scholarly journals Global Transcriptional Profiling of Neural and Mesenchymal Progenitors Derived from Human Embryonic Stem Cells Reveals Alternative Developmental Signaling Pathways

2011 ◽  
Vol 20 (8) ◽  
pp. 1395-1409 ◽  
Author(s):  
Jérôme Alexandre Denis ◽  
Christelle Rochon-Beaucourt ◽  
Benoite Champon ◽  
Geneviève Pietu
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathways ◽  
Human Embryonic Stem Cells ◽  
Transcriptional Profiling ◽  
Embryonic Stem ◽  
Mesenchymal Progenitors ◽  
Developmental Signaling

Transcriptional profiling of initial differentiation events in human embryonic stem cells

2004 ◽  
Vol 323 (2) ◽  
pp. 453-464 ◽  
Author(s):  
John D. Calhoun ◽  
Raj R. Rao ◽  
Susanne Warrenfeltz ◽  
Romdhane Rekaya ◽  
Stephen Dalton ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Transcriptional Profiling ◽  
Embryonic Stem

CDK11 safeguards the identity of human embryonic stem cells via fine‐tuning signaling pathways

2019 ◽  
Vol 235 (5) ◽  
pp. 4279-4290
Author(s):  
Jianyi Ding ◽  
Zhuoqing Fang ◽  
Xinyuan Liu ◽  
Zhexin Zhu ◽  
Chunsheng Wen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathways ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Fine Tuning

scholarly journals Expression profiles of protein tyrosine kinase genes in human embryonic stem cells

Reproduction ◽  
2008 ◽  
Vol 136 (4) ◽  
pp. 423-432 ◽  
Author(s):  
Mi-Young Son ◽  
Janghwan Kim ◽  
Hyo-Won Han ◽  
Sun-Mi Woo ◽  
Yee Sook Cho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathways ◽  
Human Embryonic Stem Cells ◽  
Tyrosine Kinases ◽  
Expression Profiles ◽  
Embryonic Stem ◽  
Degenerate Primers ◽  
Protein Tyrosine ◽  
Hesc Lines

Complex signaling pathways operate in human embryonic stem cells (hESCs) and are coordinated to maintain self-renewal and stem cell characteristics in them. Protein tyrosine kinases (PTKs) participate in diverse signaling pathways in various types of cells. Because of their functions as key molecules in various cellular processes, PTKs are anticipated to have important roles also in hESCs. In this study, we investigated the roles of PTKs in undifferentiated and differentiated hESCs. To establish comprehensive PTK expression profiles in hESCs, we performed reverse transcriptase PCR using degenerate primers according to the conserved catalytic PTK motifs in both undifferentiated and differentiated hESCs. Here, we identified 42 different kinases in two hESC lines, including 5 non-receptor tyrosine kinases (RTKs), 24 RTKs, and 13 dual and other kinases, and compared the protein kinase expression profiles of hESCs and retinoic acid-treated hESCs. Significantly, up- and downregulated kinases in undifferentiated hESCs were confirmed by real-time PCR and western blotting. MAP3K3, ERBB2, FGFR4, and EPHB2 were predominantly upregulated, while CSF1R, TYRO3, SRC, and GSK3A were consistently downregulated in two hESC lines. Western blot analysis showed that the transcriptional levels of these kinases were consistent with the translational levels. The obstruction of upregulated kinases’ activities using specific inhibitors disturbed the undifferentiated status and induced the differentiation of hESCs. Our results support the dynamic expression of PTKs during hESC maintenance and suggest that specific PTKs that are consistently up- and downregulated play important roles in the maintenance of stemness and the direction of differentiation of hESCs.

scholarly journals Transcriptional profiling of the developmentally important signalling pathways in human embryonic stem cells

2005 ◽  
Vol 21 (2) ◽  
pp. 405-412 ◽  
Author(s):  
Jeung-Yon Rho ◽  
Kweon Yu ◽  
Jee-Soo Han ◽  
Jung-Il Chae ◽  
Deog-Bon Koo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Transcriptional Profiling ◽  
Embryonic Stem ◽  
Signalling Pathways

Genome-Wide Transcriptional Profiling of Human Embryonic Stem Cells Differentiating to Cardiomyocytes

Stem Cells ◽  
2006 ◽  
Vol 24 (8) ◽  
pp. 1956-1967 ◽  
Author(s):  
Abdelaziz Beqqali ◽  
Jantine Kloots ◽  
Dorien Ward-van Oostwaard ◽  
Christine Mummery ◽  
Robert Passier
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Transcriptional Profiling ◽  
Embryonic Stem ◽  
Genome Wide

scholarly journals Transcriptional Profiling of Ectoderm Specification to Keratinocyte Fate in Human Embryonic Stem Cells

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0122493 ◽  
Author(s):  
Ana Mafalda Baptista Tadeu ◽  
Samantha Lin ◽  
Lin Hou ◽  
Lisa Chung ◽  
Mei Zhong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Transcriptional Profiling ◽  
Embryonic Stem
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