Production of homogenous size‐controlled human induced pluripotent stem cell aggregates using ring‐shaped culture vessel

2021 ◽  
Author(s):  
Fuad Gandhi Torizal ◽  
Seong Min Kim ◽  
Ikki Horiguchi ◽  
Kousuke Inamura ◽  
Ikumi Suzuki ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Culture Vessel ◽  
Cell Aggregates ◽  
Induced Pluripotent ◽  
Size Controlled

scholarly journals Cryopreservation of Induced Pluripotent Stem Cell-Derived Dopaminergic Neurospheres for Clinical Application

2021 ◽  
pp. 1-14
Author(s):  
Satoe Hiramatsu ◽  
Asuka Morizane ◽  
Tetsuhiro Kikuchi ◽  
Daisuke Doi ◽  
Kenji Yoshida ◽  
...  
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Clinical Application ◽  
Pluripotent Stem Cell ◽  
3D Structure ◽  
Induced Pluripotent Stem Cell ◽  
Cell Aggregates ◽  
Induced Pluripotent

Background: Pluripotent stem cell (PSC)-derived dopaminergic (DA) neurons are an expected source of cell therapy for Parkinson’s disease. The transplantation of cell aggregates or neurospheres, instead of a single cell suspension has several advantages, such as keeping the 3D structure of the donor cells and ease of handling. For this PSC-based therapy to become a widely available treatment, cryopreservation of the final product is critical in the manufacturing process. However, cryopreserving cell aggregates is more complicated than cryopreserving single cell suspensions. Previous studies showed poor survival of the DA neurons after the transplantation of cryopreserved fetal ventral-mesencephalic tissues. Objective: To achieve the cryopreservation of induced pluripotent stem cell (iPSC)-derived DA neurospheres toward clinical application. Methods: We cryopreserved iPSC-derived DA neurospheres in various clinically applicable cryopreservation media and freezing protocols and assessed viability and neurite extension. We evaluated the population and neuronal function of cryopreserved cells by the selected method in vitro. We also injected the cells into 6-hydroxydopamine (6-OHDA) lesioned rats, and assessed their survival, maturation and function in vivo. Results: The iPSC-derived DA neurospheres cryopreserved by Proton Freezer in the cryopreservation medium Bambanker hRM (BBK) showed favorable viability after thawing and had equivalent expression of DA-specific markers, dopamine secretion, and electrophysiological activity as fresh spheres. When transplanted into 6-OHDA-lesioned rats, the cryopreserved cells survived and differentiated into mature DA neurons, resulting in improved abnormal rotational behavior. Conclusion: These results show that the combination of BBK and Proton Freezer is suitable for the cryopreservation of iPSC-derived DA neurospheres.

Expansion of 3D human induced pluripotent stem cell aggregates in bioreactors: Bioprocess intensification and scaling-up approaches

2017 ◽  
Vol 246 ◽  
pp. 81-93 ◽  
Author(s):  
Bernardo Abecasis ◽  
Tiago Aguiar ◽  
Émilie Arnault ◽  
Rita Costa ◽  
Patricia Gomes-Alves ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Scaling Up ◽  
Cell Aggregates ◽  
Induced Pluripotent

Use of 3D culture systems to generate human induced pluripotent stem cell-derived [beta]-cells in vitro

2018 ◽  
Author(s):  
Fantuzzi Federica ◽  
Toivonen Sanna ◽  
Schiavo Andrea Alex ◽  
Pachera Nathalie ◽  
Rajaei Bahareh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Beta Cells ◽  
Pluripotent Stem Cell ◽  
3D Culture ◽  
Induced Pluripotent Stem Cell ◽  
Culture Systems ◽  
Induced Pluripotent
Sign in / Sign up

Export Citation Format

Share Document