In vivo imaging of hepatic excretory function in the rat by fluorescence microscopy

2012 ◽  
Vol 5 (7) ◽  
pp. 571-581 ◽  
Author(s):  
Peter Recknagel ◽  
Ralf A. Claus ◽  
Ute Neugebauer ◽  
Michael Bauer ◽  
Falk A. Gonnert
Keyword(s):  
Fluorescence Microscopy ◽  
In Vivo Imaging ◽  
Excretory Function

scholarly journals A general method to fine-tune fluorophores for live-cell and in vivo imaging

2017 ◽  
Author(s):  
Jonathan B. Grimm ◽  
Anand K. Muthusamy ◽  
Yajie Liang ◽  
Timothy A. Brown ◽  
William C. Lemon ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
In Vivo Imaging ◽  
Chemical Properties ◽  
Live Cell ◽  
Live Cells ◽  
Fine Tune ◽  
General Method ◽  
And Chemical Properties

ABSTRACTPushing the frontier of fluorescence microscopy requires the design of enhanced fluorophores with finely tuned properties. We recently discovered that incorporation of four-membered azetidine rings into classic fluorophore structures elicits substantial increases in brightness and photostability, resulting in the ‘Janelia Fluor’ (JF) series of dyes. Here, we refine and extend this strategy, showing that incorporation of 3-substituted azetidine groups allows rational tuning of the spectral and chemical properties with unprecedented precision. This strategy yields a palette of new fluorescent and fluorogenic labels with excitation ranging from blue to the far-red with utility in live cells, tissue, and animals.

scholarly journals Advanced fluorescence microscopy for in vivo imaging of neuronal activity

Optica ◽  
2019 ◽  
Vol 6 (6) ◽  
pp. 758 ◽  
Author(s):  
Giuseppe Sancataldo ◽  
Ludovico Silvestri ◽  
Anna Letizia Allegra Mascaro ◽  
Leonardo Sacconi ◽  
Francesco Saverio Pavone
Keyword(s):  
Fluorescence Microscopy ◽  
Neuronal Activity ◽  
In Vivo Imaging

Abstract 28: Serial in Vivo Imaging of Thrombus Inflammation Predicts Venous Thrombus Resolution

2012 ◽  
Vol 32 (suppl_1) ◽  
Author(s):  
Chase W Kessinger ◽  
Jason R McCarthy ◽  
Charles P Lin ◽  
Peter K Henke ◽  
Farouc A Jaffer
Keyword(s):  
Fluorescence Microscopy ◽  
In Vivo Imaging ◽  
Macrophage Infiltration ◽  
Intravital Fluorescence Microscopy ◽  
Targeted Nanoparticles ◽  
Macrophage Activity ◽  
Vein Thrombosis ◽  
Deep Vein ◽  
In Vivo Assessment

Introduction: Inflammation following deep vein thrombosis (DVT) critically modulates thrombus resolution. However it is unknown whether the degree of inflammation in vivo predicts the magnitude of thrombus resolution. Here, we utilize serial multichannel intravital fluorescence microscopy (IVFM) to spatially map and quantify macrophage infiltration in thrombus, and then to determine the degree of thrombus resolution in vivo . Methods: C57Bl/6 mice (n=5) underwent topical ferric chloride injury to induce femoral venous thrombosis. On day 3, mice were i.v. injected with macrophage-targeted nanoparticles (CLIO-AF555, ex/em 555/565nm). On day 4, survival IVFM of thrombus macrophages was performed. In addition, venography was concomitantly performed (pre-injection of FITC-dextran 45 minutes before IVFM, MW 2000 kD, 490/520nm) to quantify DVT area and length in vivo at day 4 and 6. IVFM image analysis was performed using ImageJ. Mid-luminal z-stacks (40 μm thickness) were analyzed. Thrombus ROIs were outlined using FITC-dextran generated angiograms, and used calculating macrophage target-to-background ratios (TBR) and thrombus area and length. Contralateral sham femoral veins were imaged as controls. Imaging was followed by histopathology and fluorescence microscopy of the cryosectioned tissue. Results: At day 4, the baseline femoral DVT area and length were 0.22±0.08mm 2 and 1.29±0.16mm (mean±sd). The day 4 macrophage activity was noted to be heterogeneous with peripheral infiltration of the thrombus, and occasionally with some deeper penetration into the thrombus interior. At day 6, the thrombus area and length were reduced compared to day 4 (p<0.005 for both). The day 4 thrombus macrophage TBR significantly predicted the degree of thrombus reduction (correlation of initial macrophage TBR and Δthrombus area, r=0.97, p=0.001). Conclusion: This serial in vivo assessment of murine DVT resolution demonstrates that degree of macrophage infiltration at day 4 predicts the magnitude of thrombus resolution at day 6. Molecular imaging of DVT inflammation in vivo could provide new insights into DVT resolution and the development of the post-thrombotic syndrome, and also in evaluating inflammatory-modulating therapies for improved DVT resolution.

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