scholarly journals Binding of Spermidine to a Unique Protein in Thin-Layer Tobacco Tissue Culture

1988 ◽  
Vol 88 (4) ◽  
pp. 996-998 ◽  
Author(s):  
Akiva Apelbaum ◽  
Zoe N. Canellakis ◽  
Philip B. Applewhite ◽  
Ravindar Kaur-Sawhney ◽  
Arthur W. Galston
Keyword(s):  
Tissue Culture ◽  
Thin Layer ◽  
Unique Protein ◽  
Tobacco Tissue Culture

The relationship of morphogenetic potency of tobacco tissue culture and its cytogenetic features

1974 ◽  
Vol 16 (4) ◽  
pp. 262-274 ◽  
Author(s):  
Nediyalka A. Zagorska ◽  
Zlata B. Shamina ◽  
Raisa G. Butenko
Keyword(s):  
Tissue Culture ◽  
Tobacco Tissue Culture ◽  
Relationship Of ◽  
The Relationship

scholarly journals Culture conditions for nicotine production in tobacco tissue culture.

1978 ◽  
Vol 42 (6) ◽  
pp. 1245-1251 ◽  
Author(s):  
Shinsuke OHTA ◽  
Osamu MATSUI ◽  
Michihiko YATAZAWA
Keyword(s):  
Tissue Culture ◽  
Culture Conditions ◽  
Tobacco Tissue Culture

Culture Conditions for Nicotine Production in Tobacco Tissue Culture

1978 ◽  
Vol 42 (6) ◽  
pp. 1245-1251
Author(s):  
Shinsuke Ohta ◽  
Osamu Matsui ◽  
Michihiko Yatazawa
Keyword(s):  
Tissue Culture ◽  
Culture Conditions ◽  
Tobacco Tissue Culture

Chromatin changes related to dedifferentiation and differentiation in tobacco tissue culture (Nicotiana tabacum L.)

Planta ◽  
1982 ◽  
Vol 155 (4) ◽  
pp. 273-280 ◽  
Author(s):  
Jos� Guerri ◽  
Francisco Culia�ez ◽  
Eduardo Primo-Millo ◽  
Eduardo Primo-Y�fera
Keyword(s):  
Tissue Culture ◽  
Nicotiana Tabacum ◽  
Nicotiana Tabacum L ◽  
Tobacco Tissue Culture

Antifungal compounds in aspen: effect of water stress

1994 ◽  
Vol 72 (4) ◽  
pp. 454-460 ◽  
Author(s):  
Brian M. Kruger ◽  
Paul D. Manion
Keyword(s):  
Tissue Culture ◽  
Water Stress ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Clonal Variation ◽  
Antifungal Compounds ◽  
Inhibitory Compounds ◽  
Hypoxylon Mammatum ◽  
Layer Chromatography ◽  
Tissue Culture Plantlets

A thin layer chromatography bioassay was used to detect antifungal compounds in tissue culture plantlets and potted seedlings of aspen. Catechol and the phenolic glycosides salicin and salicortin were identified as inhibitory compounds; a fourth compound was observed but was not identified. Inhibitory compound levels were estimated in eight tissue culture clones grown on unamended media and media amended with 0.22 M mannitol to induce water stress. Aspen tissue culture plantlets grown under water stress conditions had significantly lower levels of catechol, salicortin, and salicin. Significant clonal variation in levels of catechol, salicin, and the unidentified compound was also observed. Catechol, salicortin, and salicin were inhibitory to Hypoxylon mammatum when tested at levels similar to those employed in the thin layer chromatography bioassay. These results suggest that a reduction in the levels of inhibitory compounds in water-stressed aspen may be a factor in the water stress induced susceptibility of aspen to H. mammatum. Key words: Populus tremuloides, Hypoxylon mammatum, water stress, tissue culture.

Starch-enhanced synthesis and release of amylolytic enzymes from normal and crown gall tumor tobacco tissue culture cells

1982 ◽  
Vol 60 (8) ◽  
pp. 1474-1478 ◽  
Author(s):  
Charles Voliva ◽  
Gustave W. Moessen ◽  
Ann G. Matthysse
Keyword(s):  
Tissue Culture ◽  
Carbon Source ◽  
Tumor Cells ◽  
Crown Gall ◽  
Amylolytic Activity ◽  
Amylolytic Enzymes ◽  
Crown Gall Tumor ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Tobacco Tissue Culture

The response of tobacco crown gall tumor cells growing in tissue culture to a substitution of starch for sucrose as the carbon source in the medium was compared with the response of normal tobacco tissue culture cells. In both cases amylolytic activity was secreted into the medium. The increase in extracellular amylolytic activity was preceded by an increase in intracellular activity. The increase in intracellular amylolytic enzymes was sensitive to cycloheximide and to actinomycin D indicating that enzyme induction at the level of mRNA synthesis was required. No significant difference between the responses of normal and tumor cells was observed. Thus crown gall tumor cells were as capable as normal cells of sensing and responding to an alteration in the carbon source in the external medium.

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