scholarly journals Three-dimensional imaging of intact porcine cochlea using tissue clearing and custom-built light-sheet microscopy

2020 ◽  
Vol 11 (11) ◽  
pp. 6181
Author(s):  
Adele Moatti ◽  
Yuheng Cai ◽  
Chen Li ◽  
Tyler Sattler ◽  
Laura Edwards ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Light Sheet ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Light Sheet Microscopy

scholarly journals Three-Dimensional Microscopy by Milling with Ultraviolet Excitation

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jiaming Guo ◽  
Camille Artur ◽  
Jason L. Eriksen ◽  
David Mayerich
Keyword(s):  
Three Dimensional ◽  
Light Sheet ◽  
Clinical Settings ◽  
Three Dimensional Imaging ◽  
Image Stack ◽  
Light Sheet Microscopy ◽  
Ultraviolet Excitation ◽  
Tissue Changes ◽  
Block Face ◽  
Core Facilities

Abstract Analysis of three-dimensional biological samples is critical to understanding tissue function and the mechanisms of disease. Many chronic conditions, like neurodegenerative diseases and cancers, correlate with complex tissue changes that are difficult to explore using two-dimensional histology. While three-dimensional techniques such as confocal and light-sheet microscopy are well-established, they are time consuming, require expensive instrumentation, and are limited to small tissue volumes. Three-dimensional microscopy is therefore impractical in clinical settings and often limited to core facilities at major research institutions. There would be a tremendous benefit to providing clinicians and researchers with the ability to routinely image large three-dimensional tissue volumes at cellular resolution. In this paper, we propose an imaging methodology that enables fast and inexpensive three-dimensional imaging that can be readily integrated into current histology pipelines. This method relies on block-face imaging of paraffin-embedded samples using deep-ultraviolet excitation. The imaged surface is then ablated to reveal the next tissue section for imaging. The final image stack is then aligned and reconstructed to provide tissue models that exceed the depth and resolution achievable with modern three-dimensional imaging systems.

scholarly journals Quantifying three-dimensional rodent retina vascular development using optical tissue clearing and light-sheet microscopy

2017 ◽  
Vol 22 (07) ◽  
pp. 1 ◽  
Author(s):  
Jasmine N. Singh ◽  
Taylor M. Nowlin ◽  
Gregory J. Seedorf ◽  
Steven H. Abman ◽  
Douglas P. Shepherd
Keyword(s):  
Vascular Development ◽  
Three Dimensional ◽  
Light Sheet ◽  
Tissue Clearing ◽  
Light Sheet Microscopy

scholarly journals Three-dimensional quantification of twisting in the Arabidopsis petiole

2021 ◽  
Author(s):  
Yuta Otsuka ◽  
Hirokazu Tsukaya
Keyword(s):  
Cross Sections ◽  
Three Dimensional ◽  
Light Sheet ◽  
Underlying Mechanism ◽  
Two Dimensions ◽  
Observation Angle ◽  
Differential Growth ◽  
Light Sheet Microscopy ◽  
Definition Of ◽  
Twisting Angle

AbstractOrganisms have a variety of three-dimensional (3D) structures that change over time. These changes include twisting, which is 3D deformation that cannot happen in two dimensions. Twisting is linked to important adaptive functions of organs, such as adjusting the orientation of leaves and flowers in plants to align with environmental stimuli (e.g. light, gravity). Despite its importance, the underlying mechanism for twisting remains to be determined, partly because there is no rigorous method for quantifying the twisting of plant organs. Conventional studies have relied on approximate measurements of the twisting angle in 2D, with arbitrary choices of observation angle. Here, we present the first rigorous quantification of the 3D twisting angles of Arabidopsis petioles based on light sheet microscopy. Mathematical separation of bending and twisting with strict definition of petiole cross-sections were implemented; differences in the spatial distribution of bending and twisting were detected via the quantification of angles along the petiole. Based on the measured values, we discuss that minute degrees of differential growth can result in pronounced twisting in petioles.

scholarly journals Three-Dimensional Cross-Sectional Light-Sheet Microscopy Imaging of the Inflamed Mouse Gut

2017 ◽  
Vol 153 (4) ◽  
pp. 898-900 ◽  
Author(s):  
Sebastian Zundler ◽  
Anika Klingberg ◽  
Daniela Schillinger ◽  
Sarah Fischer ◽  
Clemens Neufert ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Light Sheet ◽  
Cross Sectional ◽  
Microscopy Imaging ◽  
Light Sheet Microscopy

scholarly journals Three Dimensional Fluorescence Imaging Using Multiple Light-Sheet Microscopy

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e96551 ◽  
Author(s):  
Kavya Mohan ◽  
Subhajit B. Purnapatra ◽  
Partha Pratim Mondal
Keyword(s):  
Fluorescence Imaging ◽  
Three Dimensional ◽  
Light Sheet ◽  
Light Sheet Microscopy

scholarly journals Actin-based protrusions of migrating neutrophils are intrinsically lamellar and facilitate direction changes

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Lillian K Fritz-Laylin ◽  
Megan Riel-Mehan ◽  
Bi-Chang Chen ◽  
Samuel J Lord ◽  
Thomas D Goddard ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Light Sheet ◽  
Time Lapse ◽  
Three Dimensions ◽  
Cortical Actin ◽  
Linear Arrangement ◽  
Collagen Matrices ◽  
Flat Surfaces ◽  
Actin Networks ◽  
Light Sheet Microscopy

Leukocytes and other amoeboid cells change shape as they move, forming highly dynamic, actin-filled pseudopods. Although we understand much about the architecture and dynamics of thin lamellipodia made by slow-moving cells on flat surfaces, conventional light microscopy lacks the spatial and temporal resolution required to track complex pseudopods of cells moving in three dimensions. We therefore employed lattice light sheet microscopy to perform three-dimensional, time-lapse imaging of neutrophil-like HL-60 cells crawling through collagen matrices. To analyze three-dimensional pseudopods we: (i) developed fluorescent probe combinations that distinguish cortical actin from dynamic, pseudopod-forming actin networks, and (ii) adapted molecular visualization tools from structural biology to render and analyze complex cell surfaces. Surprisingly, three-dimensional pseudopods turn out to be composed of thin (<0.75 µm), flat sheets that sometimes interleave to form rosettes. Their laminar nature is not templated by an external surface, but likely reflects a linear arrangement of regulatory molecules. Although we find that Arp2/3-dependent pseudopods are dispensable for three-dimensional locomotion, their elimination dramatically decreases the frequency of cell turning, and pseudopod dynamics increase when cells change direction, highlighting the important role pseudopods play in pathfinding.

scholarly journals Three-dimensional imaging of KNDy neurons in the mammalian brain using optical tissue clearing and multiple-label immunocytochemistry

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Aleisha M. Moore ◽  
Kathryn A. Lucas ◽  
Robert L. Goodman ◽  
Lique M. Coolen ◽  
Michael N. Lehman
Keyword(s):  
Three Dimensional ◽  
Mammalian Brain ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Kndy Neurons

scholarly journals Whole-Brain Profiling of Cells and Circuits in Mammals by Tissue Clearing and Light-Sheet Microscopy

Neuron ◽  
2020 ◽  
Vol 106 (3) ◽  
pp. 369-387 ◽  
Author(s):  
Hiroki R. Ueda ◽  
Hans-Ulrich Dodt ◽  
Pavel Osten ◽  
Michael N. Economo ◽  
Jayaram Chandrashekar ◽  
...  
Keyword(s):  
Light Sheet ◽  
Whole Brain ◽  
Tissue Clearing ◽  
Light Sheet Microscopy
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