The influence of culture medium on the comparative in-vitro activity of amoxycillin and amoxycillin/clavulanic acid against Branhamella catarrhalis

1987 ◽  
Vol 20 (2) ◽  
pp. 285-286 ◽  
Author(s):  
F. SORIANO ◽  
M. JIMÉNEZ-ARRIERO ◽  
R. FERNÁNDEZ-ROBLAS
Keyword(s):  
Clavulanic Acid ◽  
Culture Medium ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Branhamella Catarrhalis

In vitro activity of ticarcillin plus clavulanic acid against bacteria isolated in three centers

1984 ◽  
Vol 3 (3) ◽  
pp. 203-206 ◽  
Author(s):  
A. L. Barry ◽  
L. W. Ayers ◽  
T. L. Gavan ◽  
E. H. Gerlach ◽  
R. N. Jones
Keyword(s):  
Clavulanic Acid ◽  
Vitro Activity ◽  
In Vitro Activity

scholarly journals In vitro Antischistosomal Activity of Clerodendrum umbellatum Poir (Labiateae) Leaves Aqueous Extract and Derived Fractions against Schistosoma mansoni Adult Worms

2020 ◽  
pp. 40-49
Author(s):  
Mérimé Christian Kenfack ◽  
Hermine Boukeng Jatsa ◽  
Nestor Gipwe Feussom ◽  
Emilienne Tienga Nkondo ◽  
Ulrich Membe Femoe ◽  
...  
Keyword(s):  
Motor Activity ◽  
Schistosoma Mansoni ◽  
Aqueous Extract ◽  
Culture Medium ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Phytochemical Screening ◽  
Methanol Fraction ◽  
Antischistosomal Activity

Aims: Treatment against schistosomiasis relies on praziquantel. Its treatment failure and the possible development of resistant schistosomes strains have been reported in the literature. Clerodendrum umbellatum leaves are used in Africa for the treatment of intestinal helminthiasis. The aim of this study was to evaluate the in vitro activity of C. umbellatum leaves aqueous extract and derived fractions on Schistosoma mansoni adult worms. Methodology: Five male and five female Schistosoma mansoni adult worms were incubated in each well for 48 h in a GMEM culture medium with C. umbellatum aqueous extract (125 to 4000 µg/mL) or its n-hexane, ethyl acetate and methanol fractions or the aqueous residue (62.5 to 2000 µg/mL). The main parameters assessed were the worm’s mortality and the reduction of motor activity. Phytochemical screening of all our tested substances was also performed. The cytotoxicity assay using mouse melanoma liver cells line was performed on the aqueous extract and on the most active fraction. Results: Our study shown that C. umbellatum leaves aqueous extract and its derived fractions promoted worm mortality. The aqueous extract disclosed a LC50 of 805.21 µg/mL while the LC50 of the methanol fraction was 343.10 µg/mL. With this lowest LC50, the methanol fraction from C. umbellatum aqueous extract was therefore the most active. Moreover, it showed low level of toxicity on hepatocytes. Incubation of worms with C. umbellatum aqueous extract and fractions also resulted in a significant reduction of the motor activity of survival worms with a 39.54 to 100% reduction after 48h. The phytochemical screening of C. umbellatum aqueous extract and fractions revealed the presence of alkaloids, phenols, flavonoids, tannins, saponins and terpenoids. Conclusion: The present study demonstrated the in vitro activity of C. umbellatum aqueous extract and derived fractions on S. mansoni adult worms and could then justify its empirical use to combat schistosomiasis.

Sign in / Sign up

Export Citation Format

Share Document