Biguanides Metformin and Phenformin Generate Therapeutic Effects via AMP-Activated Protein Kinase/Extracellular-Regulated Kinase Pathways in an In Vitro Model of Graves' Orbitopathy

Thyroid ◽  
2018 ◽  
Vol 28 (4) ◽  
pp. 528-536 ◽  
Author(s):  
Ye Eon Han ◽  
Sena Hwang ◽  
Jin Hee Kim ◽  
Jung Woo Byun ◽  
Jin Sook Yoon ◽  
...  
Keyword(s):  
Protein Kinase ◽  
In Vitro Model ◽  
Therapeutic Effects ◽  
Graves Orbitopathy ◽  
Vitro Model ◽  
Extracellular Regulated Kinase ◽  
Amp Activated Protein Kinase

Effect of Tanshinone IIA in an In Vitro Model of Graves' Orbitopathy

2014 ◽  
Vol 55 (9) ◽  
pp. 5900 ◽  
Author(s):  
Soolienah Rhiu ◽  
Min Kyung Chae ◽  
Eun Jig Lee ◽  
Jong Bok Lee ◽  
Jin Sook Yoon
Keyword(s):  
In Vitro Model ◽  
Tanshinone Iia ◽  
Graves Orbitopathy ◽  
Vitro Model

scholarly journals Therapeutic Effect of Protocatechuic Aldehyde in an In Vitro Model of Graves' Orbitopathy

2016 ◽  
Vol 57 (10) ◽  
pp. 4055 ◽  
Author(s):  
Jung Woo Byun ◽  
Sena Hwang ◽  
Chan Woo Kang ◽  
Jin Hee Kim ◽  
Min Kyung Chae ◽  
...  
Keyword(s):  
Therapeutic Effect ◽  
In Vitro Model ◽  
Protocatechuic Aldehyde ◽  
Graves Orbitopathy ◽  
Vitro Model

scholarly journals Role of miR-146a in the Regulation of Inflammation in an In Vitro Model of Graves' Orbitopathy

2016 ◽  
Vol 57 (10) ◽  
pp. 4027 ◽  
Author(s):  
Sun Young Jang ◽  
Min Kyung Chae ◽  
Joon H. Lee ◽  
Eun Jig Lee ◽  
Jin Sook Yoon
Keyword(s):  
In Vitro Model ◽  
Graves Orbitopathy ◽  
Vitro Model

scholarly journals Protein tyrosine phosphatase 1B as a therapeutic target for Graves’ orbitopathy in an in vitro model

PLoS ONE ◽  
2020 ◽  
Vol 15 (8) ◽  
pp. e0237015 ◽  
Author(s):  
Hyeong Ju Byeon ◽  
Ji-Young Kim ◽  
JaeSang Ko ◽  
Eun Jig Lee ◽  
Kikkawa Don ◽  
...  
Keyword(s):  
Protein Tyrosine Phosphatase ◽  
Therapeutic Target ◽  
In Vitro Model ◽  
Tyrosine Phosphatase ◽  
Protein Tyrosine Phosphatase 1B ◽  
Protein Tyrosine ◽  
Graves Orbitopathy ◽  
Vitro Model

scholarly journals Mycoplasma fermentansand TNF-β interact to amplify immune-modulating cytokines in human lung fibroblasts

2006 ◽  
Vol 291 (4) ◽  
pp. L781-L793 ◽  
Author(s):  
James P. Fabisiak ◽  
Fei Gao ◽  
Robyn G. Thomson ◽  
Robert M. Strieter ◽  
Simon C. Watkins ◽  
...  
Keyword(s):  
Protein Kinase ◽  
In Vitro Model ◽  
Human Lung ◽  
Lung Fibroblasts ◽  
Mycoplasma Fermentans ◽  
Human Lung Fibroblasts ◽  
Toll Like Receptor 2 ◽  
Vitro Model ◽  
Specific Ligand

Mycoplasma can establish latent infections and are associated with arthritis, leukemia, and chronic lung disease. We developed an experimental model in which lung cells are deliberately infected with Mycoplasma fermentans. Human lung fibroblasts (HLF) were exposed to live M. fermentans and immune-modulating cytokine release was assessed with and without known inducers of cytokine production. M. fermentans increased IL-6, IL-8/CXCL8, MCP-1/CCL2, and Gro-α/CXCL1 production. M. fermentans interacted with TNF-β to release more IL-6, CXCL8, and CXCL1 than predicted by the responses to either stimulus alone. The effects of live infection were recapitulated by exposure to M. fermentans-derived macrophage-activating lipopeptide-2 (MALP-2), a Toll-like receptor-2- and receptor-6-specific ligand. The synergistic effect of combined stimuli was more pronounced with prolonged incubations. Preexposure to TNF-β sensitized the cells to subsequent MALP-2 challenge, but preexposure to MALP-2 did not alter the IL-6 response to TNF-β. Exposure to M. fermentans or MALP-2 did not enhance nuclear localization, DNA binding, or transcriptional activity of NF-κB and did not modulate early NF-κB activation in response to TNF-β. Application of specific inhibitors of various MAPKs suggested that p38 and JNK/stress-activated protein kinase were involved in early IL-6 release after exposure to TNF-β and M. fermentans, respectively. The combined response to M. fermentans and TNF-β, however, was uniquely sensitive to delayed application of SP-600125, suggesting that JNK/stress-activated protein kinase contributes to the amplification of IL-6 release. Thus M. fermentans interacts with stimuli such as TNF-β to amplify lung cell production of immune-modulating cytokines. The mechanisms accounting for this interaction can now be dissected with the use of this in vitro model.

Sign in / Sign up

Export Citation Format

Share Document