Starch-enhanced synthesis and release of amylolytic enzymes from normal and crown gall tumor tobacco tissue culture cells

1982 ◽  
Vol 60 (8) ◽  
pp. 1474-1478 ◽  
Author(s):  
Charles Voliva ◽  
Gustave W. Moessen ◽  
Ann G. Matthysse
Keyword(s):  
Tissue Culture ◽  
Carbon Source ◽  
Tumor Cells ◽  
Crown Gall ◽  
Amylolytic Activity ◽  
Amylolytic Enzymes ◽  
Crown Gall Tumor ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Tobacco Tissue Culture

The response of tobacco crown gall tumor cells growing in tissue culture to a substitution of starch for sucrose as the carbon source in the medium was compared with the response of normal tobacco tissue culture cells. In both cases amylolytic activity was secreted into the medium. The increase in extracellular amylolytic activity was preceded by an increase in intracellular activity. The increase in intracellular amylolytic enzymes was sensitive to cycloheximide and to actinomycin D indicating that enzyme induction at the level of mRNA synthesis was required. No significant difference between the responses of normal and tumor cells was observed. Thus crown gall tumor cells were as capable as normal cells of sensing and responding to an alteration in the carbon source in the external medium.

In vitro measurement of cytotoxic antibodies in mouse-immune sera against mouse ascites tumor cells

1974 ◽  
Vol 20 (12) ◽  
pp. 1681-1688 ◽  
Author(s):  
C. P. Eng ◽  
W. R. Tolbert ◽  
J. B. Harnaha ◽  
J. P. Concannon
Keyword(s):  
Tissue Culture ◽  
Tumor Cells ◽  
Specific Antigen ◽  
Rabbit Serum ◽  
In Vitro Test ◽  
Ascites Tumor ◽  
Serum Samples ◽  
Culture Cells ◽  
Tissue Culture Cells

Previous studies reported that nontumorigenic 6C3HED tissue-culture cells induced strong tumor-specific immunoprotection against 6C3HED tumors. However, in the in vitro test, sera from C3H mice immunized with nontumorigenic 6C3HED tissue-culture cells failed to lyse 6C3HED ascites tumor cells when fresh guinea pig serum was incorporated as the complement source. A number of serum samples from different animal species were assayed as a complement source. With the exception of fresh rabbit serum, all other sera were either by themselves toxic to 6C3HED ascites tumor cells, or did not function as a complement source. The rabbit serum, by itself, was not toxic to 6C3HED tumor cells, but when incorporated with the isogeneic mouse-immune serum, killed more than 95% tumor cells. The facilitating activity exhibited by rabbit serum was characterized as classical complement and was not due to the presence of heterophile antibodies in the rabbit serum. By using this immunolytic testing system, the sera from mice bearing 6C3HED, S-180, TA-3, and Ehrlich solid tumors for an extensive time were found toxic to homologous ascites tumor cells. The cross-reactivity of the sera from tumor-bearing animals suggests that these four murine tumors, 6C3HED, S-180, TA-3, and Ehrlich, share some common tumor-specific antigen(s).

Uptake of potassium ions by normal and crown-gall-tumor cells of Vinca rosea grown in tissue culture

Planta ◽  
1979 ◽  
Vol 146 (2) ◽  
pp. 113-117 ◽  
Author(s):  
Constance Bouricius Lentz ◽  
Thomas K. Hodges ◽  
Ann G. Matthysse
Keyword(s):  
Tissue Culture ◽  
Tumor Cells ◽  
Crown Gall ◽  
Potassium Ions ◽  
Crown Gall Tumor ◽  
Vinca Rosea

Cytoplasmic Tubules in Cells Infected with Laryngotracheitis Virus

1969 ◽  
Vol 27 ◽  
pp. 376-377
Author(s):  
A. M. Watrach
Keyword(s):  
Tissue Culture ◽  
Small Proportion ◽  
Chicken Embryo ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Morphologic Characteristics ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
In Cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

Sulfhydryl bond formation is a prerequisite for proper cycling of centrosomes and chromosomes in mammalian tissue culture cells

1993 ◽  
Vol 51 ◽  
pp. 342-343
Author(s):  
Heide Schatten ◽  
Neidhard Paweletz ◽  
Ron Balczon
Keyword(s):  
Tissue Culture ◽  
Cell Cycle Progression ◽  
Group Formation ◽  
Sulfhydryl Group ◽  
Mammalian Tissue ◽  
Mitotic Chromosomes ◽  
Microtubule Network ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Transmission Electron

To study the role of sulfhydryl group formation during cell cycle progression, mammalian tissue culture cells (PTK2) were exposed to 100¼M 2-mercaptoethanol for 2 to 6 h during their exponential phase of growth. The effects of 2-mercaptoethanol on centrosomes, chromosomes, microtubules, membranes and intermediate filaments were analyzed by transmission electron microscopy (TEM) and by immunofluorescence microscopy (IFM) methods using a human autoimmune antibody directed against centrosomes (SPJ), and a mouse monoclonal antibody directed against tubulin (E7). Chromosomes were affected most by this treatment: premature chromosome condensation was detected in interphase nuclei, and the structure in mitotic chromosomes was altered compared to control cells. This would support previous findings in dividing sea urchin cells in which chromosomes are arrested at metaphase while the centrosome splitting cycle continues. It might also support findings that certairt-sulfhydryl-blocking agents block cyclin destruction. The organization of the microtubule network was scattered probably due to a looser organization of centrosomal material at the interphase centers and at the mitotic poles.

UDP-D-glucose 4-epimerase activity of the tissue culture of white poplars (Populus alba L. var. pyramidalis)

1982 ◽  
Vol 47 (5) ◽  
pp. 1530-1536 ◽  
Author(s):  
Ladislav Bilisics ◽  
Štefan Karácsonyi ◽  
Marta Kubačková
Keyword(s):  
Tissue Culture ◽  
Cell Walls ◽  
Enzyme Preparation ◽  
Uridine Diphosphate ◽  
Populus Alba ◽  
Activity Change ◽  
White Poplar ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Galactose Content

The presence of UDP-D-glucose 4-epimerase (EC 5.1.3.2) in the culture tissue of white poplar was evidenced. As found, the partially purified enzyme preparation contained UDP-D-glucose glucosyltransferase, UDP-D-galactose galactosyltransferase and non-specific enzymes able to cleave the uridine-diphosphate saccharides into the appropriate hexose monophosphates. The activity change of UDP-D-glucose 4-epimerase in tissue culture cells during the growth was in accord with changes in D-galactose content in cell walls and indicated the possibility to regulate the formation of polysaccharides containing D-galactose at the level of production of UDP-D-galactose in cells.

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