scholarly journals Metabolically Active Three-Dimensional Brown Adipose Tissue Engineered from White Adipose-Derived Stem Cells

2017 ◽  
Vol 23 (7-8) ◽  
pp. 253-262 ◽  
Author(s):  
Jessica P. Yang ◽  
Amy E. Anderson ◽  
Annemarie McCartney ◽  
Xavier Ory ◽  
Garret Ma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells ◽  
Brown Adipose

scholarly journals Effects of stem cells from inducible brown adipose tissue on diet-induced obesity in mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Enrique Calvo ◽  
Noelia Keiran ◽  
Catalina Núñez-Roa ◽  
Elsa Maymó-Masip ◽  
Miriam Ejarque ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Therapeutic Option ◽  
Metabolic Activation ◽  
Diet Induced Obesity ◽  
Brown Adipose ◽  
Obesity In Mice ◽  
Treatment Of Obesity ◽  
Visceral Adipose

AbstractAdipose-derived mesenchymal stem cells (ASCs) are a promising option for the treatment of obesity and its metabolic co-morbidities. Despite the recent identification of brown adipose tissue (BAT) as a potential target in the management of obesity, the use of ASCs isolated from BAT as a therapy for patients with obesity has not yet been explored. Metabolic activation of BAT has been shown to have not only thermogenic effects, but it also triggers the secretion of factors that confer protection against obesity. Herein, we isolated and characterized ASCs from the visceral adipose tissue surrounding a pheochromocytoma (IB-hASCs), a model of inducible BAT in humans. We then compared the anti-obesity properties of IB-hASCs and human ASCs isolated from visceral white adipose tissue (W-hASCs) in a murine model of diet-induced obesity. We found that both ASC therapies mitigated the metabolic abnormalities of obesity to a similar extent, including reducing weight gain and improving glucose tolerance. However, infusion of IB-hASCs was superior to W-hASCs in suppressing lipogenic and inflammatory markers, as well as preserving insulin secretion. Our findings provide evidence for the metabolic benefits of visceral ASC infusion and support further studies on IB-hASCs as a therapeutic option for obesity-related comorbidities.

Cardiac Stem Cells in Brown Adipose Tissue Express CD133 and Induce Bone Marrow Nonhematopoietic Cells to Differentiate into Cardiomyocytes

Stem Cells ◽  
2007 ◽  
Vol 25 (5) ◽  
pp. 1326-1333 ◽  
Author(s):  
Yoshihiro Yamada ◽  
Shin-ichiro Yokoyama ◽  
Xiang-Di Wang ◽  
Noboru Fukuda ◽  
Nobuyuki Takakura
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Cardiac Stem Cells ◽  
Brown Adipose

Transient overexpression of Pparγ2 and C/ebpα in mesenchymal stem cells induces brown adipose tissue formation

2013 ◽  
Vol 8 (3) ◽  
pp. 295-308 ◽  
Author(s):  
Dmitriy Sheyn ◽  
Gadi Pelled ◽  
Wafa Tawackoli ◽  
Susan Su ◽  
Shiran Ben-David ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Tissue Formation ◽  
Brown Adipose ◽  
Transient Overexpression

Human metabolically active brown adipose tissue derived stem cells

Cytotherapy ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. S69
Author(s):  
F. Silva ◽  
D. Holt ◽  
V. Vargas ◽  
D. Bull ◽  
A.N. Patel
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Brown Adipose

A conductive PEDOT/alginate porous scaffold as a platform to modulate the biological behaviors of brown adipose-derived stem cells

2020 ◽  
Vol 8 (11) ◽  
pp. 3173-3185 ◽  
Author(s):  
Boguang Yang ◽  
Fanglian Yao ◽  
Lei Ye ◽  
Tong Hao ◽  
Yabin Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cardiac Tissue ◽  
Porous Scaffold ◽  
Three Dimensional ◽  
Cardiac Tissue Engineering ◽  
Adipose Derived Stem Cells ◽  
Brown Adipose ◽  
Myocardial Differentiation

The development of three-dimensional conductive scaffolds is vital to support the adhesion, proliferation and myocardial differentiation of stem cells in cardiac tissue engineering.

scholarly journals C-type Natriuretic Peptide Promotes Adipogenic Differentiation of Goat Adipose Derived Stem Cells via cGMP/PKG/ p38 MAPK Signal Pathway

2020 ◽  
Author(s):  
Mingzhen Fan ◽  
Yutong Yan ◽  
Yuyang Miao ◽  
Ying Zhao ◽  
Ziqing Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
P38 Mapk ◽  
Natriuretic Peptide ◽  
Adipogenic Differentiation ◽  
Signal Pathway ◽  
Adipose Derived Stem Cells ◽  
Brown Adipose ◽  
Adipose Differentiation ◽  
Mapk Signal Pathway

Abstract BackgroundTwo types of adipose tissue, white adipose and brown adipose, have been identified in mammals. For goat, adipose tissue also plays an important role in improving meat and milk quality. C-type natriuretic peptide (CNP) is a member of natriuretic peptide family. Once CNP binds to natriuretic peptide receptor B (NPR-B), NPR-B induces the production of cGMP, thereby activating PKG and downstream targets. The expression of CNP and NPR-B in adipose tissue led to a hypothesis that CNP could have roles involving in regulation of adipogenesis in goat. However, there are few studies on the relationship between CNP and adipogenesis in goat. In the present study, goat adipose derived stem cells (ADSCs) were isolated and employed to investigate the effect of CNP on adipogenesis in goat.ResultsDuring adipogenic differentiation in goat ADSCs, the expression of NPR-B increased at 4 d and 8 d and reduced at 12 d after differentiation. When goat ADSCs were treated with 100 nM and 1000 nM CNP during adipogenic differentiation, there are more accumulation of lipid droplets in the cytoplasm and the up-regulation in the expression of adipogenesis relative genes including PPAR-γ, FASN and LPL. Interestingly, the expression level of brown adipose genes UCP-1 and PGC-1α were also up-regulated. Furthermore, the cGMP level was increased after treatment with CNP. Adding cGMP analogues 8-pCPT-cGMP (PKG activator) could increase adipogenesis efficiency, and adding both PKG inhibitor Rp-8-CPT-cGMP and CNP inhibited adipogenic differentiation. After treatment with CNP or PKG activator, the phosphorylation of p38 MAPK, MK2 and ATF2 were up-regulated, and their phosphorylation were significantly inhibited when CNP and PKG inhibitor were added simultaneously. SB203580 is the specific inhibitor for p38 MAPK. After treatment with CNP and SB203580 simultaneously, the adipogenic differentiation efficiency of goat ADSCs was significantly inhibited, and the expression of UCP-1 and PGC-1α were also reduced. ConclusionsCNP could promote adipogenic differentiation of goat ADSCs. The stimulative effect of CNP on adipose differentiation depends on the cGMP/PKG/ p38 MAPK signal pathway. Our study will be helpful to understand the regulation mechanism of goat adipose differentiation, especially brown adipose differentiation.

Tbx18-dependent differentiation of brown adipose tissue-derived stem cells toward cardiac pacemaker cells

2017 ◽  
Vol 433 (1-2) ◽  
pp. 61-77 ◽  
Author(s):  
Lei Chen ◽  
Zi-Jun Deng ◽  
Jian-Sheng Zhou ◽  
Rui-Juan Ji ◽  
Xi Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Cardiac Pacemaker ◽  
Pacemaker Cells ◽  
Brown Adipose

scholarly journals Metabolically Active Human Brown Adipose Tissue Derived Stem Cells

Stem Cells ◽  
2014 ◽  
Vol 32 (2) ◽  
pp. 572-581 ◽  
Author(s):  
Francisco J Silva ◽  
Dolly J Holt ◽  
Vanessa Vargas ◽  
James Yockman ◽  
Sihem Boudina ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Brown Adipose
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