RNA-based biocontrol for Colorado potato beetle and corn rootworm control

2016 ◽  
Author(s):  
Pascale Feldmann
Keyword(s):  
Colorado Potato Beetle ◽  
Corn Rootworm ◽  
Potato Beetle

scholarly journals AFLP-Based Genetic Linkage Map of the Colorado Potato Beetle Leptinotarsa decemlineata: Sex Chromosomes and a Pyrethroid-Resistance Candidate Gene

Genetics ◽  
2001 ◽  
Vol 158 (2) ◽  
pp. 695-700 ◽  
Author(s):  
David J Hawthorne
Keyword(s):  
Candidate Gene ◽  
Linkage Map ◽  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Sex Chromosome ◽  
Pyrethroid Resistance ◽  
Codominant Markers ◽  
Potato Beetle

Abstract A genetic linkage map was constructed from an intraspecific cross of the Colorado potato beetle, Leptinotarsa decemlineata. This is an initial step toward mapping the loci that underlie important phenotypes associated with insect adaptation to an agroecosystem. The map was made with 172 AFLP and 10 anonymous codominant markers segregating among 74 backcross (BC1) individuals. Markers were mapped to 18 linkage groups and a subset of the markers with a mean intermarker distance of 11.1 cM is presented. A pyrethroid-resistance candidate gene, LdVssc1, was placed onto the map as well. The sex chromosome was identified by exploiting the XO nature of sex determination in this species using patterns of variation at LdVssc1 and the codominant markers.

scholarly journals Field Efficacy of Steinernema sp. (Rhabditida: Steinernematidae) on the Colorado Potato Beetle Overwintering Generation

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1464
Author(s):  
Maja Čačija ◽  
Renata Bažok ◽  
Majda Kolenc ◽  
Tena Bujas ◽  
Zrinka Drmić ◽  
...  
Keyword(s):  
Colorado Potato Beetle ◽  
First Generation ◽  
Control Measures ◽  
Recommended Dose ◽  
Steinernema Feltiae ◽  
First Year ◽  
Potato Beetle ◽  
Field Efficacy ◽  
Second Year ◽  
Economic Pest

Colorado potato beetle (CPB) is an economic pest of potato that has developed resistance to all classes of chemical insecticides, thus requiring alternative control measures. As a potential solution, entomopathogenic nematodes (EPNs) have proven effective in suppressing this pest, but their efficacy against overwintering generations of CPB in Croatia has not been sufficiently researched. The aim of this two-year (2018–2019) field study was to determine the efficacy of Steinernema feltiae and Steinernema carpocapsae applied to overwintering CPB adults. EPNs were applied at three doses (7.5 mil./10 m2, 5.0 mil./10 m2 (the recommended dose) and 2.5 mil./10 m2) by watering the soil where the adults were overwintering. The first-year results were satisfactory for both EPNs: the efficacy of S. feltiae ranged from 79.03% to 100.00%, while the efficacy of S. carpocapsae ranged from 77.32% to 96.22%. In the second year, the highest efficacy (69.57%) was obtained using the recommended dose of S. feltiae. Although the results are not consistent across the two years of our study and suggest further research, they indicate that EPNs have great potential in controlling overwintering CPB generations to reduce first generation abundance and damage, and also to prevent the spread of new generations to surrounding potato growing areas.

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