scholarly journals Human pluripotent stem cell-derived products: Advances towards robust, scalable and cost-effective manufacturing strategies

2014 ◽  
Vol 10 (1) ◽  
pp. 83-95 ◽  
Author(s):  
Michael J. Jenkins ◽  
Suzanne S. Farid
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cost Effective ◽  
Human Pluripotent Stem Cell

Autofluorescence imaging for human pluripotent stem cell differentiation into cardiomyocytes

2021 ◽  
Author(s):  
Tongcheng Qian ◽  
Tiffany M. Heaster ◽  
Melissa C. Skala
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cost Effective ◽  
Stem Cell Differentiation ◽  
Autofluorescence Imaging ◽  
Label Free ◽  
Human Pluripotent Stem Cell ◽  
Non Invasive ◽  
Cardiovascular Patients ◽  
Density Concentration

Abstract Human pluripotent stem cell (hPSC)-derived cardiomyocytes provide a promising regenerative cell therapy for cardiovascular patients and an important model system to accelerate drug discovery. However, cost-effective and time-efficient platforms must be developed to evaluate the quality of hPSC-derived cardiomyocytes during biomanufacturing. Here, we develop a non-invasive label-free live cell imaging platform to predict the efficiency of hPSC differentiation into cardiomyocytes. Autofluorescence imaging of metabolic co-enzymes is performed under varying differentiation conditions (cell density, concentration of Wnt signaling activator) across three hPSC lines.

scholarly journals Cost-Effective Master Cell Bank Validation of Multiple Clinical-Grade Human Pluripotent Stem Cell Lines From a Single Donor

2014 ◽  
Vol 3 (10) ◽  
pp. 1116-1124 ◽  
Author(s):  
Liani Devito ◽  
Anastasia Petrova ◽  
Cristian Miere ◽  
Stefano Codognotto ◽  
Nicola Blakely ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Cost Effective ◽  
Clinical Grade ◽  
Human Pluripotent Stem Cell ◽  
Cell Bank ◽  
Single Donor ◽  
Master Cell Bank ◽  
Stem Cell Lines

Regenerative potential of human pluripotent stem cell-derived MSCs in a Gunn rat liver injury model

2015 ◽  
Vol 53 (12) ◽  
Author(s):  
LS Spitzhorn ◽  
M Megges ◽  
C Kordes ◽  
I Sawitza ◽  
S Götze ◽  
...  
Keyword(s):  
Stem Cell ◽  
Liver Injury ◽  
Rat Liver ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Gunn Rat ◽  
Injury Model

Generation of expandable human pluripotent stem cell-derived hepatocyte-like liver organoids

2019 ◽  
Vol 71 (5) ◽  
pp. 970-985 ◽  
Author(s):  
Seon Ju Mun ◽  
Jae-Sung Ryu ◽  
Mi-Ok Lee ◽  
Ye Seul Son ◽  
Soo Jin Oh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Liver Organoids
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