Higher order self-assembly of vesicles by site-specific binding

Science ◽  
1994 ◽  
Vol 264 (5166) ◽  
pp. 1753-1756 ◽  
Author(s):  
S Chiruvolu ◽  
S Walker ◽  
J Israelachvili ◽  
F. Schmitt ◽  
D Leckband ◽  
...  
Keyword(s):  
Self Assembly ◽  
Specific Binding ◽  
Higher Order ◽  
Site Specific

scholarly journals DNA-mediated self-assembly of gold nanoparticles on protein superhelix

2018 ◽  
Author(s):  
Tao Zhang ◽  
Ingemar André
Keyword(s):  
Gold Nanoparticles ◽  
Self Assembly ◽  
De Novo ◽  
Specific Binding ◽  
Protein Structures ◽  
Spatial Arrangement ◽  
Inorganic Nanoparticles ◽  
Covalent Attachment ◽  
Site Specific ◽  
Conjugation Method

AbstractRecent advances in protein engineering have enabled methods to control the self-assembly of protein on various length-scales. One attractive application for designed proteins is to direct the spatial arrangement of nanomaterials of interest. Until now, however, a reliable conjugation method is missing to facilitate site-specific positioning. In particular, bare inorganic nanoparticles tend to aggregate in the presence of buffer conditions that are often required for the formation of stable proteins. Here, we demonstrated a DNA mediated conjugation method to link gold nanoparticles with protein structures. To achieve this, we constructed de novo designed protein fibers based on previously published uniform alpha-helical units. DNA modification rendered gold nanoparticles with increased stability against ionic solutions and the use of complementary strands hybridization guaranteed the site-specific binding to the protein. The combination of high resolution placement of anchor points in designed protein assemblies with the increased control of covalent attachment through DNA binding can enable investigations of multilevel physical coupling events of nanocomponents on protein templates and expand the application of protein structures to material sciences.

Reactive Nanopattern in a Triple Structured Bio-Inspired Honeycomb Film as a Clickable Platform

2018 ◽  
Author(s):  
Pierre Marcasuzaa ◽  
Samuel Pearson ◽  
Karell Bosson ◽  
Laurence Pessoni ◽  
Jean-Charles Dupin ◽  
...  
Keyword(s):  
Self Assembly ◽  
Double Porosity ◽  
Surface Groups ◽  
Specific Chemical ◽  
Site Specific ◽  
Surface Functionality ◽  
Wenzel State ◽  
Breath Figure ◽  
Responsive Surfaces ◽  
Secondary Population

A hierarchically structured platform was obtained from spontaneous self-assembly of a poly(styrene)-<i>b</i>-poly(vinylbenzylchloride) (PS-<i>b</i>-PVBC) block copolymer (BCP) during breath figure (BF) templating. The BF process using a water/ethanol atmosphere gave a unique double porosity in which hexagonally arranged micron-sized pores were encircled by a secondary population of smaller, nano-sized pores. A third level of structuration was simultaneously introduced between the pores by directed BCP self-assembly to form out-of-the-plane nano-cylinders, offering very rapid bottom-up access to a film with unprecedented triple structure which could be used as a reactive platform for introducing further surface functionality. The surface nano-domains of VBC were exploited as reactive nano-patterns for site-specific chemical functionalization by firstly substituting the exposed chlorine moiety with azide, then “clicking” an alkyne by copper (I) catalyzed azide-alkyne Huisgen cycloaddition (CuAAC). Successful chemical modification was verified by NMR spectroscopy, FTIR spectroscopy, and XPS, with retention of the micro- and nanostructuration confirmed by SEM and AFM respectively. Protonation of the cyclotriazole surface groups triggered a switch in macroscopic behavior from a Cassie-Baxter state to a Wenzel state, highlighting the possibility of producing responsive surfaces with hierarchical structure.

scholarly journals Polymerisation-induced self-assembly (PISA) as a straightforward formulation strategy for stimuli-responsive drug delivery systems and biomaterials: recent advances

2021 ◽  
Vol 9 (1) ◽  
pp. 38-50
Author(s):  
Hien Phan ◽  
Vincenzo Taresco ◽  
Jacques Penelle ◽  
Benoit Couturaud
Keyword(s):  
Drug Delivery ◽  
Block Copolymers ◽  
Drug Release ◽  
Self Assembly ◽  
Drug Delivery Systems ◽  
Amphiphilic Block Copolymers ◽  
Stimuli Responsive ◽  
Site Specific ◽  
On Demand ◽  
Redox Agents

Stimuli-responsive amphiphilic block copolymers obtained by PISA have emerged as promising nanocarriers for enhancing site-specific and on-demand drug release in response to a range of stimuli such as pH, redox agents, light or temperature.

scholarly journals Site-Specific Binding of Non-Site-Specific Ions

2019 ◽  
Vol 116 (12) ◽  
pp. 2237-2239 ◽  
Author(s):  
Shi-Jie Chen
Keyword(s):  
Specific Binding ◽  
Site Specific

scholarly journals Site-specific binding of wild-type p53 to cellular DNA is inhibited by SV40 T antigen and mutant p53.

1992 ◽  
Vol 6 (10) ◽  
pp. 1886-1898 ◽  
Author(s):  
J Bargonetti ◽  
I Reynisdottir ◽  
P N Friedman ◽  
C Prives
Keyword(s):  
Specific Binding ◽  
T Antigen ◽  
Mutant P53 ◽  
Wild Type ◽  
Sv40 T Antigen ◽  
Site Specific ◽  
Wild Type P53 ◽  
Cellular Dna

On-demand shape transformation of polymer vesicles via site-specific isomerization of hydrazone photoswitches in monodisperse hydrophobic oligomers

2021 ◽  
Author(s):  
Valene Wang ◽  
Jiwon Kim ◽  
Junyoung Kim ◽  
Seul Woo Lee ◽  
Kyoung Taek Kim
Keyword(s):  
Drug Delivery ◽  
Block Copolymers ◽  
Conformational Change ◽  
Self Assembly ◽  
Shape Control ◽  
Shape Transformation ◽  
Site Specific ◽  
On Demand ◽  
Polymer Vesicles

The shape control of nanostructures formed by the solution self-assembly of block copolymers is of significance for drug delivery. In particular, site-specific perturbation resulting in the conformational change of the...

A Protein Patterning Technique And Its Application In Bio-Inspired Self-Assembly

2002 ◽  
Vol 735 ◽  
Author(s):  
D. Guo ◽  
H. McNally ◽  
M. Pingle ◽  
D. Bergstrom ◽  
R. Bashir
Keyword(s):  
Self Assembly ◽  
Specific Binding ◽  
Gold Atom ◽  
Protein Patterning ◽  
Contact Mode ◽  
Microelectronic Fabrication ◽  
Micron Size ◽  
Patterning Technique ◽  
Lift Off ◽  
20 Nm

ABSTRACTProtein patterning techniques are crucial for the development of antibody-based biosensor and the study of controlled cell growth. This paper discusses a protein patterning technique based on microelectronic fabrication, DNA hybridization and biotin-streptavidin pair. A gold-on-silicon-dioxide substrate with micron size pattern was fabricated with photolithography and lift-off process. The average surface roughness of the gold pattern is 4.3 nm, measured by contact mode AFM. Thiol derivatized single stranded DNA was attached to the gold pattern surface by the chemical bonding between gold atom and sulfur atom. Surface attached DNA was then hybridized with a biotin conjugated complementary DNA sequence. Thus, the gold pattern was translated into a biotin pattern with similar resolution. Fluorescein conjugated streptavidin was patterned as demonstration. Fluorescence microscopy shows relative uniform streptavidin coverage of micron resolution and low background non-specific binding. The proposed protein patterning technique takes advantage of the high resolution of modern microelectronic fabrication. It has the potential of reaching sub-micron resolution. The biotin-streptavidin pair provides extremely specific and stable linking for protein immobilization. To show its application in biological inspired self-assembly, this technique was used successfully in the self-assembly of 20 nm streptavidin conjugated gold particles.

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