scholarly journals Molecular glass wool filtration as a new tool for sperm preparation

2007 ◽  
Vol 22 (5) ◽  
pp. 1405-1412 ◽  
Author(s):  
Sonja Grunewald ◽  
Werner Miska ◽  
Gisela Miska ◽  
Manja Rasch ◽  
Martin Reinhardt ◽  
...  
Keyword(s):  
Glass Wool ◽  
Sperm Preparation ◽  
Molecular Glass

Study of the ?-Mechanical Relaxation in Molecular Glass-Forming Liquids

1997 ◽  
Vol 7 (11) ◽  
pp. 1635-1650 ◽  
Author(s):  
A. Faivre ◽  
L. David ◽  
J. Perez
Keyword(s):  
Mechanical Relaxation ◽  
Glass Forming ◽  
Molecular Glass

EFFECTIVENESS OF "SWIM-UP" AND "GRADIENT" METHODS IN SPERM PREPARATION FOR ARTIFICIAL INSEMINATION

2014 ◽  
pp. 25-29
Author(s):  
Minh Tam Le ◽  
Van Trung Nguyen ◽  
Thi Tam An Nguyen ◽  
Quang Trung Phan ◽  
Ngoc Thanh Cao
Keyword(s):  
Gradient Method ◽  
Artificial Insemination ◽  
Sperm Count ◽  
Gradient Methods ◽  
Infertility Treatment ◽  
University Hospital ◽  
Preparation Methods ◽  
Sperm Preparation ◽  
Abnormal Morphology ◽  
Sperm Washing

Objectives: Artificial insemination with sperm preparation and inseminate to the uterus is a common method of infertility treatment. Currently two methods used for sperm washing is “swim-up” and “gradient”. Materials and methods:cross-sectional description in 166 cycles of artificial insemination in Hue University Hospital from April, 2012 to March, 2013 in order to compare effectiveness between “swim-up” and “gradient” methods. Samples were collected randomly into two methods. Results:Results of sperm preparation in both methods are equivalent in terms of the following parameters total sperm count, total number of progressive sperm, total good morphology sperm. However, “gradient” method results in higher number of progressive sperm in case with slow motility (38.3% vs. 26.1%) or abnormal morphology (34.9% vs. 19.7 %) compared with “swim-up” method. Pregnancy rates after artificial insemination were similar between two preparation methods. Conclusion:In case of slow motility sperm and abnormal morphology, “gradient” method should be used to increase the number of progressive sperms. Pregnancy outcome depends on many factors other than preparation methods. Key words: Artificial insemination, sperm preparation, infertility treatment

scholarly journals Replacement of Albumin by Preovulatory Oviductal Fluid in Swim-Up Sperm Preparation Method Modifies Boar Sperm Parameters and Improves In Vitro Penetration of Oocytes

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1202
Author(s):  
Sergio Navarro-Serna ◽  
Evelyne París-Oller ◽  
Ondrej Simonik ◽  
Raquel Romar ◽  
Joaquín Gadea
Keyword(s):  
Calcium Concentration ◽  
Preparation Method ◽  
Culture Media ◽  
Penetration Rate ◽  
Sperm Parameters ◽  
High Concentration ◽  
Sperm Preparation ◽  
Boar Sperm ◽  
Oviductal Fluid

More suitable and efficient methods to protect gametes from external harmful effects during in vitro handling can be achieved by adding preovulatory porcine oviductal fluid (pOF) to in vitro culture media. The objective of this study was to assess the swim-up procedure’s suitability as a sperm selection method using a medium supplemented with 1mg/mL BSA, 1% preovulatory pOF (v/v), 1% v/v pOF plus 1mg/mL BSA, and 5mg/mL BSA. After selection, various sperm parameters were studied, such as sperm recovery rate, sperm morphology, motility (by CASA), vitality, acrosome status and intracellular calcium (by flow cytometry) and ability to penetrate oocytes in vitro. Around 2% of sperm were recovered after swim-up, and the replacement of BSA by pOF showed a beneficial reduction of motility parameters calcium concentration, resulting in an increased penetration rate. The combination of albumin and oviductal fluid in the medium did not improve the sperm parameters results, whereas a high concentration of BSA increased sperm morphological abnormalities, motility, and acrosome damage, with a reduction of calcium concentration and penetration rate. In conclusion, the replacement of albumin by preovulatory oviductal fluid in the swim-up sperm preparation method modifies boar sperm parameters and improves the in vitro penetration of oocytes.

Fluorescent Molecular Glass Based on Hexadehydrotribenzo[12]annulene

2021 ◽  
Author(s):  
Yotaro Kasahara ◽  
Ichiro Hisaki ◽  
Tomoyuki Akutagawa ◽  
Takashi Takeda
Keyword(s):  
Glass Material ◽  
Molecular Glass

We prepared octylbenzoate-substituted [12]DBA (C8[12]DBA) as an organic molecular glass material. Even with a central large, planar π unit of [12]DBA, which is generally advantageous for the formation of a...

Sperm preparation techniques and their effect on semen quality and ICSI outcome

2007 ◽  
Vol 88 ◽  
pp. S149
Author(s):  
M.E. Hammadeh ◽  
L. EL-Masri ◽  
P. Rosenbaum ◽  
W. Schmidt ◽  
C. Fischer-Hammadeh
Keyword(s):  
Semen Quality ◽  
Sperm Preparation ◽  
Preparation Techniques
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