Rapid optical tissue clearing using poly(acrylamide‐co‐styrenesulfonate) hydrogels for three‐dimensional imaging

2019 ◽  
Vol 107 (7) ◽  
pp. 2297-2304 ◽  
Author(s):  
Yuta Ono ◽  
Ikuhiko Nakase ◽  
Akikazu Matsumoto ◽  
Chie Kojima
Keyword(s):  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Poly Acrylamide

scholarly journals Three-dimensional imaging of KNDy neurons in the mammalian brain using optical tissue clearing and multiple-label immunocytochemistry

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Aleisha M. Moore ◽  
Kathryn A. Lucas ◽  
Robert L. Goodman ◽  
Lique M. Coolen ◽  
Michael N. Lehman
Keyword(s):  
Three Dimensional ◽  
Mammalian Brain ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Kndy Neurons

Tissue clearing‐based method for unobstructed three‐dimensional imaging of mouse penis with subcellular resolution

2020 ◽  
Vol 13 (7) ◽  
Author(s):  
Paweł Matryba ◽  
Artur Wolny ◽  
Monika Pawłowska ◽  
Anna Sosnowska ◽  
Zuzanna Rydzyńska ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Subcellular Resolution

Three-Dimensional Imaging of Single Molecules Solvated in Pores of Poly(acrylamide) Gels

Science ◽  
1996 ◽  
Vol 274 (5289) ◽  
pp. 966-968 ◽  
Author(s):  
Robert M. Dickson ◽  
D. J. Norris ◽  
Yih-Ling Tzeng ◽  
W. E. Moerner
Keyword(s):  
Single Molecules ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Poly Acrylamide

scholarly journals Three-dimensional imaging of intact porcine cochlea using tissue clearing and custom-built light-sheet microscopy

2020 ◽  
Vol 11 (11) ◽  
pp. 6181
Author(s):  
Adele Moatti ◽  
Yuheng Cai ◽  
Chen Li ◽  
Tyler Sattler ◽  
Laura Edwards ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Light Sheet ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Light Sheet Microscopy

Three-Dimensional Imaging of Intraplaque Neovascularization in a Mouse Model of Advanced Atherosclerosis

2020 ◽  
Vol 57 (6) ◽  
pp. 348-354 ◽  
Author(s):  
Paola Perrotta ◽  
Isabel Pintelon ◽  
Margreet R. de Vries ◽  
Paul H.A. Quax ◽  
Jean-Pierre Timmermans ◽  
...  
Keyword(s):  
Mouse Model ◽  
Three Dimensional ◽  
Heterozygous Mutation ◽  
Plaque Morphology ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Dibenzyl Ether ◽  
Fibrillin 1 ◽  
Secondary Antibodies ◽  
Reliable Methods

Multiple lines of evidence suggest that intraplaque (IP) neovascularization promotes atherosclerotic plaque growth, destabilization, and rupture. However, pharmacological inhibition of IP neovascularization remains largely unexplored due to the limited number of animal models that develop IP neovessels and the lack of reliable methods for visualizing IP angiogenesis. Here, we applied 3D confocal microscopy with an optimized tissue-clearing process, immunolabeling-enabled three-dimensional imaging of solvent-cleared organs, to visualize IP neovessels in apolipoprotein E-deficient (ApoE<sup>−/−</sup>) mice carrying a heterozygous mutation (C1039+/−) in the fibrillin-1 gene. Unlike regular ApoE<sup>−/−</sup> mice, this mouse model is characterized by the presence of advanced plaques with evident IP neovascularization. Plaques were stained with antibodies against endothelial marker CD31 for 3 days, followed by incubation with fluorescently labeled secondary antibodies. Subsequent tissue clearing with dichloromethane (DCM)/methanol, DCM, and dibenzyl ether allowed easy visualization and 3D reconstruction of the IP vascular network while plaque morphology remained intact.

scholarly journals Investigation of Postnatal Craniofacial Bone Development with Tissue Clearing-Based Three-Dimensional Imaging

2019 ◽  
Vol 28 (19) ◽  
pp. 1310-1321 ◽  
Author(s):  
Wenjing Luo ◽  
Yating Yi ◽  
Dian Jing ◽  
Shiwen Zhang ◽  
Yi Men ◽  
...  
Keyword(s):  
Bone Development ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Craniofacial Bone

scholarly journals Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease

iScience ◽  
2020 ◽  
Vol 23 (8) ◽  
pp. 101432
Author(s):  
Maria Victoria Gómez-Gaviro ◽  
Daniel Sanderson ◽  
Jorge Ripoll ◽  
Manuel Desco
Keyword(s):  
Biomedical Applications ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Health And Disease
Sign in / Sign up

Export Citation Format

Share Document