Cryopreservation of pluripotent stem cell aggregates in defined protein-free formulation

2012 ◽  
Vol 29 (1) ◽  
pp. 143-153 ◽  
Author(s):  
Sébastien Sart ◽  
Teng Ma ◽  
Yan Li
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cell Aggregates

Developing a Cost-Effective and Scalable Production of Human Hepatic Competent Endoderm from Size-Controlled Pluripotent Stem Cell Aggregates

2018 ◽  
Vol 27 (4) ◽  
pp. 262-274 ◽  
Author(s):  
Zahra Farzaneh ◽  
Mostafa Najarasl ◽  
Saeed Abbasalizadeh ◽  
Massoud Vosough ◽  
Hossein Baharvand
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cost Effective ◽  
Cell Aggregates ◽  
Size Controlled ◽  
Scalable Production

scholarly journals Cryopreservation of Induced Pluripotent Stem Cell-Derived Dopaminergic Neurospheres for Clinical Application

2021 ◽  
pp. 1-14
Author(s):  
Satoe Hiramatsu ◽  
Asuka Morizane ◽  
Tetsuhiro Kikuchi ◽  
Daisuke Doi ◽  
Kenji Yoshida ◽  
...  
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Clinical Application ◽  
Pluripotent Stem Cell ◽  
3D Structure ◽  
Induced Pluripotent Stem Cell ◽  
Cell Aggregates ◽  
Induced Pluripotent

Background: Pluripotent stem cell (PSC)-derived dopaminergic (DA) neurons are an expected source of cell therapy for Parkinson’s disease. The transplantation of cell aggregates or neurospheres, instead of a single cell suspension has several advantages, such as keeping the 3D structure of the donor cells and ease of handling. For this PSC-based therapy to become a widely available treatment, cryopreservation of the final product is critical in the manufacturing process. However, cryopreserving cell aggregates is more complicated than cryopreserving single cell suspensions. Previous studies showed poor survival of the DA neurons after the transplantation of cryopreserved fetal ventral-mesencephalic tissues. Objective: To achieve the cryopreservation of induced pluripotent stem cell (iPSC)-derived DA neurospheres toward clinical application. Methods: We cryopreserved iPSC-derived DA neurospheres in various clinically applicable cryopreservation media and freezing protocols and assessed viability and neurite extension. We evaluated the population and neuronal function of cryopreserved cells by the selected method in vitro. We also injected the cells into 6-hydroxydopamine (6-OHDA) lesioned rats, and assessed their survival, maturation and function in vivo. Results: The iPSC-derived DA neurospheres cryopreserved by Proton Freezer in the cryopreservation medium Bambanker hRM (BBK) showed favorable viability after thawing and had equivalent expression of DA-specific markers, dopamine secretion, and electrophysiological activity as fresh spheres. When transplanted into 6-OHDA-lesioned rats, the cryopreserved cells survived and differentiated into mature DA neurons, resulting in improved abnormal rotational behavior. Conclusion: These results show that the combination of BBK and Proton Freezer is suitable for the cryopreservation of iPSC-derived DA neurospheres.

scholarly journals Incorporation of Retinoic Acid Releasing Microspheres into Pluripotent Stem Cell Aggregates for Inducing Neuronal Differentiation

2015 ◽  
Vol 8 (3) ◽  
pp. 307-319 ◽  
Author(s):  
Jose Carlos Gomez ◽  
John M. Edgar ◽  
Andrew M. Agbay ◽  
Emma Bibault ◽  
Amy Montgomery ◽  
...  
Keyword(s):  
Stem Cell ◽  
Retinoic Acid ◽  
Neuronal Differentiation ◽  
Pluripotent Stem Cell ◽  
Cell Aggregates

scholarly journals A microparticle approach to morphogen delivery within pluripotent stem cell aggregates

Biomaterials ◽  
2013 ◽  
Vol 34 (30) ◽  
pp. 7227-7235 ◽  
Author(s):  
Andrés M. Bratt-Leal ◽  
Anh H. Nguyen ◽  
Katy A. Hammersmith ◽  
Ankur Singh ◽  
Todd C. McDevitt
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cell Aggregates
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