Genome-Wide Transcriptional Profiling of Human Embryonic Stem Cells Differentiating to Cardiomyocytes

Stem Cells ◽  
2006 ◽  
Vol 24 (8) ◽  
pp. 1956-1967 ◽  
Author(s):  
Abdelaziz Beqqali ◽  
Jantine Kloots ◽  
Dorien Ward-van Oostwaard ◽  
Christine Mummery ◽  
Robert Passier
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Transcriptional Profiling ◽  
Embryonic Stem ◽  
Genome Wide

scholarly journals Genome-Wide Definition of Promoter and Enhancer Usage during Neural Induction of Human Embryonic Stem Cells

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0126590 ◽  
Author(s):  
Valentina Poletti ◽  
Alessia Delli Carri ◽  
Guidantonio Malagoli Tagliazucchi ◽  
Andrea Faedo ◽  
Luca Petiti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Neural Induction ◽  
Genome Wide ◽  
Wide Definition ◽  
Definition Of

Transcriptional profiling of initial differentiation events in human embryonic stem cells

2004 ◽  
Vol 323 (2) ◽  
pp. 453-464 ◽  
Author(s):  
John D. Calhoun ◽  
Raj R. Rao ◽  
Susanne Warrenfeltz ◽  
Romdhane Rekaya ◽  
Stephen Dalton ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Transcriptional Profiling ◽  
Embryonic Stem

scholarly journals TPX2 Amplification-Driven Aberrant Mitosis in Long-Term Cultured Human Embryonic Stem Cells

2021 ◽  
Author(s):  
Ho-Chang Jeong ◽  
Young-Hyun Go ◽  
Joong-Gon Shin ◽  
Yun-Jeong Kim ◽  
Min-Guk Cho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mitotic Progression ◽  
Genome Wide ◽  
Hesc Culture ◽  
Spindle Stability

AbstractAlthough human embryonic stem cells (hESCs) are equipped with highly effective machinery for the maintenance of genome integrity, the frequency of genetic aberrations during long-term in vitro hESC culture has been a serious issue that raises concerns over their safety in future clinical applications. By passaging hESCs over a broad range of timepoints, we found that mitotic aberrations, such as the delay of mitosis, multipolar centrosomes, and chromosome mis-segregation, were increased in the late-passaged hESCs (LP-hESCs) in parallel with polyploidy compared to early-passaged hESCs (EP-hESCs). Through high-resolution genome-wide approaches and by following transcriptome analysis, we found that LP-hESCs with a minimal amplicon in chromosome 20q11.21 highly expressed TPX2 (targeting protein for Xklp2), a key protein for governing spindle assembly and cancer malignancy. Consistent with these findings, the inducible expression of TPX2 in EP-hESCs reproduced aberrant mitotic events, such as the delay of mitotic progression, spindle stability, misaligned chromosomes, and polyploidy. This data suggests that the amplification and increased transcription of the TPX2 gene at 20q11.21 could contribute to an increase in aberrant mitosis due to altered spindle dynamics.

scholarly journals Transcriptional profiling of the developmentally important signalling pathways in human embryonic stem cells

2005 ◽  
Vol 21 (2) ◽  
pp. 405-412 ◽  
Author(s):  
Jeung-Yon Rho ◽  
Kweon Yu ◽  
Jee-Soo Han ◽  
Jung-Il Chae ◽  
Deog-Bon Koo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Transcriptional Profiling ◽  
Embryonic Stem ◽  
Signalling Pathways

scholarly journals Genome-wide dynamic changes of DNA methylation of repetitive elements in human embryonic stem cells and fetal fibroblasts

Genomics ◽  
2012 ◽  
Vol 99 (1) ◽  
pp. 10-17 ◽  
Author(s):  
Jianzhong Su ◽  
Xiujuan Shao ◽  
Hongbo Liu ◽  
Shengqiang Liu ◽  
Qiong Wu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Repetitive Elements ◽  
Dynamic Changes ◽  
Genome Wide ◽  
Fetal Fibroblasts

scholarly journals Use of human embryonic stem cells to model pediatric gliomas with H3.3K27M histone mutation

Science ◽  
2014 ◽  
Vol 346 (6216) ◽  
pp. 1529-1533 ◽  
Author(s):  
Kosuke Funato ◽  
Tamara Major ◽  
Peter W. Lewis ◽  
C. David Allis ◽  
Viviane Tabar
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Neural Progenitor Cells ◽  
Embryonic Stem ◽  
Cell System ◽  
Genome Wide ◽  
Regulator Genes

Over 70% of diffuse intrinsic pediatric gliomas, an aggressive brainstem tumor, harbor heterozygous mutations that create a K27M amino acid substitution (methionine replaces lysine 27) in the tail of histone H3.3. The role of the H3.3K27M mutation in tumorigenesis is not fully understood. Here, we use a human embryonic stem cell system to model this tumor. We show that H3.3K27M expression synergizes with p53 loss and PDGFRA activation in neural progenitor cells derived from human embryonic stem cells, resulting in neoplastic transformation. Genome-wide analyses indicate a resetting of the transformed precursors to a developmentally more primitive stem cell state, with evidence of major modifications of histone marks at several master regulator genes. Drug screening assays identified a compound targeting the protein menin as an inhibitor of tumor cell growth in vitro and in mice.

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