Table 1. Performance comparison of 3D fluorescence microscopy in the context of long-term in vivo imaging of entire embryos

2010 ◽  
Vol 2010 (5) ◽  
pp. pdb.tab1top78-pdb.tab1top78
Keyword(s):  
Fluorescence Microscopy ◽  
In Vivo Imaging ◽  
Performance Comparison ◽  
3D Fluorescence Microscopy

Long-Term In Vivo Imaging of Individual Microglial Cells

2019 ◽  
pp. 177-189
Author(s):  
Angelos A. Skodras ◽  
Jasmin K. Hefendehl ◽  
Jonas J. Neher
Keyword(s):  
In Vivo Imaging ◽  
Microglial Cells

Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex

Nature ◽  
2002 ◽  
Vol 420 (6917) ◽  
pp. 788-794 ◽  
Author(s):  
Joshua T. Trachtenberg ◽  
Brian E. Chen ◽  
Graham W. Knott ◽  
Guoping Feng ◽  
Joshua R. Sanes ◽  
...  
Keyword(s):  
Synaptic Plasticity ◽  
In Vivo Imaging

In vivo imaging of hepatic excretory function in the rat by fluorescence microscopy

2012 ◽  
Vol 5 (7) ◽  
pp. 571-581 ◽  
Author(s):  
Peter Recknagel ◽  
Ralf A. Claus ◽  
Ute Neugebauer ◽  
Michael Bauer ◽  
Falk A. Gonnert
Keyword(s):  
Fluorescence Microscopy ◽  
In Vivo Imaging ◽  
Excretory Function

In vivo imaging of myocardial cell death using a peptide probe and assessment of long-term heart function

2013 ◽  
Vol 172 (1) ◽  
pp. 367-373 ◽  
Author(s):  
Bodhraj Acharya ◽  
Kai Wang ◽  
In-San Kim ◽  
WoongChol Kang ◽  
Chanil Moon ◽  
...  
Keyword(s):  
Cell Death ◽  
In Vivo Imaging ◽  
Heart Function ◽  
Myocardial Cell ◽  
Peptide Probe

scholarly journals Long-term in vivo time-lapse imaging of synapse development and plasticity in the cerebellum

2014 ◽  
Vol 111 (1) ◽  
pp. 208-216 ◽  
Author(s):  
Naoko Nishiyama ◽  
Jeremy Colonna ◽  
Elise Shen ◽  
Jennifer Carrillo ◽  
Hiroshi Nishiyama
Keyword(s):  
In Vivo Imaging ◽  
Time Window ◽  
Time Lapse ◽  
Mammalian Brain ◽  
Cerebellar Neurons ◽  
Brain Functions ◽  
Time Lapse Imaging ◽  
Synaptic Circuitry

Synapses are continuously formed and eliminated throughout life in the mammalian brain, and emerging evidence suggests that this structural plasticity underlies experience-dependent changes of brain functions such as learning and long-term memory formation. However, it is generally difficult to understand how the rewiring of synaptic circuitry observed in vivo eventually relates to changes in animal's behavior. This is because afferent/efferent connections and local synaptic circuitries are very complicated in most brain regions, hence it is largely unclear how sensorimotor information is conveyed, integrated, and processed through a brain region that is imaged. The cerebellar cortex provides a particularly useful model to challenge this problem because of its simple and well-defined synaptic circuitry. However, owing to the technical difficulty of chronic in vivo imaging in the cerebellum, it remains unclear how cerebellar neurons dynamically change their structures over a long period of time. Here, we showed that the commonly used method for neocortical in vivo imaging was not ideal for long-term imaging of cerebellar neurons, but simple optimization of the procedure significantly improved the success rate and the maximum time window of chronic imaging. The optimized method can be used in both neonatal and adult mice and allows time-lapse imaging of cerebellar neurons for more than 5 mo in ∼80% of animals. This method allows vital observation of dynamic cellular processes such as developmental refinement of synaptic circuitry as well as long-term changes of neuronal structures in adult cerebellum under longitudinal behavioral manipulations.

scholarly journals Long-term in vivo imaging of translated RNAs for gene therapy

Gene Therapy ◽  
2014 ◽  
Vol 21 (4) ◽  
pp. 434-439 ◽  
Author(s):  
K Pinel ◽  
J Lacoste ◽  
G Plane ◽  
M Ventura ◽  
F Couillaud
Keyword(s):  
Gene Therapy ◽  
In Vivo Imaging

scholarly journals A general method to fine-tune fluorophores for live-cell and in vivo imaging

2017 ◽  
Author(s):  
Jonathan B. Grimm ◽  
Anand K. Muthusamy ◽  
Yajie Liang ◽  
Timothy A. Brown ◽  
William C. Lemon ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
In Vivo Imaging ◽  
Chemical Properties ◽  
Live Cell ◽  
Live Cells ◽  
Fine Tune ◽  
General Method ◽  
And Chemical Properties

ABSTRACTPushing the frontier of fluorescence microscopy requires the design of enhanced fluorophores with finely tuned properties. We recently discovered that incorporation of four-membered azetidine rings into classic fluorophore structures elicits substantial increases in brightness and photostability, resulting in the ‘Janelia Fluor’ (JF) series of dyes. Here, we refine and extend this strategy, showing that incorporation of 3-substituted azetidine groups allows rational tuning of the spectral and chemical properties with unprecedented precision. This strategy yields a palette of new fluorescent and fluorogenic labels with excitation ranging from blue to the far-red with utility in live cells, tissue, and animals.

scholarly journals Advanced fluorescence microscopy for in vivo imaging of neuronal activity

Optica ◽  
2019 ◽  
Vol 6 (6) ◽  
pp. 758 ◽  
Author(s):  
Giuseppe Sancataldo ◽  
Ludovico Silvestri ◽  
Anna Letizia Allegra Mascaro ◽  
Leonardo Sacconi ◽  
Francesco Saverio Pavone
Keyword(s):  
Fluorescence Microscopy ◽  
Neuronal Activity ◽  
In Vivo Imaging

scholarly journals In vivo live imaging of postnatal neural stem cells

Development ◽  
2021 ◽  
Vol 148 (18) ◽  
Author(s):  
Alina Marymonchyk ◽  
Sarah Malvaut ◽  
Armen Saghatelyan
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
In Vivo Imaging ◽  
Live Imaging ◽  
Future Directions ◽  
Environmental Signals ◽  
New Information ◽  
Imaging Results

ABSTRACT Neural stem cells (NSCs) are maintained in specific regions of the postnatal brain and contribute to its structural and functional plasticity. However, the long-term renewal potential of NSCs and their mode of division remain elusive. The use of advanced in vivo live imaging approaches may expand our knowledge of NSC physiology and provide new information for cell replacement therapies. In this Review, we discuss the in vivo imaging methods used to study NSC dynamics and recent live-imaging results with respect to specific intracellular pathways that allow NSCs to integrate and decode different micro-environmental signals. Lastly, we discuss future directions that may provide answers to unresolved questions regarding NSC physiology.

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