Developing a Cost-Effective and Scalable Production of Human Hepatic Competent Endoderm from Size-Controlled Pluripotent Stem Cell Aggregates

2018 ◽  
Vol 27 (4) ◽  
pp. 262-274 ◽  
Author(s):  
Zahra Farzaneh ◽  
Mostafa Najarasl ◽  
Saeed Abbasalizadeh ◽  
Massoud Vosough ◽  
Hossein Baharvand
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cost Effective ◽  
Cell Aggregates ◽  
Size Controlled ◽  
Scalable Production

Autofluorescence imaging for human pluripotent stem cell differentiation into cardiomyocytes

2021 ◽  
Author(s):  
Tongcheng Qian ◽  
Tiffany M. Heaster ◽  
Melissa C. Skala
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cost Effective ◽  
Stem Cell Differentiation ◽  
Autofluorescence Imaging ◽  
Label Free ◽  
Human Pluripotent Stem Cell ◽  
Non Invasive ◽  
Cardiovascular Patients ◽  
Density Concentration

Abstract Human pluripotent stem cell (hPSC)-derived cardiomyocytes provide a promising regenerative cell therapy for cardiovascular patients and an important model system to accelerate drug discovery. However, cost-effective and time-efficient platforms must be developed to evaluate the quality of hPSC-derived cardiomyocytes during biomanufacturing. Here, we develop a non-invasive label-free live cell imaging platform to predict the efficiency of hPSC differentiation into cardiomyocytes. Autofluorescence imaging of metabolic co-enzymes is performed under varying differentiation conditions (cell density, concentration of Wnt signaling activator) across three hPSC lines.

scholarly journals Cost-Effective Master Cell Bank Validation of Multiple Clinical-Grade Human Pluripotent Stem Cell Lines From a Single Donor

2014 ◽  
Vol 3 (10) ◽  
pp. 1116-1124 ◽  
Author(s):  
Liani Devito ◽  
Anastasia Petrova ◽  
Cristian Miere ◽  
Stefano Codognotto ◽  
Nicola Blakely ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Cost Effective ◽  
Clinical Grade ◽  
Human Pluripotent Stem Cell ◽  
Cell Bank ◽  
Single Donor ◽  
Master Cell Bank ◽  
Stem Cell Lines

Cryopreservation of pluripotent stem cell aggregates in defined protein-free formulation

2012 ◽  
Vol 29 (1) ◽  
pp. 143-153 ◽  
Author(s):  
Sébastien Sart ◽  
Teng Ma ◽  
Yan Li
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cell Aggregates
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