Effects of Cyromazine on Larval Survival, Pupation, and Adult Emergence of Colorado Potato Beetle (Coleoptera: Chrysomelidae)

1994 ◽  
Vol 87 (3) ◽  
pp. 577-582 ◽  
Author(s):  
J. M. Sirota ◽  
E. Grafius
Keyword(s):  
Colorado Potato Beetle ◽  
Adult Emergence ◽  
Larval Survival ◽  
Potato Beetle

Tomatillo Resistance to Colorado Potato Beetle (Coleoptera: Chrysomelidae)

2002 ◽  
Vol 37 (3) ◽  
pp. 244-253
Author(s):  
Lori J. Kroiss ◽  
Karen L. Broz ◽  
Cindy B. S. Tong ◽  
W. D. Hutchison
Keyword(s):  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Larval Survival ◽  
Negative Effects ◽  
Leaf Extracts ◽  
In Vivo Assays ◽  
Potato Beetle ◽  
Leptinotarsa Decemlineata Say ◽  
Trichome Exudates

Tomatillo (Physalis ixocarpa Brot ex Hornem) resistance to the Colorado potato beetle, Leptinotarsa decemlineata (Say), was evaluated in the laboratory. In vivo assays confirmed that L. decemlineata larvae fed tomatillo leaves had lower weights and survival than larvae fed potato leaves. Larvae fed potato leaves coated with α-tomatine also had lower weights and survival than controls. Larvae were also fed potato leaves coated with tomatillo leaf extracts. These larvae exhibited lower weights but equal survival compared with the controls. Removal of trichome exudates from tomatillo leaves did not affect the detrimental effects of the leaves on larvae. These results confirm the negative effects of tomatillo leaves and α-tomatine on L. decemlineata larval survival and weight. However, HPLC and mass spectrometry results show that α-tomatine was not present in the tomatillo leaf samples we studied. These results suggest that further research is warranted to isolate compounds for further plant resistance research within tomatillo.

scholarly journals Validating the potential of double-stranded RNA targeting Colorado potato beetle mesh gene in laboratory and field trials

2020 ◽  
Author(s):  
Marko Petek ◽  
Anna Coll ◽  
Rok Ferenc ◽  
Jaka Razinger ◽  
Kristina Gruden
Keyword(s):  
Colorado Potato Beetle ◽  
Field Trial ◽  
Larval Growth ◽  
Field Trials ◽  
Larval Survival ◽  
Innovative Technology ◽  
Feeding Experiments ◽  
Potato Beetle

ABSTRACTColorado potato beetle (CPB) is an agricultural pest of solanaceous crops, notorious for its rapid resistance development to chemical pesticides. Foliar spraying of dsRNA formulations is a promising innovative technology providing highly specific and environmentally acceptable option for CPB management.We designed dsRNA to silence CPB mesh gene (dsMESH) and performed laboratory feeding trials to assess impacts on beetle survival and development. We compared the effectiveness of in-vivo and in-vitro produced dsRNA in a series of laboratory experiments. We additionally performed a field trial in which the efficacy of dsRNA sprayed onto potato foliage was compared to a spinosad-based insecticide.We showed that dsMESH ingestion consistently and significantly impaired larval growth and decreased larval survival in laboratory feeding experiments. In-vivo produced dsRNA performed similarly as in-vitro synthesised dsRNA in laboratory settings. In the field trial, dsMESH was as effective in controlling CPB larvae as a commercial spinosad insecticide, its activity was however slower. We discuss limitations and benefits of a potential dsMESH-based CPB management strategy and list some important RNAi based CPB research topics, which will have to be addressed in future.

Citrus limonoid effects on Colorado potato beetle larval survival and development

1996 ◽  
Vol 80 (3) ◽  
pp. 503-510 ◽  
Author(s):  
Kathleen D. Murray ◽  
Eleanor Groden ◽  
Francis A. Drummond ◽  
A. Randall Alford ◽  
Richard H. Storch ◽  
...  
Keyword(s):  
Colorado Potato Beetle ◽  
Larval Survival ◽  
Potato Beetle ◽  
Survival And Development

scholarly journals Biological efficacy of some biorational and conventional insecticides in the control of different stages of the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)

2010 ◽  
Vol 46 (No. 3) ◽  
pp. 123-134 ◽  
Author(s):  
M.A.M. Osman
Keyword(s):  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Residual Activity ◽  
Adult Emergence ◽  
Mortality Rates ◽  
Field Conditions ◽  
Biological Efficacy ◽  
Potato Beetle ◽  
Leptinotarsa Decemlineata Say ◽  
Third Instar Larvae

The biological efficacy of some biorational and conventional insecticides against different stages of Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) was evaluated under laboratory and semi-field conditions. Seven different commercial products were tested, including the biorational insecticides: Spinosad, Mectin, Fitoverm, Match, Neemix in addition to two conventional insecticides: Actara and Actellic. Data indicated that all tested insecticides showed low toxic effects to L. decemlineata eggs, but most hatching neonates died shortly after hatching. All tested insecticides at their field rates showed high toxicity to larvae of L. decemlineata. The highest mortality was obtained in earlier instars, as compared to older ones, and mortality increased with the time of exposure. Moreover, the lower concentrations (up to 25% of the field rate) of Actara, Mectin, Spinosad, and Fitoverm showed high efficacy against L. decemlineata third instar larvae. Also, Actara caused the highest mortality in L. decemlineata adults, followed by Spinosad, Mectin, and Fitoverm as compared to Actellic, Match, and Neemix. In pupal bioassay, Fitoverm caused the greatest reduction in L. decemlineata adult emergence followed by Mectin, Actara, Actellic and Spinosad. In translocation bioassays, Actara caused the highest mortality in L. decemlineata 3<sup>rd</sup> instar larvae or adults followed by Spinosad and Mectin. The residual activity of tested insecticides against third instar larvae was also evaluated. Actara, Spinosad, and Mectin were more persistent under field conditions, consequently the mortality rates after 30 days of application were 46.67%, 44.44%, and 35.56%, respectively.

Effect of Amitraz on Colorado Potato Beetle (Coleoptera: Chrysomelidae) Feeding, Development and Survival

1996 ◽  
Vol 31 (1) ◽  
pp. 89-101 ◽  
Author(s):  
Craig A. Longtine ◽  
Geoff W. Zehnder ◽  
Edward B. Radcliffe
Keyword(s):  
Colorado Potato Beetle ◽  
Adult Emergence ◽  
Field Trials ◽  
Long Term Effects ◽  
Potato Beetle ◽  
Adult Feeding ◽  
Leptinotarsa Decemlineata Say ◽  
Contact Exposure ◽  
Early Instar ◽  
And Control

Effects of amitraz, a formamidine insecticide, were studied in Colorado potato beetle, Leptinotarsa decemlineata (Say), populations from Minnesota, North Dakota and Virginia. Contact exposure or ingestion of leaves dipped in 4000 ppm amitraz was not lethal to adults. However, adult feeding was reduced 50% upon exposure to 90 ppm amitraz, a rate &lt;1/8 that recommended by the manufacturer for testing as a foliar insecticide. Ingestion of foliage treated with 945 ppm amitraz by early instar larvae had long-term effects on Colorado potato beetle development and survival, delaying adult emergence 4.7 days and causing 87% mortality. In 72 h bioassays, LC50s by contact exposure or ingestion were &gt; 3.2× the suggested field rate. LC50s determined by exposure of larvae to treated foliage were greater than LC50s determined by immersing larvae. Egg hatch was not reduced by application of ≤ 1840 ppm amitraz. In field trials, amitraz reduced defoliation as effectively as esfenvalerate, the insecticide of choice when these trials were conducted. Amitraz-treated plots had yields intermediate between esfenvalerate and control treatments.

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