scholarly journals Investigation of Postnatal Craniofacial Bone Development with Tissue Clearing-Based Three-Dimensional Imaging

2019 ◽  
Vol 28 (19) ◽  
pp. 1310-1321 ◽  
Author(s):  
Wenjing Luo ◽  
Yating Yi ◽  
Dian Jing ◽  
Shiwen Zhang ◽  
Yi Men ◽  
...  
Keyword(s):  
Bone Development ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Craniofacial Bone

scholarly journals Three-dimensional imaging of KNDy neurons in the mammalian brain using optical tissue clearing and multiple-label immunocytochemistry

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Aleisha M. Moore ◽  
Kathryn A. Lucas ◽  
Robert L. Goodman ◽  
Lique M. Coolen ◽  
Michael N. Lehman
Keyword(s):  
Three Dimensional ◽  
Mammalian Brain ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Kndy Neurons

Tissue clearing‐based method for unobstructed three‐dimensional imaging of mouse penis with subcellular resolution

2020 ◽  
Vol 13 (7) ◽  
Author(s):  
Paweł Matryba ◽  
Artur Wolny ◽  
Monika Pawłowska ◽  
Anna Sosnowska ◽  
Zuzanna Rydzyńska ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Subcellular Resolution

scholarly journals Three-dimensional imaging of intact porcine cochlea using tissue clearing and custom-built light-sheet microscopy

2020 ◽  
Vol 11 (11) ◽  
pp. 6181
Author(s):  
Adele Moatti ◽  
Yuheng Cai ◽  
Chen Li ◽  
Tyler Sattler ◽  
Laura Edwards ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Light Sheet ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Light Sheet Microscopy

Three-Dimensional Imaging of Intraplaque Neovascularization in a Mouse Model of Advanced Atherosclerosis

2020 ◽  
Vol 57 (6) ◽  
pp. 348-354 ◽  
Author(s):  
Paola Perrotta ◽  
Isabel Pintelon ◽  
Margreet R. de Vries ◽  
Paul H.A. Quax ◽  
Jean-Pierre Timmermans ◽  
...  
Keyword(s):  
Mouse Model ◽  
Three Dimensional ◽  
Heterozygous Mutation ◽  
Plaque Morphology ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Dibenzyl Ether ◽  
Fibrillin 1 ◽  
Secondary Antibodies ◽  
Reliable Methods

Multiple lines of evidence suggest that intraplaque (IP) neovascularization promotes atherosclerotic plaque growth, destabilization, and rupture. However, pharmacological inhibition of IP neovascularization remains largely unexplored due to the limited number of animal models that develop IP neovessels and the lack of reliable methods for visualizing IP angiogenesis. Here, we applied 3D confocal microscopy with an optimized tissue-clearing process, immunolabeling-enabled three-dimensional imaging of solvent-cleared organs, to visualize IP neovessels in apolipoprotein E-deficient (ApoE<sup>−/−</sup>) mice carrying a heterozygous mutation (C1039+/−) in the fibrillin-1 gene. Unlike regular ApoE<sup>−/−</sup> mice, this mouse model is characterized by the presence of advanced plaques with evident IP neovascularization. Plaques were stained with antibodies against endothelial marker CD31 for 3 days, followed by incubation with fluorescently labeled secondary antibodies. Subsequent tissue clearing with dichloromethane (DCM)/methanol, DCM, and dibenzyl ether allowed easy visualization and 3D reconstruction of the IP vascular network while plaque morphology remained intact.

scholarly journals Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease

iScience ◽  
2020 ◽  
Vol 23 (8) ◽  
pp. 101432
Author(s):  
Maria Victoria Gómez-Gaviro ◽  
Daniel Sanderson ◽  
Jorge Ripoll ◽  
Manuel Desco
Keyword(s):  
Biomedical Applications ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Health And Disease

scholarly journals Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Kohei Togami ◽  
Hiroaki Ozaki ◽  
Yuki Yumita ◽  
Anri Kitayama ◽  
Hitoshi Tada ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Pulmonary Fibrosis ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Collagen I ◽  
Immunofluorescence Staining ◽  
Therapeutic Effects ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Tomato Lectin

Idiopathic pulmonary fibrosis is a progressive, chronic lung disease characterized by the accumulation of extracellular matrix proteins, including collagen and elastin. Imaging of extracellular matrix in fibrotic lungs is important for evaluating its pathological condition as well as the distribution of drugs to pulmonary focus sites and their therapeutic effects. In this study, we compared techniques of staining the extracellular matrix with optical tissue-clearing treatment for developing three-dimensional imaging methods for focus sites in pulmonary fibrosis. Mouse models of pulmonary fibrosis were prepared via the intrapulmonary administration of bleomycin. Fluorescent-labeled tomato lectin, collagen I antibody, and Col-F, which is a fluorescent probe for collagen and elastin, were used to compare the imaging of fibrotic foci in intact fibrotic lungs. These lung samples were cleared using the ClearT2 tissue-clearing technique. The cleared lungs were two dimensionally observed using laser-scanning confocal microscopy, and the images were compared with those of the lung tissue sections. Moreover, three-dimensional images were reconstructed from serial two-dimensional images. Fluorescent-labeled tomato lectin did not enable the visualization of fibrotic foci in cleared fibrotic lungs. Although collagen I in fibrotic lungs could be visualized via immunofluorescence staining, collagen I was clearly visible only until 40 μm from the lung surface. Col-F staining facilitated the visualization of collagen and elastin to a depth of 120 μm in cleared lung tissues. Furthermore, we visualized the three-dimensional extracellular matrix in cleared fibrotic lungs using Col-F, and the images provided better visualization than immunofluorescence staining. These results suggest that ClearT2 tissue-clearing treatment combined with Col-F staining represents a simple and rapid technique for imaging fibrotic foci in intact fibrotic lungs. This study provides important information for imaging various organs with extracellular matrix-related diseases.

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