An improved method for extraction of indole-3-acetic acid from shoots of Douglas fir

1976 ◽  
Vol 6 (3) ◽  
pp. 429-435 ◽  
Author(s):  
David R. Deyoe ◽  
Joe B. Zaerr
Keyword(s):  
Acetic Acid ◽  
Diethyl Ether ◽  
Seasonal Fluctuation ◽  
Douglas Fir ◽  
Gas Liquid Chromatography ◽  
Fresh Weight ◽  
Lateral Shoots ◽  
Plant Constituents ◽  
Indole 3 Acetic Acid ◽  
Primary Lateral

Comparison of extraction by diethyl ether solvent and by centrifugation into 20% methanol has revealed that the latter method was more effective in removing indole-3-acetic acid (IAA) from terminal and primary lateral shoots of Douglas fir (Pseudotsugamenziesii (Mirb.) Franco). The quantity of endogenous IAA extracted by centrifugation was 1.38 mg/kg and 2.36 mg/kg fresh weight for spring 1972 and spring 1971 collections, respectively, compared with 0.68 mg/kg and 0.72 mg/kg fresh weight acquired by the classic method of solvent extraction in diethyl ether. Centrifugation was rapid and provided an extract essentially free of pigments and interfering plant constituents, which permitted more efficient preparation of extracts for analysis by gas–liquid chromatography and mass spectrometry. The quantity of IAA present and its seasonal fluctuation in Douglas fir were verified.

Reduction of Glyphosate-Induced Tillering in Sorghum (Sorghum bicolor) by Several Chemicals

Weed Science ◽  
1979 ◽  
Vol 27 (1) ◽  
pp. 69-73 ◽  
Author(s):  
J. R. Baur
Keyword(s):  
Acetic Acid ◽  
Sorghum Bicolor ◽  
Fresh Weight ◽  
True Leaf ◽  
Combination Treatments ◽  
Isopentenyl Adenine ◽  
Sublethal Doses ◽  
Indole 3 Acetic Acid

Sublethal doses of glyphosate [N-(phosphonomethyl)glycine] applied in a 50-μl drop in the partially furled third true leaf of 30-day-old sorghum [Sorghum bicolor(L.) Moench ‘Tophand’] seedlings induced basal stem swelling and bud release. Combining glyphosate with cycloheximide {3-[2-(3,5-dimethyl-2-oxycyclohexyl)-2-hydroxyethyl]glutarimide}, a cytokinin [N6(2-isopentenyl)adenine], or L-phenylalanine significantly reduced the incidence of basal stem swelling. No such reductions was observed when indole-3-acetic acid or L-tyrosine was combined with glyphosate. The fresh weights of the seedlings given the combination treatments reflected the effects of glyphosate, except one combination that included tyrosine significantly reduced fresh weight and three combinations that included phenylalanine significantly increased fresh weight.

Effect of Pseudomonas aeruginosa ZGKD2 on the Growth of Amorpha fruticosa L in Coal Gangue

2013 ◽  
Vol 807-809 ◽  
pp. 2023-2026
Author(s):  
Yu Xiu Zhang ◽  
Pei Li Shi ◽  
Qian Zhang
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Acetic Acid ◽  
Plant Growth ◽  
Root Length ◽  
Phosphate Solubilization ◽  
Fresh Weight ◽  
Amorpha Fruticosa ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

The cadmium-resistant Pseudomonas aeruginosa strain ZGKD2 was isolated from gangue pile of coal area. Production of siderophores, indole-3-acetic acid (IAA) and the solubilization of phosphate were observed in the strain. Two types of siderophores were identified by UV spectrophotometer. The highest production of IAA and phosphate solubilization were 2.0 ug/mL and 7.2 ug/mL. The root length, plant height and fresh weight of Amorpha fruticosa L in the substrates of Coal gannue and losses were promoted after inoculation with ZGKD2. These data indicated that Pseudomonas aeruginosa strain ZGKD2 was a plant growth-promoting bacterial (PGPB).

scholarly journals Indole-3-acetic Acid in Douglas Fir

1976 ◽  
Vol 58 (3) ◽  
pp. 299-303 ◽  
Author(s):  
David R. Deyoe ◽  
Joe B. Zaerr
Keyword(s):  
Acetic Acid ◽  
Douglas Fir ◽  
Indole 3 Acetic Acid

Indolic Structure Metabolites as Potential Biomarkers of Non-Infection Diseases

2020 ◽  
Vol 26 ◽  
Author(s):  
Natalia V. Beloborodova ◽  
Ekaterina A. Chernevskaya ◽  
Maria L. Getsina
Keyword(s):  
Acetic Acid ◽  
Propionic Acid ◽  
Experimental Studies ◽  
Indoxyl Sulfate ◽  
Cochrane Library ◽  
Comprehensive Treatment ◽  
Gas Liquid Chromatography ◽  
Tryptophan Metabolites ◽  
Concentration Changes ◽  
Indole 3 Acetic Acid

: Interest in indolic structure metabolites including a number of products of microbial biotransformation of the aromatic amino acid tryptophan is increasingly growing. The review prepared by a team of authors is based on a close scrutiny of data available in PubMed, Scopus, Cyberleninka, Clinical Trials, Cochrane Library, eventually narrowing the search to a set of keywords as tryptophan metabolites; plasma metabolomics profiling; metabolomics fingerprinting; gas-, liquid chromatography mass spectrometry; serotonin; melatonin; tryptamine; indoxyl sulfate; indole-3-acetic acid; indole-3-propionic acid; 5-hydroxyindoleacetic acid; gut microbiota; microbial metabolites. It resulted in a summary that outlines the pattern of changes in the level of indolic structure metabolites in a number of diseases and deals with the data from the field of human microbiota metabolites. In modern experimental studies, including the use of gnotobiological (germ-free) animals, it has been convincingly proved that the formation of tryptophan metabolites such as indole-3-acetic acid, indole-3-propionic acid, tryptamine, and indoxyl sulfate is associated with gut bacteria. Attention to some concentration changes of indolic compounds is due to the fact that pronounced deviations or a significant decrease of these metabolites in the blood were found in a number of problematic cardiovascular, brain or gastrointestinal diseases. The literature-based analysis allowed the authors to conclude that a constant (normal) level of the main metabolites of the indolic structure in the human body is maintained by a limited list of strict anaerobic bacteria from the gut of a healthy body belonging to the species of Clostridium, Bacteroides, Peptostreptococcus, Eubacteria, etc. The authors focus on several metabolites of the indolic structure that can be called clinically significant in certain diseases, such as schizophrenia, depression, atherosclerosis, colorectal cancer, etc. Determining the level of indole metabolites in the blood can be used to diagnose and monitor the effectiveness of a comprehensive treatment approach.

scholarly journals Determination of Indole-3-acetic Acid in Douglas Fir Using a Deuterated Analog and Selected Ion Monitoring

1978 ◽  
Vol 62 (6) ◽  
pp. 841-845 ◽  
Author(s):  
John L. Caruso ◽  
Ronald G. Smith ◽  
Lawrence M. Smith ◽  
Tsai-Ying Cheng ◽  
G. Doyle Daves
Keyword(s):  
Acetic Acid ◽  
Douglas Fir ◽  
Selected Ion Monitoring ◽  
Deuterated Analog ◽  
Indole 3 Acetic Acid

Indole-3-acetic acid in Douglas fir seedlings: A reappraisal

1983 ◽  
Vol 22 (2) ◽  
pp. 589-590 ◽  
Author(s):  
John L. Caruso ◽  
Charles S. Zeisler
Keyword(s):  
Acetic Acid ◽  
Douglas Fir ◽  
Indole 3 Acetic Acid
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