The Action of Ethyl Carbamate (Urethane) on the Respiration of Active and Blocked Embryonic Cells

1948 ◽  
Vol 21 (4) ◽  
pp. 303-308 ◽  
Author(s):  
Joseph Hall Bodine ◽  
Laurence Rockwell Fitzgerald
Keyword(s):  
Ethyl Carbamate ◽  
Embryonic Cells

Laser Scanning Confocal Microscopy and Three-Dimesnsional Reconstruction of the Mitotic Spindles of Unequally Dividing Embryonic Cells

1990 ◽  
Vol 48 (3) ◽  
pp. 166-167
Author(s):  
J. Holy ◽  
G. Schatten
Keyword(s):  
Confocal Microscopy ◽  
Mitotic Spindle ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Confocal Microscopy ◽  
Two Dimensions ◽  
Embryonic Cells ◽  
Mitotic Spindles ◽  
Cleavage Division ◽  
Scanning Confocal Microscopy

One of the classic limitations of light microscopy has been the fact that three dimensional biological events could only be visualized in two dimensions. Recently, this shortcoming has been overcome by combining the technologies of laser scanning confocal microscopy (LSCM) and computer processing of microscopical data by volume rendering methods. We have employed these techniques to examine morphogenetic events characterizing early development of sea urchin embryos. Specifically, the fourth cleavage division was examined because it is at this point that the first morphological signs of cell differentiation appear, manifested in the production of macromeres and micromeres by unequally dividing vegetal blastomeres.The mitotic spindle within vegetal blastomeres undergoing unequal cleavage are highly polarized and develop specialized, flattened asters toward the micromere pole. In order to reconstruct the three-dimensional features of these spindles, both isolated spindles and intact, extracted embryos were fluorescently labeled with antibodies directed against either centrosomes or tubulin.

TEMPERATURE DEPENDENCE OF ATP HYDROLYSIS BY LOACH EMBRYONIC CELLS Na+/K+-ATPase UNDER THE INFLUENCE OF He-Ne LASER

2016 ◽  
Vol 18 (2) ◽  
pp. 88-93
Author(s):  
M. S. Romaniuk ◽  
◽  
S. M. Mandzynets ◽  
M. V. Bura ◽  
D. I. Sanagursky ◽  
...  
Keyword(s):  
Temperature Dependence ◽  
Atp Hydrolysis ◽  
Embryonic Cells
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