Enhanced differentiation of human embryonic stem cells to mesenchymal progenitors by inhibition of TGF-β/activin/nodal signaling using SB-431542

2010 ◽  
Vol 25 (6) ◽  
pp. 1216-1233 ◽  
Author(s):  
Amer Mahmood ◽  
Linda Harkness ◽  
Henrik Daa Schrøder ◽  
Basem M Abdallah ◽  
Moustapha Kassem
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Nodal Signaling ◽  
Mesenchymal Progenitors

scholarly journals Activin/Nodal Signaling Controls Divergent Transcriptional Networks in Human Embryonic Stem Cells and in Endoderm Progenitors

Stem Cells ◽  
2011 ◽  
Vol 29 (8) ◽  
pp. 1176-1185 ◽  
Author(s):  
Stephanie Brown ◽  
Adrian Teo ◽  
Siim Pauklin ◽  
Nicholas Hannan ◽  
Candy H.-H. Cho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Transcriptional Networks ◽  
Nodal Signaling

scholarly journals Inhibition of Activin/Nodal signaling promotes specification of human embryonic stem cells into neuroectoderm

2008 ◽  
Vol 313 (1) ◽  
pp. 107-117 ◽  
Author(s):  
Joseph R. Smith ◽  
Ludovic Vallier ◽  
Giuseppe Lupo ◽  
Morgan Alexander ◽  
William A. Harris ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Nodal Signaling

scholarly journals Rapid changes in morphogen concentration control self-organized patterning in human embryonic stem cells

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Idse Heemskerk ◽  
Kari Burt ◽  
Matthew Miller ◽  
Sapna Chhabra ◽  
M Cecilia Guerra ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Cell Fate ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Nodal Signaling ◽  
Cell Fate Decisions ◽  
Self Organized ◽  
Rapid Changes

During embryonic development, diffusible signaling molecules called morphogens are thought to determine cell fates in a concentration-dependent way. Yet, in mammalian embryos, concentrations change rapidly compared to the time for making cell fate decisions. Here, we use human embryonic stem cells (hESCs) to address how changing morphogen levels influence differentiation, focusing on how BMP4 and Nodal signaling govern the cell-fate decisions associated with gastrulation. We show that BMP4 response is concentration dependent, but that expression of many Nodal targets depends on rate of concentration change. Moreover, in a self-organized stem cell model for human gastrulation, expression of these genes follows rapid changes in endogenous Nodal signaling. Our study shows a striking contrast between the specific ways ligand dynamics are interpreted by two closely related signaling pathways, highlighting both the subtlety and importance of morphogen dynamics for understanding mammalian embryogenesis and designing optimized protocols for directed stem cell differentiation.Editorial note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see <xref ref-type="decision-letter" rid="SA1">decision letter</xref>).

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