Relationship of Centromeric Heterochromatin to Fluorescent Banding Patterns of Metaphase Chromosomes in the Mouse

Nature ◽  
1971 ◽  
Vol 231 (5304) ◽  
pp. 503-506 ◽  
Author(s):  
J. D. ROWLEY ◽  
W. F. BODMER
Keyword(s):  
Centromeric Heterochromatin ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Fluorescent Banding ◽  
Relationship Of

Chromosome Analysis of Two Related Heteroploid Mouse Cell Lines by Quinacrine Fluorescence

1973 ◽  
Vol 12 (1) ◽  
pp. 263-274
Author(s):  
P. W. ALLDERDICE ◽  
O. J. MILLER ◽  
D. A. MILLER ◽  
D. WARBURTON ◽  
P. L. PEARSON ◽  
...  
Keyword(s):  
Cell Lines ◽  
Chromosome Analysis ◽  
Mouse Cell ◽  
Previous Method ◽  
Structural Rearrangements ◽  
Metaphase Chromosomes ◽  
Detailed Characterization ◽  
Banding Patterns ◽  
Fluorescent Banding ◽  
Quinacrine Fluorescence

The fluorescent banding patterns of quinacrine-stained metaphase chromosomes have been studied in 2 related mouse cell lines, A9 and a malignant derivative of A9, A9HT. In both cell lines virtually every chromosome has a distinctive banding pattern which permits its recognition. More than three quarters of the chromosomes have structural rearrangements, but the origin of nearly two thirds of the chromosomes could be determined by their banding patterns. The quinacrine fluorescence technique permits far more detailed characterization and comparison of heteroploid cell lines than any previous method. A9 and A9HT are karyologically quite similar, with many of the same marker chromosomes. There are, however, characteristic differences. A9HT, although it has a smaller average number of chromosomes per cell, appears to be more heterogeneous.

A SUGGESTION FOR THE NOMENCLATURE OF THE FLUORESCENT BANDING PATTERNS IN HUMAN METAPHASE CHROMOSOMES

1971 ◽  
Vol 13 (2) ◽  
pp. 361-363 ◽  
Author(s):  
C. C. Lin ◽  
Irene A. Uchida ◽  
Elizabeth Byrnes
Keyword(s):  
Human Chromosomes ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Fluorescent Banding ◽  
Visual Identification ◽  
Fluorescent Technique ◽  
Characteristic Banding

With the application of fluorescent technique, it is now possible to recognize characteristic banding patterns in human chromosomes. A simple nomenclature for the bands is suggested according to their visual identification.

Advances in imaging SIMS studies of stained and BrdU-labelled human metaphase chromosomes

1995 ◽  
Vol 53 ◽  
pp. 932-933
Author(s):  
R. Levi-Setti ◽  
J. M. Chabala ◽  
R. Espinosa ◽  
M. M. Le Beau
Keyword(s):  
High Performance ◽  
Secondary Ion Mass Spectrometry ◽  
Analytical Sensitivity ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Sector Mass Spectrometer ◽  
Staining Methods ◽  
The University ◽  
Secondary Ion ◽  
Better Than

We have shown previously that isotope-labelled nucleotides in human metaphase chromosomes can be detected and mapped by imaging secondary ion mass spectrometry (SIMS), using the University of Chicago high resolution scanning ion microprobe (UC SIM). These early studies, conducted with BrdU- and 14C-thymidine-labelled chromosomes via detection of the Br and 28CN- (14C14N-> labelcarrying signals, provided some evidence for the condensation of the label into banding patterns along the chromatids (SIMS bands) reminiscent of the well known Q- and G-bands obtained by conventional staining methods for optical microscopy. The potential of this technique has been greatly enhanced by the recent upgrade of the UC SIM, now coupled to a high performance magnetic sector mass spectrometer in lieu of the previous RF quadrupole mass filter. The high transmission of the new spectrometer improves the SIMS analytical sensitivity of the microprobe better than a hundredfold, overcoming most of the previous imaging limitations resulting from low count statistics.

Mitosis and Interphase of the Highly Polyploid Palm Voanioalagerardii (2n = 606 ± 3)

2015 ◽  
Vol 147 (1) ◽  
pp. 70-79 ◽  
Author(s):  
Martin Röser
Keyword(s):  
Nuclear Gene ◽  
Point Of View ◽  
Fluorochrome Banding ◽  
Dna Amount ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
High Polyploid ◽  
Molecular Phylogenetic ◽  
Gene Data ◽  
First Time

The endemic, highly polyploid, monotypic Madagascan palm genus Voanioala (2n ≈ 606) was studied with regard to mitotic stages and interphase. Features of the cell cycle, morphology and sizes of metaphase chromosomes, fluorochrome banding patterns, and silver staining of NORs of such an extremely high polyploid organism are reported for the first time. On a whole, karyokinesis appears to be stable and efficient. A comparison with closely related palm taxa reveals that V. gerardii is 38-ploid, and comparison with the closely related genera Butia, Cocos (coconut) and Jubaea shows that Voanioala has lost ∼35% of its DNA amount subsequent to polyploidization and has suppressed between 74 and 88% of the original nucleolar organizers. About 10 active NORs are present in the nuclei. An auto- or allopolyploid origin of Voanioala is discussed with respect to currently available nuclear gene data. The biogeographic relations to Jubaeopsis, a closely related, monotypic, apparently likewise relict palm genus from eastern mainland South Africa are discussed. From a cytogenetic point of view, a common polyploid ancestor of both genera is most likely, but the available molecular phylogenetic data are not univocal.

Fluorescent banding patterns of the chromosomes of the genus Salmo

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 193-197 ◽  
Author(s):  
Ruth B. Phillips ◽  
Sheila E. Hartley
Keyword(s):  
Great Britain ◽  
North America ◽  
Acrocentric Chromosome ◽  
Organizer Region ◽  
Nucleolar Organizer Region ◽  
Nucleolar Organizer ◽  
Salmo Gairdneri ◽  
Chromomycin A3 ◽  
Banding Patterns ◽  
Fluorescent Banding

The fluorescent banding patterns of the chromosomes of Salmo gairdneri (rainbow trout), Salmo trutta (brown trout), and Salmo salar (Atlantic salmon) from North America and Great Britain are described. Quinacrine stained a subset of C bands in S. gairdneri and S. salar from North America. No bright quinacrine (Q) bands were found on the chromosomes of S. salar from Great Britain or the chromosomes from any of the three stocks of S. trutta that were examined. Q bands were found at the centromeres of three to seven different chromosome pairs in S. gairdneri, including the pair that has been identified as the sex chromosome pair in some populations. In S. salar from North America the Q bands were found at the teleomeres of three to four chromosome pairs and at interstitial locations in the 10–13 large acrocentric chromosome pairs. Chromomycin A3 stained either the nucleolar organizer region or the adjacent heterochromatin or both in all three species. In S. trutta the entire short arm of the acrocentric chromosome containing the nucleolar organizer region always stained with chromomycin A3 while in S. gairdneri and S. salar the staining properties of the NOR and adjacent heterochromatin were polymorphic.Key words: banding, C-, Q-; Salmo; trout.

scholarly journals Purification of the chromosomes of the Indian muntjac by flow sorting.

1976 ◽  
Vol 24 (1) ◽  
pp. 348-354 ◽  
Author(s):  
A V Carrano ◽  
J W Gray ◽  
D H Moore ◽  
J L Minkler ◽  
B H Mayall ◽  
...  
Keyword(s):  
Large Fraction ◽  
Centromere Region ◽  
Chromosomal Dna ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Indian Muntjac ◽  
Flow Sorting ◽  
Flow Microfluorometry ◽  
Mechanical Shearing ◽  
High Degree

Metaphase chromosomes were isolated from a male Indian muntjac cell line, were stained with ethidium bromide and were analyzed by flow microfluorometry to establish a deoxyribonucleic acid (DNA)-based karyotype. Five major peaks were evident on the chromosomal DNA distribution corresponding to the five chromosome types in this species. The amount of DNA in each chromosome was confirmed by cytophotometric measurements of intact metaphase spreads. The five chromosome types were separated by flow sorting at rates up to several hundred chromosomes per second. The sorted chromosomes were identified by morphology and by Giemsa banding patterns. The automsomes, Numbers 1, 2 and 3, and the X + 3 composite chromosome were separated with a high degree of purity (90%). The centromere region of the X + 3 chromosome was fragile to mechanical shearing, and during isolation a small proportion of these chromosomes broke into four segiments: the long arm, the short arm, the short arm plus centromere and the centromere region. A large fraction of the constitutive heterochromatin of this species is present in the centromere region of the X + 3 chromosome and in the Y chromosome; these two regions possess similar amounts of DNA and therefore sort together. Chromosome flow sorting is rapid, reproducible and precise; it allows the collection of microgram quantities of purified chromosomes.

scholarly journals A New Fluorescence Reaction in DNA Cytochemistry: Microscopic and Spectroscopic Studies on the Aromatic Diamidino Compound M&B 938

1997 ◽  
Vol 45 (1) ◽  
pp. 97-105 ◽  
Author(s):  
Juan C. Stockert ◽  
Clara I. Trigoso ◽  
Teresa Cuéllar ◽  
José L. Bella ◽  
José A. Lisanti
Keyword(s):  
Specific Binding ◽  
Linear Regression Analysis ◽  
Spectroscopic Studies ◽  
Centromeric Heterochromatin ◽  
Dna Ladder ◽  
Metaphase Chromosomes ◽  
Distamycin A ◽  
Ehrlich Ascites ◽  
Fluorescence Reaction ◽  
High Fluorescence

We describe the fluorescence properties and cytochemical applications of the aromatic diamidine M&B 938. Treatment of cell smears (chicken blood, Ehrlich ascites tumor, rat bone marrow, mouse mast cells, and Trypanosoma cruzi epimastigotes) with aqueous solutions of M&B 938 (0.5–1 μg/ml at pH 6–7; UV excitation) induced bright bluish-white fluorescence in DNA-containing structures (interphase and mitotic chromatin, AT-rich kinetoplast DNA of T. cruzi), which was abolished by previous DNA extraction. DNA was the unique fluorescent polyanion after staining with M&B 938 at neutral or alkaline pH, other polyanions such as RNA and heparin showing no emission. M&B 938-stained mouse metaphase chromosomes revealed high fluorescence of the AT-rich centromeric heterochromatin, and strong emission of heterochromatin in human chromosomes 1, 9, 15, 16, and Y was found after distamycin A counterstaining. On agarose gel electrophoresis, M&B 938-stained DNA markers appeared as fluorescent bands. The 1.635-kBP fragment from DNA ladder revealed a higher emission value than that expected from linear regression analysis. Spectroscopic studies showed bathochromic and hyperchromic shifts in the absorption spectrum of M&B 938 complexed with DNA, as well as strong enhancement of fluorescence at 420 nm. In the presence of poly(dA)-poly(dT), the emission of M&B 938 was 4.25-fold higher than with DNA; no fluorescence was observed with poly(dG)-poly(dC). Experimental results and considerations of the chemical structure suggest that the minor groove of AT regions of DNA could be the specific binding site for M&B 938, which shows interesting properties and useful applications as a new DNA fluorochrome.

Cytogenetic studies on Sauria (Reptilia). I. Mitotic chromosomes of the Agamidae

1988 ◽  
Vol 9 (3) ◽  
pp. 301-310 ◽  
Author(s):  
E. Solleder ◽  
M. Schmid
Keyword(s):  
Chromosome Number ◽  
Chromosome Morphology ◽  
Mitotic Chromosomes ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
The Family ◽  
Telocentric Chromosomes ◽  
Cytogenetic Studies ◽  
Pericentric Inversions ◽  
Dna Organization

The karotypes of nine species of the family Agamidae were analyzed with various banding techniques and conventional cytogenetic stainings. Whereas the examined species of the genera Calotes and Leiolepis exhibit conservative karyotypes, the chromosome number and chromosome morphology varies considerably within the genus Agama. This is attributed to centric fusions between telocentric chromosomes and pericentric inversions within the chromosomes. None of the species demonstrated multiple quinacrine banding patterns in the euchromatic segments of the metaphase chromosomes. This is probably due to the special DNA organization in these organisms.

scholarly journals Genetic Relationships among 10 Prunus Rootstock Species from China, Based on Simple Sequence Repeat Markers

2016 ◽  
Vol 141 (5) ◽  
pp. 520-526 ◽  
Author(s):  
Qijing Zhang ◽  
Dajun Gu
Keyword(s):  
Phylogenetic Analysis ◽  
Simple Sequence Repeat ◽  
Genetic Relationships ◽  
Sequence Repeat ◽  
Breeding Programs ◽  
Banding Patterns ◽  
Simple Sequence Repeat Markers ◽  
Ssr Primers ◽  
Relationship Of ◽  
Simple Sequence

To improve the efficiency of breeding programs for Prunus rootstock hybrids in China, we analyzed the subgenus status and relationship of 10 Chinese rootstock species, by using 24 sets of simple sequence repeat (SSR) primers. The SSR banding patterns and phylogenetic analysis indicated that subgenus Cerasus is more closely related to subgenus Prunophora than to subgenus Amygdalus, and that subgenus Lithocerasus is more closely related to subgenus Prunophora and subgenus Amygdalus than to subgenus Cerasus. In addition, Prunus triloba was more closely related to Prunus tomentosa than to the members of subgenus Amygdalus. Therefore, we suggest that P. tomentosa and P. triloba should be assigned to the same group, either to subgenus Lithocerasus or Prunophora, and we also propose potential parent combinations for future Prunus rootstock breeding.

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