scholarly journals Indole-3-acetic Acid in Douglas Fir

1976 ◽  
Vol 58 (3) ◽  
pp. 299-303 ◽  
Author(s):  
David R. Deyoe ◽  
Joe B. Zaerr
1978 ◽  
Vol 62 (6) ◽  
pp. 841-845 ◽  
Author(s):  
John L. Caruso ◽  
Ronald G. Smith ◽  
Lawrence M. Smith ◽  
Tsai-Ying Cheng ◽  
G. Doyle Daves

1976 ◽  
Vol 6 (3) ◽  
pp. 429-435 ◽  
Author(s):  
David R. Deyoe ◽  
Joe B. Zaerr

Comparison of extraction by diethyl ether solvent and by centrifugation into 20% methanol has revealed that the latter method was more effective in removing indole-3-acetic acid (IAA) from terminal and primary lateral shoots of Douglas fir (Pseudotsugamenziesii (Mirb.) Franco). The quantity of endogenous IAA extracted by centrifugation was 1.38 mg/kg and 2.36 mg/kg fresh weight for spring 1972 and spring 1971 collections, respectively, compared with 0.68 mg/kg and 0.72 mg/kg fresh weight acquired by the classic method of solvent extraction in diethyl ether. Centrifugation was rapid and provided an extract essentially free of pigments and interfering plant constituents, which permitted more efficient preparation of extracts for analysis by gas–liquid chromatography and mass spectrometry. The quantity of IAA present and its seasonal fluctuation in Douglas fir were verified.


1983 ◽  
Vol 22 (2) ◽  
pp. 589-590 ◽  
Author(s):  
John L. Caruso ◽  
Charles S. Zeisler

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