Genetic Variation in Larval Survival and Growth and Response to Selection by Colorado Potato Beetle (Coleoptera: Chrysomelidae) on Tomato

1997 ◽  
Vol 26 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Wenhua Lu ◽  
George G. Kennedy ◽  
Fred Gould
Keyword(s):  
Genetic Variation ◽  
Colorado Potato Beetle ◽  
Larval Survival ◽  
Response To Selection ◽  
Potato Beetle ◽  
Survival And Growth

Tomatillo Resistance to Colorado Potato Beetle (Coleoptera: Chrysomelidae)

2002 ◽  
Vol 37 (3) ◽  
pp. 244-253
Author(s):  
Lori J. Kroiss ◽  
Karen L. Broz ◽  
Cindy B. S. Tong ◽  
W. D. Hutchison
Keyword(s):  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Larval Survival ◽  
Negative Effects ◽  
Leaf Extracts ◽  
In Vivo Assays ◽  
Potato Beetle ◽  
Leptinotarsa Decemlineata Say ◽  
Trichome Exudates

Tomatillo (Physalis ixocarpa Brot ex Hornem) resistance to the Colorado potato beetle, Leptinotarsa decemlineata (Say), was evaluated in the laboratory. In vivo assays confirmed that L. decemlineata larvae fed tomatillo leaves had lower weights and survival than larvae fed potato leaves. Larvae fed potato leaves coated with α-tomatine also had lower weights and survival than controls. Larvae were also fed potato leaves coated with tomatillo leaf extracts. These larvae exhibited lower weights but equal survival compared with the controls. Removal of trichome exudates from tomatillo leaves did not affect the detrimental effects of the leaves on larvae. These results confirm the negative effects of tomatillo leaves and α-tomatine on L. decemlineata larval survival and weight. However, HPLC and mass spectrometry results show that α-tomatine was not present in the tomatillo leaf samples we studied. These results suggest that further research is warranted to isolate compounds for further plant resistance research within tomatillo.

scholarly journals Validating the potential of double-stranded RNA targeting Colorado potato beetle mesh gene in laboratory and field trials

2020 ◽  
Author(s):  
Marko Petek ◽  
Anna Coll ◽  
Rok Ferenc ◽  
Jaka Razinger ◽  
Kristina Gruden
Keyword(s):  
Colorado Potato Beetle ◽  
Field Trial ◽  
Larval Growth ◽  
Field Trials ◽  
Larval Survival ◽  
Innovative Technology ◽  
Feeding Experiments ◽  
Potato Beetle

ABSTRACTColorado potato beetle (CPB) is an agricultural pest of solanaceous crops, notorious for its rapid resistance development to chemical pesticides. Foliar spraying of dsRNA formulations is a promising innovative technology providing highly specific and environmentally acceptable option for CPB management.We designed dsRNA to silence CPB mesh gene (dsMESH) and performed laboratory feeding trials to assess impacts on beetle survival and development. We compared the effectiveness of in-vivo and in-vitro produced dsRNA in a series of laboratory experiments. We additionally performed a field trial in which the efficacy of dsRNA sprayed onto potato foliage was compared to a spinosad-based insecticide.We showed that dsMESH ingestion consistently and significantly impaired larval growth and decreased larval survival in laboratory feeding experiments. In-vivo produced dsRNA performed similarly as in-vitro synthesised dsRNA in laboratory settings. In the field trial, dsMESH was as effective in controlling CPB larvae as a commercial spinosad insecticide, its activity was however slower. We discuss limitations and benefits of a potential dsMESH-based CPB management strategy and list some important RNAi based CPB research topics, which will have to be addressed in future.

scholarly journals Elevated rates of positive selection drive the evolution of pestiferousness in the Colorado potato beetle (Leptinotarsa decemlineata, Say)

2019 ◽  
Author(s):  
Zachary P. Cohen ◽  
Kristian Brevik ◽  
Yolanda H. Chen ◽  
David J. Hawthorne ◽  
Benjamin D. Weibel ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Transposable Element ◽  
Positive Selection ◽  
Gene Family ◽  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Relative Importance ◽  
Rapid Adaptation ◽  
Potato Beetle ◽  
Element Variation

AbstractInsect pests are characterized by expansion, preference and performance on agricultural crops, high fecundity and rapid adaptation to control methods, which we collectively refer to as pestiferousness. Which organismal traits and evolutionary processes facilitate certain taxa becoming pests remains an outstanding question for evolutionary biologists. In order to understand these features, we set out to test the relative importance of genomic properties that underlie the rapid evolution of pestiferousness in the emerging pest model: the Colorado potato beetle (CPB), Leptinotarsa decemlineata Say. Within the Leptinotarsa genus, only CPB has risen to pest status on cultivated Solanum. Using whole genomes from ten closely related Leptinotarsa species, we reconstructed a high-quality species tree of this genus. Within this phylogenetic framework, we tested the relative importance of four drivers of rapid adaptation: standing genetic variation, gene family expansion and contraction, transposable element variation, and protein evolution. Throughout approximately 20 million years of divergence, Leptinotarsa show little evidence of gene family turnover or transposable element variation contributing to pest evolution. However, there is a clear pattern of pest lineages experiencing greater rates of positive selection on protein coding genes, as well as retaining higher levels of standing genetic variation. We also identify a suite of positively selected genes unique to the Colorado potato beetle that are directly associated with pestiferousness. These genes are involved in xenobiotic detoxification, chemosensation, and hormones linked with pest behavior and physiology.

scholarly journals Genome resequencing reveals rapid, repeated evolution in the Colorado potato beetle, Leptinotarsa decemlineata

2021 ◽  
Author(s):  
Benjamin Pélissié ◽  
Yolanda H. Chen ◽  
Zachary P. Cohen ◽  
Michael S. Crossley ◽  
David J. Hawthorne ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Insecticide Resistance ◽  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Management Practices ◽  
Rapid Evolution ◽  
Genome Resequencing ◽  
Novel Mutations ◽  
Evolutionary Mechanisms ◽  
Potato Beetle

AbstractBackgroundInsecticide resistance and rapid pest evolution threatens food security and the development of sustainable agricultural practices. An improved understanding of the evolutionary mechanisms that allow pests to rapidly adapt to novel control tactics will help prevent economically damaging outbreaks. The Colorado potato beetle (CPB), Leptinotarsa decemlineata, is a global super-pest that rapidly evolves resistance to insecticides. Using whole genome resequencing and transcriptomic data focused on its ancestral and pest range in North America, we assess evidence for three, non-mutually exclusive models of rapid evolution: pervasive selection on novel mutations, rapid regulatory evolution, and repeated selection on standing genetic variation.ResultsPopulation genomic analysis demonstrates that CPB is geographically structured, even among recently established pest populations. Pest populations exhibit only modest reductions in nucleotide diversity, relative to non-pest ancestral populations, and show evidence of recent demographic expansion. Genome scans of selection provide clear signatures of repeated adaptation across different CPB populations, with especially strong evidence that insecticide resistance involves selection of different genes in different populations. Similarly, analyses of gene expression show that constitutive upregulation of candidate insecticide resistance genes drives distinctive population patterns.ConclusionCPB evolves insecticide resistance repeatedly across agricultural regions, and oftentimes at the same loci, supporting a prominent role of polygenic evolution from standing genetic variation. Despite expectations, we do not find support for strong selection on novel mutations, or rapid evolution from selection on regulatory genes. An important future goal will be to understand how polygenic resistance phenotypes spread among local pest populations, in order to refine integrated pest management practices to maintain the efficacy and sustainability of novel control techniques.

Citrus limonoid effects on Colorado potato beetle larval survival and development

1996 ◽  
Vol 80 (3) ◽  
pp. 503-510 ◽  
Author(s):  
Kathleen D. Murray ◽  
Eleanor Groden ◽  
Francis A. Drummond ◽  
A. Randall Alford ◽  
Richard H. Storch ◽  
...  
Keyword(s):  
Colorado Potato Beetle ◽  
Larval Survival ◽  
Potato Beetle ◽  
Survival And Development
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