scholarly journals A general method to fine-tune fluorophores for live-cell and in vivo imaging

2017 ◽  
Author(s):  
Jonathan B. Grimm ◽  
Anand K. Muthusamy ◽  
Yajie Liang ◽  
Timothy A. Brown ◽  
William C. Lemon ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
In Vivo Imaging ◽  
Chemical Properties ◽  
Live Cell ◽  
Live Cells ◽  
Fine Tune ◽  
General Method ◽  
And Chemical Properties

ABSTRACTPushing the frontier of fluorescence microscopy requires the design of enhanced fluorophores with finely tuned properties. We recently discovered that incorporation of four-membered azetidine rings into classic fluorophore structures elicits substantial increases in brightness and photostability, resulting in the ‘Janelia Fluor’ (JF) series of dyes. Here, we refine and extend this strategy, showing that incorporation of 3-substituted azetidine groups allows rational tuning of the spectral and chemical properties with unprecedented precision. This strategy yields a palette of new fluorescent and fluorogenic labels with excitation ranging from blue to the far-red with utility in live cells, tissue, and animals.

scholarly journals A general method to fine-tune fluorophores for live-cell and in vivo imaging

2017 ◽  
Vol 14 (10) ◽  
pp. 987-994 ◽  
Author(s):  
Jonathan B Grimm ◽  
Anand K Muthusamy ◽  
Yajie Liang ◽  
Timothy A Brown ◽  
William C Lemon ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Live Cell ◽  
Fine Tune ◽  
General Method

scholarly journals Insights in Behavior of Variably Formulated Alginate-Based Microcapsules for Cell Transplantation

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Pia Montanucci ◽  
Silvia Terenzi ◽  
Claudio Santi ◽  
Ilaria Pennoni ◽  
Vittorio Bini ◽  
...  
Keyword(s):  
Divalent Cations ◽  
Chemical Properties ◽  
Live Cells ◽  
High Performing ◽  
Physical Chemical ◽  
Degenerative Disorders ◽  
Selection Of ◽  
Better Than

Alginate-based microencapsulation of live cells may offer the opportunity to treat chronic and degenerative disorders. So far, a thorough assessment of physical-chemical behavior of alginate-based microbeads remains cloudy. A disputed issue is which divalent cation to choose for a high performing alginate gelling process. Having selected, in our system, high mannuronic (M) enriched alginates, we studied different gelling cations and their combinations to determine their eventual influence on physical-chemical properties of the final microcapsules preparation,in vitroandin vivo. We have shown that used of ultrapure alginate allows for high biocompatibility of the formed microcapsules, regardless of gelation agents, while use of different gelling cations is associated with corresponding variable effects on the capsules’ basic architecture, as originally reported in this work. However, only the final application which the capsules are destined to will ultimately guide the selection of the ideal, specific gelling divalent cations, since in principle there are no capsules that are better than others.

Ligand-displacement-based two-photon fluorogenic probe for visualizing mercapto biomolecules in live cells, Drosophila brains and zebrafish

The Analyst ◽  
2018 ◽  
Vol 143 (14) ◽  
pp. 3433-3441 ◽  
Author(s):  
Yanfei Zhao ◽  
Yun Ni ◽  
Liulin Wang ◽  
Chenchen Xu ◽  
Chenqi Xin ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Live Cell ◽  
Live Cells ◽  
Two Photon ◽  
Ligand Displacement ◽  
Cell Tissue ◽  
Fluorogenic Probe ◽  
Displacement Based

We report the Fe(iii)-based complex TPFeS which acts as a novel ligand-displacement-based TP fluorogenic probe for the rapid detection of mercapto biomolecules both in vitro and in live cell/tissue/in vivo imaging.

Impact of solid-state photochemical [2+2] cycloaddition on coordination polymers for diverse applications

2020 ◽  
Vol 49 (28) ◽  
pp. 9556-9563 ◽  
Author(s):  
Samim Khan ◽  
Basudeb Dutta ◽  
Mohammad Hedayetullah Mir
Keyword(s):  
Solid State ◽  
Coordination Polymers ◽  
Chemical Properties ◽  
Physical And Chemical Properties ◽  
Physical And Chemical ◽  
Fine Tune ◽  
And Chemical Properties

This Frontier article highlights the advancement of [2+2] photocycloaddition reactions within coordination polymers to fine tune their diverse physical and chemical properties.

scholarly journals Live Cell in Vitro and in Vivo Imaging Applications: Accelerating Drug Discovery

Pharmaceutics ◽  
2011 ◽  
Vol 3 (2) ◽  
pp. 141-170 ◽  
Author(s):  
Beverley Isherwood ◽  
Paul Timpson ◽  
Ewan J McGhee ◽  
Kurt I Anderson ◽  
Marta Canel ◽  
...  
Keyword(s):  
Drug Discovery ◽  
In Vivo Imaging ◽  
Live Cell

In vivo imaging of hepatic excretory function in the rat by fluorescence microscopy

2012 ◽  
Vol 5 (7) ◽  
pp. 571-581 ◽  
Author(s):  
Peter Recknagel ◽  
Ralf A. Claus ◽  
Ute Neugebauer ◽  
Michael Bauer ◽  
Falk A. Gonnert
Keyword(s):  
Fluorescence Microscopy ◽  
In Vivo Imaging ◽  
Excretory Function

scholarly journals PinMol: Python application for designing molecular beacons for live cell imaging of endogenous mRNAs

2018 ◽  
Author(s):  
Livia V. Bayer ◽  
Omar S. Omar ◽  
Diana P. Bratu ◽  
Irina E. Catrina
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Molecular Beacon ◽  
Structural Information ◽  
Live Cell ◽  
Molecular Beacons ◽  
Intramolecular Interactions ◽  
Live Cells ◽  
Target Rna

ABSTRACTMolecular beacons are nucleic acid oligomers labeled with a fluorophore and a quencher that fold in a hairpin-shaped structure, which fluoresce only when bound to their target RNA. They are used for the visualization of endogenous mRNAs in live cells. Here, we report a Python program (PinMol) that designs molecular beacons best suited for live cell imaging by using structural information from secondary structures of the target RNA, predicted via energy minimization approaches. PinMol takes into account the accessibility of the targeted regions, as well as the inter- and intramolecular interactions of each selected probe. To demonstrate its applicability, we synthesized an oskar mRNA-specific molecular beacon (osk1236), which is selected by PinMol to target a more accessible region than a manually designed oskar-specific molecular beacon (osk2216). We previously demonstrated osk2216 to be efficient in detecting oskar mRNA in in vivo experiments. Here, we show that osk1236 outperformed osk2216 in live cell imaging experiments.

scholarly journals Advanced fluorescence microscopy for in vivo imaging of neuronal activity

Optica ◽  
2019 ◽  
Vol 6 (6) ◽  
pp. 758 ◽  
Author(s):  
Giuseppe Sancataldo ◽  
Ludovico Silvestri ◽  
Anna Letizia Allegra Mascaro ◽  
Leonardo Sacconi ◽  
Francesco Saverio Pavone
Keyword(s):  
Fluorescence Microscopy ◽  
Neuronal Activity ◽  
In Vivo Imaging
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