scholarly journals Effect of Plant Hormones on Tobacco Mosaic Virus Concentration in Tobacco Tissue Culture

10.5109/23667 ◽  
1978 ◽  
Vol 22 (4) ◽  
pp. 211-219
Author(s):  
Toshihiro Omura ◽  
Satoshi Wakimoto
Keyword(s):  
Tissue Culture ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Hormones ◽  
Virus Concentration ◽  
Tobacco Tissue Culture

scholarly journals Effect of Temperature and Zinc on Development of Tobacco-Mosaic Virus in Resistant and Susceptible Tomatoes

1969 ◽  
Vol 49 (1) ◽  
pp. 112-132
Author(s):  
Justo López García
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Effect Of Temperature ◽  
Late Winter ◽  
Virus Concentration ◽  
Buffer Solution ◽  
Tomato Plants ◽  
Foliar Sprays ◽  
Local Lesions ◽  
The Common

An investigation of the effects of temperature and of zinc on the development of tobacco-mosaic virus (TMV) in resistant and susceptible varieties of tomatoes was conducted under greenhouse conditions. The study included two experiments, one run during late winter and early spring, and the other during late spring and summer, 1963. Each experiment comprised four treatments and two temperatures. The tomato lines Hawaii 6832, OSU-8, and California 62 PM 22 were used as resistant varieties, whereas the OSU-435-4 line was used as the susceptible one. The common strain of tobacco-mosaic virus was used in this study. Zinc foliar sprays were applied to the tomato plants about 10 days before the seedlings were inoculated with TMV. Immediately after inoculation the plants were divided into two lots and moved to the two temperature houses (cool and hot). The virus concentration was determined by the local lesion assay. One gram of leaf tissue from the tomato plants was ground in a mortar with pestle and a 10-ml. buffer solution added. The extracted sap was squeezed through cheesecloth. One carborundum-dusted leaf of Necrotic Turk tobacco was inoculated. The local lesions were subsequently counted and used as a criterium. Increase in temperature tended to increase virus concentration, although the differences were not significant. Fresh weights of the tomato plants were found to be greater at lower temperatures. Zinc foliar sprays resulted in higher virus concentration and in production of an increased number of local lesions when the inoculum prepared from such treated plants was rubbed into Necrotic Turk tobacco leaves. Effects of zinc on fresh weights were not statistically significant. Zinc applied as foliar sprays appeared to be involved in virus synthesis and multiplication, with higher virus concentrations associated with the addition of zinc. The varieties Hawaii 6832 and OSU-8 were highly resistant, the variety California 62 PM 22 was heterozygous for resistance, and the variety OSU- 435-4 was highly susceptible to the common strain of TMV under the conditions prevailing in these experiments.

Die Bildung der infektiösen Ribonucleinsäure während der Vermehrung des Tabakmosaikvirus

1960 ◽  
Vol 15 (1) ◽  
pp. 38-45 ◽  
Author(s):  
Reto Engler ◽  
Gerhard Schramm
Keyword(s):  
Nucleic Acid ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Ribonucleic Acid ◽  
Virus Concentration ◽  
Free Virus ◽  
Direct Extraction ◽  
Nicotiana Glutinosa ◽  
General Hypothesis ◽  
Phenol Extraction

Young plants of Nicotiana tabacum (Var. Samsun) were infected with tobacco mosaic virus and kept at a constant temperature between 23 and 27°C and at constant illumination. The virus concentration was determined by bioassay on Nicotiana glutinosa. An exponential increase in virus concentration occurred 20-30 hours after infection. This latent period is significantly shorter after infection with virus ribonucleic acid. Probably the nucleic acid has to be liberated from the nucleoprotein before multiplication can start. The formation and multiplication of free virus ribonucleic acid could be demonstrated earlier than the formation of the complete virus. Infectious nucleic acid was measured by direct extraction of the plants with phenol. Nucleic acid included in the virus was determined after degradation of the free ribonucleic acid by incubation at 37°C and subsequent phenol extraction. The amount of free ribonucleic acid reaches a maximum 40 hours after infection and decreases afterwards to the extent as the virus bound ribonucleic acid increases. A general hypothesis for the biosynthesis of tobacco mosaic virus is given.

scholarly journals SOME CONDITIONS AFFECTING THE INTRACELLULAR ARRANGEMENT AND CONCENTRATION OF TOBACCO MOSAIC VIRUS PARTICLES IN LOCAL LESIONS

1967 ◽  
Vol 35 (1) ◽  
pp. 183-192 ◽  
Author(s):  
M. Weintraub ◽  
H. W. Ragetli ◽  
V. T. John
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Air Temperature ◽  
Systemic Infection ◽  
Fold Increase ◽  
Virus Concentration ◽  
Chenopodium Amaranticolor ◽  
Nicotiana Glutinosa ◽  
Virus Particles ◽  
Local Lesions

Tobacco mosaic virus particles were found in small packets and in small numbers, with the electron microscope, in necrotic leaf cells of Nicotiana glutinosa when the samples were fixed in glutaraldehyde and postfixed in OsO4, and the sections were stained with heavy metals. The numbers and size of the virus packets were increased greatly when the leaves were detached from the plant after inoculation Assay of concentration showed that detachment resulted in a 30-fold increase of virus. A similar increase in the number of virus particles detected by electron microscopy was produced by keeping inoculated plants at an air temperature of 26°C. A still greater increase in concentration was effected by incubating detached inoculated leaves at 26°C. Moreover the arrangement of virus particles in these cells resembled that of a systemic virus infection. Cells in local lesions of Chenopodium amaranticolor contained large numbers of virus particles both as packets and in the loose arrangement characteristic of systemic infection. Neither the number of particles nor their arrangement was affected in this host by detaching the leaf or by changing the air temperature. It is suggested that there may be two types of localized virus infections, one of which produces virus in low concentration and is amenable to changes in virus concentration and arrangement as a result of environmental manipulation.

scholarly journals STUDIES ON DISEASE RESISTANCE I. A TOBACCO RESISTANT TO ORDINARY TOBACCO MOSAIC

1969 ◽  
Vol 19 (1) ◽  
pp. 29-128
Author(s):  
J. A. B. Nolla
Keyword(s):  
Disease Resistance ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Concentration ◽  
Mild Form ◽  
Stages Of Growth ◽  
Quantitative Studies ◽  
Type 3 ◽  
Ring Spot

1. Infection of the resistant tobacco variety Ambalema occurs at all ages. 2. Symptoms in resistant plants ordinarily consist of clearing of the veins followed by small chlorotic interveinal areas of a mild type. 3. Infection occurs and mild or severe symptoms appear in transplants when these are inoculated at time of pulling. These plants later recover in the field, but the virus is always contained in their tissues. 4. Infection of Ambalema plants was determined by inoculation of the juices extracted from those plants into N. glutinosa and Havana No. 38 tobacco. 5. Ambalema tobacco is also resistant to yellow tobacco mosaic, and celery mosaic. It should not be forgotten that symptoms are produced in inoculated plants but the effects produced by the disease are not very significant. 6. By adequate quantitative studies it was determined that the Ambalema tobacco is significantly more resistant to ordinary tobacco mosaic than Havana No. 38 tobacco at all stages of growth. 7. Ambalema tobacco is very susceptible to cucumber mosaic, yellow cucumber mosaic, potato ring-spot, Wingard's tobacco ringspot and spot-necrosis, and somewhat less susceptible to the mottle and veinbanding viruses. 8. The virus concentration was found to be lower in inoculated resistant plants nine weeks afte r inoculation (13 weeks old) than five weeks after inoculation (9 weeks old). 9. A mild form of mosaic was isolated from resistant plants inoculated with the ordinary tobacco-mosaic virus. In its properties this mosaic virus seems similar to the ordinary tobacco-mosaic virus. This may possibly be regarded as a case of attenuation.

Sign in / Sign up

Export Citation Format

Share Document