scholarly journals QUINACRINE FLUORESCENT CHROMOSOME ANALYSIS OF THE SNELL TRANSLOCATION IN THE MOUSE

Genetics ◽  
1972 ◽  
Vol 71 (4) ◽  
pp. 633-637
Author(s):  
D A Miller ◽  
P W Allderdice ◽  
R E Kouri ◽  
V G Dev ◽  
M S Grewal ◽  
...  
Keyword(s):  
Primary Cultures ◽  
Chromosome Analysis ◽  
Banding Patterns ◽  
Fluorescent Banding

ABSTRACT The chromosomes involved in the T(2;4)Sn (formerly designated T(5;8) Sn) or Snell translocation in the mouse have been identified as numbers 2 and 4 by analysis of the fluorescent banding patterns of quinacrine mustard-stained chromosomes in primary cultures from heterozygous and homozygous embryos.

Chromosome Analysis of Two Related Heteroploid Mouse Cell Lines by Quinacrine Fluorescence

1973 ◽  
Vol 12 (1) ◽  
pp. 263-274
Author(s):  
P. W. ALLDERDICE ◽  
O. J. MILLER ◽  
D. A. MILLER ◽  
D. WARBURTON ◽  
P. L. PEARSON ◽  
...  
Keyword(s):  
Cell Lines ◽  
Chromosome Analysis ◽  
Mouse Cell ◽  
Previous Method ◽  
Structural Rearrangements ◽  
Metaphase Chromosomes ◽  
Detailed Characterization ◽  
Banding Patterns ◽  
Fluorescent Banding ◽  
Quinacrine Fluorescence

The fluorescent banding patterns of quinacrine-stained metaphase chromosomes have been studied in 2 related mouse cell lines, A9 and a malignant derivative of A9, A9HT. In both cell lines virtually every chromosome has a distinctive banding pattern which permits its recognition. More than three quarters of the chromosomes have structural rearrangements, but the origin of nearly two thirds of the chromosomes could be determined by their banding patterns. The quinacrine fluorescence technique permits far more detailed characterization and comparison of heteroploid cell lines than any previous method. A9 and A9HT are karyologically quite similar, with many of the same marker chromosomes. There are, however, characteristic differences. A9HT, although it has a smaller average number of chromosomes per cell, appears to be more heterogeneous.

Fluorescent banding patterns of the chromosomes of the genus Salmo

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 193-197 ◽  
Author(s):  
Ruth B. Phillips ◽  
Sheila E. Hartley
Keyword(s):  
Great Britain ◽  
North America ◽  
Acrocentric Chromosome ◽  
Organizer Region ◽  
Nucleolar Organizer Region ◽  
Nucleolar Organizer ◽  
Salmo Gairdneri ◽  
Chromomycin A3 ◽  
Banding Patterns ◽  
Fluorescent Banding

The fluorescent banding patterns of the chromosomes of Salmo gairdneri (rainbow trout), Salmo trutta (brown trout), and Salmo salar (Atlantic salmon) from North America and Great Britain are described. Quinacrine stained a subset of C bands in S. gairdneri and S. salar from North America. No bright quinacrine (Q) bands were found on the chromosomes of S. salar from Great Britain or the chromosomes from any of the three stocks of S. trutta that were examined. Q bands were found at the centromeres of three to seven different chromosome pairs in S. gairdneri, including the pair that has been identified as the sex chromosome pair in some populations. In S. salar from North America the Q bands were found at the teleomeres of three to four chromosome pairs and at interstitial locations in the 10–13 large acrocentric chromosome pairs. Chromomycin A3 stained either the nucleolar organizer region or the adjacent heterochromatin or both in all three species. In S. trutta the entire short arm of the acrocentric chromosome containing the nucleolar organizer region always stained with chromomycin A3 while in S. gairdneri and S. salar the staining properties of the NOR and adjacent heterochromatin were polymorphic.Key words: banding, C-, Q-; Salmo; trout.

Characterization of the canine karyotype by counterstain-enhanced chromosome banding

1983 ◽  
Vol 25 (6) ◽  
pp. 616-621 ◽  
Author(s):  
B. Mayr ◽  
D. Schweizer ◽  
W. Schleger
Keyword(s):  
Actinomycin D ◽  
Mitotic Division ◽  
Banding Technique ◽  
Chromosome Identification ◽  
Banding Patterns ◽  
Distamycin A ◽  
Fluorescent Banding ◽  
Meiotic Chromosomes ◽  
Triple Staining

The application of the counterstain-contrasted fluorescent banding technique to canine chromosomes provided an improved capability to highlight specific heterochromatic regions and to produce well defined banding patterns both on mitotic and meiotic chromosomes. Triple staining with chromomycin A3 – distamycin A – DAPI revealed the occurrence of DA – DAPI positive heterochromatin in chromosomes 33, 36, 37, and 38. Pachytene nuclei present more favourable material for the detection of very small amounts of DA – DAPI material than mitotic division stages. Counterstain-enhanced chromomycin R-banding greatly facilitated chromosome identification. A standard R-band karyotype of Canis familiaris is proposed and described in some detail. DAPI – actinomycin D staining produced a QFH-type banding pattern and enhanced differentiation of some polymorphic regions.

scholarly journals Chromosome banding in the genus Pinus IV. Fluorescent banding patterns of chromosomes in eight taxa of haploxylone pines

2016 ◽  
Vol 11 (3) ◽  
pp. 61-71 ◽  
Author(s):  
Masahiro Hizume ◽  
Kozue N. Ohtaka ◽  
Kaoru M. Takeda ◽  
Satomi Fujii ◽  
Yoko Yamasaki ◽  
...  
Keyword(s):  
Chromosome Banding ◽  
Banding Patterns ◽  
Fluorescent Banding

Polytene chromosome maps of the melon fly Bactrocera cucurbitae (Diptera: Tephritidae)

Genome ◽  
2002 ◽  
Vol 45 (6) ◽  
pp. 1167-1174 ◽  
Author(s):  
Reza M Shahjahan ◽  
Farzana Yesmin
Keyword(s):  
Salivary Gland ◽  
Polytene Chromosome ◽  
Sex Chromosome ◽  
Polytene Chromosomes ◽  
Chromosome Analysis ◽  
Bactrocera Cucurbitae ◽  
Mitotic Chromosomes ◽  
Larval Brain ◽  
Banding Patterns ◽  
Melon Fly

Standard photographic maps of the polytene chromosomes are presented for the melon fly Bactrocera cucurbitae, a serious pest of fleshy fruits and vegetables. Five larval salivary gland polytene chromosomes (10 polytene arms) were isolated, and their characteristic features and landmarks have been recognized. Banding patterns of each of the polytene arms are presented, where variation in band intensity and puffs appear to reflect fundamental differences in chromosomes. The whole polytene genome has been typically mapped by dividing it into 100 sections and the subsections were lettered. The mitotic chromosomes of larval brain ganglia are also examined, five pairs of autosomes and an XX/XY sex chromosome pair. In addition, a heterochromatic mass corresponding to the sex chromosomes are observed in the polytene nuclei of salivary gland tissue. This investigation showed that B. cucurbitae has excellent cytological material for polytene chromosome analysis and proved to be very useful for obtaining more detailed genetic information on the pest's natural populations.Key words: Bactrocera cucurbitae, salivary gland, banding patterns, polytene maps.

Constitutional Chromoanagenesis of Distal 13q in a Young Adult with Recurrent Strokes

2016 ◽  
Vol 150 (1) ◽  
pp. 46-51 ◽  
Author(s):  
Rachel D. Burnside ◽  
April Harris ◽  
Darrow Speyer ◽  
W. Scott Burgin ◽  
David Z. Rose ◽  
...  
Keyword(s):  
Young Adult ◽  
Microarray Analysis ◽  
Chromosome Analysis ◽  
Speech Delay ◽  
Banding Patterns ◽  
Snp Microarray ◽  
Clinical Cytogenetics ◽  
Cytogenetic Testing ◽  
Recurrent Strokes ◽  
Complex Chromosome

Constitutional chromoanagenesis events, which include chromoanasynthesis and chromothripsis and result in highly complex rearrangements, have been reported for only a few individuals. While rare, these phenomena have likely been underestimated in a constitutional setting as technologies that can accurately detect such complexity are relatively new to the mature field of clinical cytogenetics. G-banding is not likely to accurately identify chromoanasynthesis or chromothripsis, since the banding patterns of chromosomes are likely to be misidentified or oversimplified due to a much lower resolution. We describe a patient who was initially referred for cytogenetic testing as a child for speech delay. As a young adult, he was referred again for recurrent strokes. Chromosome analysis was performed, and the rearrangement resembled a simple duplication of 13q32q34. However, SNP microarray analysis showed a complex pattern of copy number gains and a loss consistent with chromoanasynthesis involving distal 13q (13q32.1q34). This report emphasizes the value of performing microarray analysis for individuals with abnormal or complex chromosome rearrangements.

scholarly journals Chromosome banding in the genus Pinus V. Fluorescent banding patterns in 16 diploxylon pines

2016 ◽  
Vol 11 (4) ◽  
pp. 77-92
Author(s):  
Masahiro Hizume ◽  
Motonobu Arai ◽  
Yoko Yamasaki ◽  
Satomi Fujii ◽  
Kaoru M. Takeda ◽  
...  
Keyword(s):  
Chromosome Banding ◽  
Banding Patterns ◽  
Fluorescent Banding
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