scholarly journals DNA-mediated self-assembly of gold nanoparticles on protein superhelix

2018 ◽  
Author(s):  
Tao Zhang ◽  
Ingemar André
Keyword(s):  
Gold Nanoparticles ◽  
Self Assembly ◽  
De Novo ◽  
Specific Binding ◽  
Protein Structures ◽  
Spatial Arrangement ◽  
Inorganic Nanoparticles ◽  
Covalent Attachment ◽  
Site Specific ◽  
Conjugation Method

AbstractRecent advances in protein engineering have enabled methods to control the self-assembly of protein on various length-scales. One attractive application for designed proteins is to direct the spatial arrangement of nanomaterials of interest. Until now, however, a reliable conjugation method is missing to facilitate site-specific positioning. In particular, bare inorganic nanoparticles tend to aggregate in the presence of buffer conditions that are often required for the formation of stable proteins. Here, we demonstrated a DNA mediated conjugation method to link gold nanoparticles with protein structures. To achieve this, we constructed de novo designed protein fibers based on previously published uniform alpha-helical units. DNA modification rendered gold nanoparticles with increased stability against ionic solutions and the use of complementary strands hybridization guaranteed the site-specific binding to the protein. The combination of high resolution placement of anchor points in designed protein assemblies with the increased control of covalent attachment through DNA binding can enable investigations of multilevel physical coupling events of nanocomponents on protein templates and expand the application of protein structures to material sciences.

scholarly journals Synthesis and Applications of Porphyrin-Biomacromolecule Conjugates

2021 ◽  
Vol 9 ◽  
Author(s):  
Pravin Pathak ◽  
Mohammad Amin Zarandi ◽  
Xiao Zhou ◽  
Janarthanan Jayawickramarajah
Keyword(s):  
Nucleic Acid ◽  
Functional Groups ◽  
Self Assembly ◽  
De Novo ◽  
Solid Phase ◽  
Academic Research ◽  
Fine Tuning ◽  
Cancer Tissue ◽  
Site Specific ◽  
Synthetic Approaches

With potential applications in materials and especially in light-responsive biomedicine that targets cancer tissue selectively, much research has focused on developing covalent conjugation techniques to tether porphyrinoid units to various biomacromolecules. This review details the key synthetic approaches that have been employed in the recent decades to conjugate porphyrinoids with oligonucleotides and peptides/proteins. In addition, we provide succinct discussions on the subsequent applications of such hybrid systems and also give a brief overview of the rapidly progressing field of porphyrin-antibody conjugates. Since nucleic acid and peptide systems vary in structure, connectivity, functional group availability and placement, as well as stability and solubility, tailored synthetic approaches are needed for conjugating to each of these biomacromolecule types. In terms of tethering to ONs, porphyrins are typically attached by employing bioorthogonal chemistry (e.g., using phosphoramidites) that drive solid-phase ON synthesis or by conducting post-synthesis modifications and subsequent reactions (such as amide couplings, hydrazide-carbonyl reactions, and click chemistry). In contrast, peptides and proteins are typically conjugated to porphyrinoids using their native functional groups, especially the thiol and amine side chains. However, bioorthogonal reactions (e.g., Staudinger ligations, and copper or strain promoted alkyne-azide cycloadditions) that utilize de novo introduced functional groups onto peptides/proteins have seen vigorous development, especially for site-specific peptide-porphyrin tethering. While the ON-porphyrin conjugates have largely been explored for programmed nanostructure self-assembly and artificial light-harvesting applications, there are some reports of ON-porphyrin systems targeting clinically translational applications (e.g., antimicrobial biomaterials and site-specific nucleic acid cleavage). Conjugates of porphyrins with proteinaceous moieties, on the other hand, have been predominantly used for therapeutic and diagnostic applications (especially in photodynamic therapy, photodynamic antimicrobial chemotherapy, and photothermal therapy). The advancement of the field of porphyrinoid-bioconjugation chemistry from basic academic research to more clinically targeted applications require continuous fine-tuning in terms of synthetic strategies and hence there will continue to be much exciting work on porphyrinoid-biomacromolecule conjugation.

scholarly journals Regulation of Cu,Zn superoxide dismutase with copper. Caeruloplasmin maintains levels of functional enzyme activity during differentiation of K562 cells

1991 ◽  
Vol 274 (1) ◽  
pp. 153-158 ◽  
Author(s):  
S S Percival ◽  
E D Harris
Keyword(s):  
Superoxide Dismutase ◽  
De Novo ◽  
Specific Binding ◽  
K562 Cells ◽  
Sod Activity ◽  
Site Specific ◽  
Copper Protein ◽  
Mrna Concentration ◽  
Plasma Copper ◽  
Mnsod Activity

K562 cells, a human erythroleukaemic cell line blocked for differentiation, commit towards erythrocytes when exposed to haemin (20 microM). The cells synthesize fetal haemoglobins and show site-specific binding of caeruloplasmin, a plasma copper protein. These events are set into motion by haemin. On the assumption that the binding of caeruloplasmin could reflect a greater need for copper, we sought to determine whether the transfer of 67Cu from caeruloplasmin was accelerated in haemin-induced compared with non-induced K562 cells. Cu,Zn superoxide dismutase (CuZnSOD) was the recipient. Haemin induction caused the K562 cells to lose CuZnSOD activity. By 96 h, the level of SOD activity was less than 60% of that of non-induced cells. The loss was confined entirely to the CuZn form, MnSOD activity staying essentially unchanged. Although CuZnSOD activity declined with the haemin induction, the incorporation of [4,5-3H]lysine into immunoprecipitable CuZnSOD protein was unaffected. There was also no change in CuZnSOD mRNA concentration in haemin-induced cells. Thus a loss of enzyme did not correlate with a decline in the synthesis de novo of CuZnSOD protein. When 48 h-induced cells were transferred to a medium supplemented with 0.2 microM-caeruloplasmin, CuZnSOD activity was restored to control levels in 24 h. Caeruloplasmin also stimulated the incorporation of [3H]lysine into immunoprecipitable CuZnSOD protein. Caeruloplasmin addition may have affected a post-translational regulatory site for CuZnSOD biosynthesis, possibly by providing copper for the newly synthesized enzyme.

DNA ORIGAMI SITE-SPECIFIC ARRANGEMENT OF GOLD NANOPARTICLES

NANO ◽  
2013 ◽  
Vol 08 (06) ◽  
pp. 1350064
Author(s):  
AMOAKO GEORGE ◽  
RIAN YE ◽  
LIZHOU ZHUANG ◽  
XIAOHONG YANG ◽  
ZHIYONG SHEN ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gel Electrophoresis ◽  
Dna Origami ◽  
Covalent Attachment ◽  
Agarose Gel Electrophoresis ◽  
Dna Oligomers ◽  
Site Specific ◽  
Modified Dna ◽  
Vis Spectroscopy ◽  
Potential Use

Controlling matter at the nanoscale holds a lot of promise in nanotechnology. The DNA origami is promising if used as a template to design and arrange matter at the nanoscale. We have used the DNA origami approach to engineer staple strands at selected sites for attachment of gold nanoparticles. The covalent attachment of thiol-modified DNA oligomers was used to functionalize gold nanoparticles. These oligomers then hybridize with complementary strands extended on selected staple strands on the DNA origami surface with nanometer precision. Gold nanoparticles of 5 nm diameter were arranged across a DNA origami tube to form a C -shape which has potential use in electronics and plasmonics. Agarose gel electrophoresis, AFM, UV-Vis spectroscopy and TEM were used to characterize the structure.

scholarly journals Selective Detection of Human Lung Adenocarcinoma Cells Based on the Aptamer-Conjugated Self-Assembled Monolayer of Gold Nanoparticles

Micromachines ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 195 ◽  
Author(s):  
Ngoc-Viet Nguyen ◽  
Chun-Ping Jen
Keyword(s):  
Gold Nanoparticles ◽  
Self Assembly ◽  
Human Lung ◽  
Specific Binding ◽  
Physical And Mechanical Properties ◽  
Target Cells ◽  
Self Assembled Monolayer ◽  
Assembly Method ◽  
Human Lung Adenocarcinoma Cells ◽  
Self Assembled

This study established a microfluidic chip for the capture of A549 human lung circulating tumor cells via the aptamer-conjugated self-assembled monolayer (SAM) of gold nanoparticles (AuNPs) in the channel. AuNPs are among the most attractive nanomaterials for the signal enhancement of biosensors owing to their unique chemical, physical, and mechanical properties. The microchip was fabricated using soft photolithography and casting and molding techniques. A self-assembly method was designed to attach AuNPs, cell-specific aptamers, and target cells onto the desired area (i.e., SAM area). In this study, the gold microelectrode configuration was characterized by fluorescence microscopy and impedance measurements to confirm the important modification steps. Subsequently, several investigations with the proposed assay were conducted with different cell samples to determine the specific binding ability of the device for A549 adenocarcinoma cancer cells. This work has ensured a simple, convenient, selective, and sensitive approach for the development of biosensors for lung cancer detection during the early stages.

Flow hydrodynamics-dependent assembly of polymer-tethered gold nanoparticles in microfluidic channels

2020 ◽  
Vol 4 (11) ◽  
pp. 3240-3250
Author(s):  
Xi Mao ◽  
Zhengping Tan ◽  
Wei Lan ◽  
Huayang Wang ◽  
Haiying Tan ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Self Assembly ◽  
Inorganic Nanoparticles ◽  
Microfluidic Channels ◽  
Microfluidic Chips

Recently, self-assembly of polymer-tethered inorganic nanoparticles (NPs) in microfluidic chips has been employed as an effective approach to fabricate novel assemblies.

scholarly journals Gum Acacia Functionalized Colloidal Gold Nanoparticles of Letrozole as Biocompatible Drug Delivery Carrier for Treatment of Breast Cancer

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1554
Author(s):  
Hibah M. Aldawsari ◽  
Sima Singh ◽  
Nabil A. Alhakamy ◽  
Rana B. Bakhaidar ◽  
Abdulrahman A. Halwani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Postmenopausal Women ◽  
Specific Binding ◽  
Cancer Cell Line ◽  
Good Alternative ◽  
Inorganic Nanoparticles ◽  
Gum Acacia ◽  
Drug Delivery Carrier

The most prevalent malignancy among postmenopausal women is breast cancer. It is one of the leading causes of cancer-related mortality among women. Letrozole (LTZ) is a clinically approved inhibitor for breast cancer in postmenopausal women. However, due to poor aqueous solubility, non-specific binding, unwanted toxicity, and poor blood circulation hampered its clinical applications. To maximize the pharmacological effects and minimize the side effects, inorganic nanoparticles are a good alternative. Due to excellent biocompatibility and minimum cytotoxicity, gold nanoparticles (AuNPs) offer distinct benefits over other metal nanoparticles. Emerging as attractive components, AuNPs and Gum acacia (GA) have been extensively studied as biologically safe nanomaterials for the treatment of cancers. This study reports the synthesis and characterization of GA stabilized gold nanoparticles (GA-AuNPs) of LTZ for breast cancer treatment. The observed particle size of optimized LTZ @ GA-AuNPs was 81.81 ± 4.24 nm in size, 0.286 ± 0.143 of polydispersity index (PDI) and −14.6 ± −0.73 mV zeta potential. The biologically synthesized LTZ @ GA-AuNPs also demonstrated dose-dependent cytotoxicity against the human breast cancer cell line MCF-7, with an inhibitory concentration (IC50) of 3.217 ± 0.247. We determined the hemolytic properties of the LTZ @ GA-AuNPs to evaluate the interaction between the nanoparticles and blood components. Results showed that there is no interaction between LTZ @ GA-AuNPs and blood. In conclusion, the findings indicate that LTZ @ GA-AuNPs has significant potential as a promising drug delivery carrier for treating breast cancer in postmenopausal women.

A New Immunosensor for Serum Myeloperoxidase Based on Self-Assembly of Multi-Walled Carbon Nanotubes/Thionine/Gold Nanoparticles-Chitosan

2011 ◽  
Vol 343-344 ◽  
pp. 1207-1211 ◽  
Author(s):  
Qi Zhi Diao ◽  
Yuan Li ◽  
Mi Zhou ◽  
Guo Ming Xie
Keyword(s):  
Carbon Nanotubes ◽  
Gold Nanoparticles ◽  
Self Assembly ◽  
Specific Binding ◽  
Electrochemical Immunosensor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Current Signal ◽  
Multi Walled Carbon Nanotubes ◽  
Response Current ◽  
Walled Carbon Nanotubes

A new electrochemical immunosensor for serum myeloperoxidase (MPO) has been developed based on the self-assembly multilays of multi-walled carbon nanotubes (MWNTs), thionine (THI), gold nanoparticles (GNPs) and chitosanon (CHIT) on the glassy carbon electrode (GCE). The antibody of MPO (anti-MPO) was absorbed on the surface of GNPs monolayer. Horseradish peroxidase (HRP) was employed to block non-specific binding and amplify the response current signal. It was observed that the peak current was linear with the MPO concentration in a range of 2.5-125 µgl-1. The detection limit was 1.425 µgl-1 (S/N=3). Correlation analysis showed that this new immunosensor assay has a significant correlation with enzyme-linked immunosorbent assay (ELISA) (r=0.96, p>0.05) for 40 clinical specimens.

Self-assembly and characterization of transferrin–gold nanoconstructs and their interaction with bio-interfaces

Nanoscale ◽  
2015 ◽  
Vol 7 (17) ◽  
pp. 8062-8070 ◽  
Author(s):  
Birgitte H. McDonagh ◽  
Sondre Volden ◽  
Sina M. Lystvet ◽  
Gurvinder Singh ◽  
Marit-Helen G. Ese ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Optical Properties ◽  
Self Assembly ◽  
Specific Receptor ◽  
Receptor Targeting ◽  
Site Specific

Transferrin (Tf) conjugated to gold nanoparticles and clusters combine the protein's site-specific receptor targeting capabilities with the optical properties imparted by the nano-sized gold.

Higher order self-assembly of vesicles by site-specific binding

Science ◽  
1994 ◽  
Vol 264 (5166) ◽  
pp. 1753-1756 ◽  
Author(s):  
S Chiruvolu ◽  
S Walker ◽  
J Israelachvili ◽  
F. Schmitt ◽  
D Leckband ◽  
...  
Keyword(s):  
Self Assembly ◽  
Specific Binding ◽  
Higher Order ◽  
Site Specific

Protein Assembly Through Site-specific Interactions with Gold Nanoparticles

2006 ◽  
Vol 951 ◽  
Author(s):  
Minghui Hu ◽  
Luping Qian ◽  
Raymond P Briñas ◽  
Elena S Lymar ◽  
James F Hainfeld
Keyword(s):  
Gold Nanoparticles ◽  
Specific Binding ◽  
Nitrilotriacetic Acid ◽  
Protein Assembly ◽  
20S Proteasome ◽  
Universal Method ◽  
Specific Interactions ◽  
Adenovirus Serotype ◽  
Site Specific ◽  
Nanoparticle Assemblies

ABSTRACTA universal method is described to design and construct protein-nanoparticle assemblies controlled by nanoparticle functionality, and placement of genetic tag into proteins. Well-defined binding complexes of nanoparticles and two proteins, the adenovirus serotype 12 knob and the mycobacterium tuberculosis 20S proteasome, were formed through site-specific binding between 6x-histidine tags in proteins and nickel-nitrilotriacetic acid functional groups on gold nanoparticles.

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