Characterization of embryonic cells obtained from multifetal reduction

10.2741/e847 ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 79-88
Author(s):  
Nagwa El-Badri
Keyword(s):  
Embryonic Cells

scholarly journals Characterization of an insulin receptor-related receptor in Biomphalaria glabrata embryonic cells

2001 ◽  
Vol 1510 (1-2) ◽  
pp. 321-329 ◽  
Author(s):  
Vinca Lardans ◽  
Jean-François Coppin ◽  
Jérôme Vicogne ◽  
Eric Aroca ◽  
Melaine Delcroix ◽  
...  
Keyword(s):  
Insulin Receptor ◽  
Biomphalaria Glabrata ◽  
Embryonic Cells

scholarly journals Characterization of Mouse Embryonic Cells Deficient in the Ctr1 High Affinity Copper Transporter

2002 ◽  
Vol 277 (43) ◽  
pp. 40253-40259 ◽  
Author(s):  
Jaekwon Lee ◽  
Michael J. Petris ◽  
Dennis J. Thiele
Keyword(s):  
Embryonic Cells ◽  
Copper Transporter ◽  
High Affinity

scholarly journals The Maternal CCAAT Box Transcription Factor Which Controls GATA-2 Expression Is Novel and Developmentally Regulated and Contains a Double-Stranded-RNA-Binding Subunit

1998 ◽  
Vol 18 (9) ◽  
pp. 5557-5566 ◽  
Author(s):  
Robert L. Orford ◽  
Carl Robinson ◽  
Joanna M. Haydon ◽  
Roger K. Patient ◽  
Matthew J. Guille
Keyword(s):  
Transcription Factor ◽  
Rna Binding ◽  
Binding Activity ◽  
Embryonic Cells ◽  
Developmentally Regulated ◽  
Double Stranded Rna ◽  
Dna Binding Activity ◽  
Multistep Process ◽  
Ccaat Box

ABSTRACT The transcription factor GATA-2 is expressed at high levels in the nonneural ectoderm of the Xenopus embryo at neurula stages, with lower amounts of RNA present in the ventral mesoderm and endoderm. The promoter of the GATA-2 gene contains an inverted CCAAT box conserved among Xenopus laevis, humans, chickens, and mice. We have shown that this sequence is essential for GATA-2 transcription during early development and that the factor binding it is maternal. The DNA-binding activity of this factor is detectable in nuclei and chromatin bound only when zygotic GATA-2 transcription starts. Here we report the characterization of this factor, which we call CBTF (CCAAT box transcription factor). CBTF activity mainly appears late in oogenesis, when it is nuclear, and the complex has multiple subunits. We have identified one subunit of the factor as p122, aXenopus double-stranded-RNA-binding protein. The p122 protein is perinuclear during early embryonic development but moves from the cytoplasm into the nuclei of embryonic cells at stage 9, prior to the detection of CBTF activity in the nucleus. Thus, the accumulation of CBTF activity in the nucleus is a multistep process. We show that the p122 protein is expressed mainly in the ectoderm. Expression of p122 mRNA is more restricted, mainly to the anterior ectoderm and mesoderm and to the neural tube. Two properties of CBTF, its dual role and its cytoplasm-to-nucleus translocation, are shared with other vertebrate maternal transcription factors and may be general properties of these proteins.

scholarly journals Chimera-competent eXtra-Embryonic eNdoderm (XEN) cells established from pig embryos

2020 ◽  
Author(s):  
Chi Park ◽  
Young Jeoung ◽  
Jun Uh ◽  
Kieun Park ◽  
Jessica Bridge ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blastocyst Stage ◽  
Yolk Sac ◽  
Embryonic Cells ◽  
Wide Spread ◽  
Primitive Endoderm ◽  
Visceral Yolk Sac ◽  
First Time

AbstractIn this article, we report for the first time the derivation and characterization of extra-embryonic endoderm (XEN) cells from primitive endoderm (PrE) of porcine (p) embryos. The pXEN cells can be reliably and reproducibly generated from parthenote, in vitro and in vivo derived embryos. The pXEN cells retained all the hallmarks of PrE including expression of canonical PrE and XEN cell markers (GATA4, GATA6, SOX17, SALL4, FOXA2, and HNF4A). Transcriptome analysis further confirmed their XEN cell origin. The pXEN cells when introduced into blastocyst stage embryo contributed to wide-spread chimerism including visceral yolk sac, chorion, as well as embryonic gut and liver primordium in the fetus. The pXEN cells were shown to be an efficient nuclear donor for generating cloned offspring. Taken together, pXEN cells fulfil a longstanding need for a stable, chimera-competent, and nuclear transfer-compatible porcine embryonic cells with applications for agriculture and medicine.Significance StatementWe report for the first time, the derivation and characterization of extraembryonic endoderm (XEN) stem cells from porcine (p) embryos. The pXEN cells can be reliably and reproducibly derived from primitive endoderm precursors. When injected into blastocyst-stage embryos, the pXEN cells have contributed to wide-spread chimerism including visceral yolk sac, chorion of the extraembryonic membranes, as well as definitive endoderm of the fetus, primarily the embryonic gut and liver primordium. Additionally, these XEN cells have proven to be an efficient nuclear donor for generating cloned offspring. These newly discovered stem cells provide a novel model for studying lineage segregation, as well as a source for interspecies chimeras for generating endodermal organs, and for genome editing in livestock.

Organization of immunoglobulin genes

1978 ◽  
Vol 36 (2) ◽  
pp. 194-195
Author(s):  
CH. Brack ◽  
R. Lenhard-Schuller ◽  
A. Traunecker ◽  
S. Tonegawa
Keyword(s):  
Light Chain ◽  
Dna Fragments ◽  
Embryonic Cells ◽  
Loop Formation ◽  
Electron Microscopic ◽  
Embryonic Mouse ◽  
Myeloma Cells ◽  
Rearrangement Process ◽  
V Gene

Recent DNA-RNA hybridization experiments suggested that the genes coding for variable (V gene) and constant (C gene) parts of immunoglobulin molecules are separate in embryonic mouse DNA, whereas in lymphocyte DNA they are close together, implying a somatic rearrangement process of the two genes during lymphocyte differentiation. To study mechanisms involved in the organization and expression of Ig genes at a molecular level, we have been cloning DNA fragments containing Ig light chain genes from early embryonic cells and from myeloma cells. We here report electron microscopic characterization of three cloned DNA fragments: two clones from embryonic DNA carrying V gene (Ig 99) and C gene sequences (Ig 25), and one clone from myeloma DNA carrying both V and C genes (Ig 303).R-loop mapping. The purified DNA fragments were hybridized to Ig mRNA (isolated from λ type light chain producing myeloma cells) under conditions of R-loop formation. Measurement of the position and extension of the R-loops allowed us to localize the regions homologous to the Ig mRNA and to construct a preliminary map of the DNA fragments.

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