weaning Cattle

2019 ◽  
Vol 3 (2) ◽  
pp. 147-147
Author(s):  
F. Yang ◽  
S. Klopotek ◽  
J. Oltjen ◽  
X. Yang
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin

scholarly journals Characterization of Shiga toxin-producing Escherichia coli isolated from cattle and their contacts

2021 ◽  
Vol 22 (1) ◽  
pp. 13-19
Author(s):  
Mayada Gwida ◽  
shimaa EL Mahmoudy ◽  
Adel Elgohary ◽  
Amro Mohamed ◽  
Hazem Hassan
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin

Comparative virulence characterization of the Shiga toxin phage-cured Escherichia coli O104:H4 and enteroaggregative Escherichia coli

2018 ◽  
Vol 308 (7) ◽  
pp. 912-920 ◽  
Author(s):  
Nadja Haarmann ◽  
Michael Berger ◽  
Ivan U. Kouzel ◽  
Alexander Mellmann ◽  
Petya Berger
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin
Sign in / Sign up

Export Citation Format

Share Document