scholarly journals Microbiological Diagnosis of Empyema in Children: Comparative Evaluations by Culture, Polymerase Chain Reaction, and Pneumococcal Antigen Detection in Pleural Fluids

2006 ◽  
Vol 42 (8) ◽  
pp. 1135-1140 ◽  
Author(s):  
A. Le Monnier ◽  
E. Carbonnelle ◽  
J.-R. Zahar ◽  
M. Le Bourgeois ◽  
E. Abachin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Antigen Detection ◽  
Microbiological Diagnosis ◽  
Chain Reaction ◽  
Pneumococcal Antigen ◽  
Polymerase Chain

Microbiological Diagnosis of Sepsis: Polymerase Chain Reaction System Versus Blood Cultures

2015 ◽  
Vol 25 (1) ◽  
pp. 68-75 ◽  
Author(s):  
B. Suberviola ◽  
A. Marquez-Lopez ◽  
A. Castellanos-Ortega ◽  
C. Fernandez-Mazarrasa ◽  
M. Santibanez ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reaction System ◽  
Blood Cultures ◽  
Microbiological Diagnosis ◽  
Chain Reaction ◽  
Polymerase Chain Reaction System ◽  
System Versus ◽  
Polymerase Chain

Isolation of Ureaplasma urealyticum and Mycoplasma hominis From Public Toilet Bowls

1999 ◽  
Vol 20 (01) ◽  
pp. 66-68 ◽  
Author(s):  
Israel Potasman ◽  
Amnon Oren ◽  
Isaac Srugo
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Antigen Detection ◽  
Chain Reaction ◽  
Survival Assay ◽  
Polymerase Chain

Abstract The presence of Ureaplasma urealyticum, Mycoplasma hominis, and Chlamydia trachomatis was explored in 50 public restroom toilet bowls. We used culture, antigen detection, polymerase chain reaction, and survival assay. Five bowls (10%) were contaminated with at least one organism. U urealyticum was found in four bowls, M hominis in three, and C trachomatis in one. U urealyticum survived on the toilet rim for up to 2 hours.

Skin biopsy polymerase chain reaction for rapid microbiological diagnosis in patients with purpura fulminans

2017 ◽  
Vol 177 (4) ◽  
pp. e154-e155 ◽  
Author(s):  
T. de Risi-Pugliese ◽  
A. Servy ◽  
J.-W. Decousser ◽  
B. Nebbad ◽  
M. Desroches ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Skin Biopsy ◽  
Purpura Fulminans ◽  
Microbiological Diagnosis ◽  
Chain Reaction ◽  
Polymerase Chain

Comparison of Serum Galactomannan Antigen Detection and Competitive Polymerase Chain Reaction for Diagnosing Invasive Aspergillosis

1998 ◽  
Vol 26 (6) ◽  
pp. 1407-1412 ◽  
Author(s):  
Stephane Bretagne ◽  
Jean‐Marc Costa ◽  
Emmanuelle Bart–Delabesse ◽  
Nathalie Dhedin ◽  
Claire Rieux ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Invasive Aspergillosis ◽  
Antigen Detection ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Galactomannan Antigen ◽  
Polymerase Chain

scholarly journals Head-to-Head Comparison of Rapid and Automated Antigen Detection Tests for the Diagnosis of SARS-CoV-2 Infection

2021 ◽  
Vol 10 (2) ◽  
pp. 265
Author(s):  
Julien Favresse ◽  
Constant Gillot ◽  
Maxime Oliveira ◽  
Julie Cadrobbi ◽  
Marc Elsen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Performance ◽  
Antigen Detection ◽  
Clinical Validation ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Viral Loads ◽  
Automated Assay ◽  
Polymerase Chain ◽  
Asymptomatic Subjects

(1) Background: The detection of SARS-CoV-2 RNA in nasopharyngeal samples through real-time reverse transcription-polymerase chain reaction (RT-PCR) is considered the standard gold method for the diagnosis of SARS-CoV-2 infection. Antigen detection (AD) tests are more rapid, less laborious, and less expensive alternatives but still require clinical validation. (2) Methods: This study compared the clinical performance of five AD tests, including four rapid AD (RAD) tests (biotical, Panbio, Healgen, and Roche) and one automated AD test (VITROS). For that purpose, 118 (62.8%) symptomatic patients and 70 (37.2%) asymptomatic subjects were tested, and results were compared to RT-PCR. (3) Results: The performance of the RAD tests was modest and allowed us to identify RT-PCR positive patients with higher viral loads. For Ct values ≤25, the sensitivity ranged from 93.1% (95% CI: 83.3–98.1%) to 96.6% (95% CI: 88.1–99.6%), meaning that some samples with high viral loads were missed. Considering the Ct value proposed by the CDC for contagiousness (i.e., Ct values ≤33) sensitivities ranged from 76.2% (95% CI: 65.4–85.1%) to 88.8% (95% CI: 79.7–94.7%) while the specificity ranged from 96.3% (95% CI: 90.8–99.0%) to 99.1% (95% CI: 95.0–100%). The VITROS automated assay showed a 100% (95% CI: 95.5–100%) sensitivity for Ct values ≤33, and had a specificity of 100% (95% CI: 96.6–100%); (4) Conclusions: Compared to RAD tests, the VITROS assay fully aligned with RT-PCR for Ct values up to 33, which might allow a faster, easier and cheaper identification of SARS-CoV-2 contagious patients.

scholarly journals Considerations in Real-time Reverse Transcription Polymerase Chain Reaction (rRT-PCR) for the Detection of SARS-CoV-2 from Nasopharyngeal Swabs

2021 ◽  
pp. 68-78
Author(s):  
Priyadharshini Sekar ◽  
Godfred Antony Menezes ◽  
Pooja Shivappa ◽  
Biji Thomas George ◽  
Ashfaque Hossain
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reverse Transcription ◽  
Limit Of Detection ◽  
Disease Diagnosis ◽  
Antigen Detection ◽  
Disease Spread ◽  
Chain Reaction ◽  
The World ◽  
Polymerase Chain

Coronavirus Disease 2019 (COVID-19) was first reported in December 2019, in the City of Wuhan, China. Within the span of a few weeks, the disease had spread to other regions of China and eventually to different parts of the world. COVID 19 has affected 221 countries and territories around the world, with a total of 121,290,697 positive cases and 2,682,554 deaths as on March 17, 2021. Accurate disease diagnosis (for the SARS-Cov-2 virus and variants), coupled to patient isolation are currently critical strategies in restricting disease spread. Due to lack of time during this pandemic the diagnostics assays were not adequately validated. Infected individuals at times could potentially be missed by real-time reverse transcription polymerase chain reaction (rRT-PCR) for SARS-CoV-2 tests due to incorrect/inefficient sampling procedure, low limit of detection and epidemiology of the virus. rRT-PCR test results should be interpreted in conjunction with clinical examination and Computed Tomography (CT), particularly in suspected symptomatic individuals or those with epidemiological history of contact with known COVID-19 cases. Considering the above-mentioned constraints, the current scenario demands rapid and point-of-care tests for detection of SARS-CoV-2 in remote locations. To date, there is no reliable commercially available antigen detection kit. The infected subjects reveal low levels of antibodies against SARS-CoV-2 through the early period of infection. In addition, techniques such as, Digital RT-PCR technology and isothermal RNA amplification with electrochemical biosensors are some of the new technologies currently being developed to provide sensitive and specific SARS-Cov-2 antigen detection. The newly reported variant, SARS-CoV-2 VUI 202012/01 may not influence diagnostic outcomes as worldwide most PCR assays use two or more (including RdRp/ E/ N) reliable gene targets, besides S gene.

scholarly journals A Practical Approach for Quantitative Polymerase Chain Reaction, the Gold Standard in Microbiological Diagnosis

2021 ◽  
Author(s):  
Tímea Mosolygó ◽  
Krisztián Laczi ◽  
Gabriella Spengler ◽  
Katalin Burián
Keyword(s):  
Polymerase Chain Reaction ◽  
Undergraduate Students ◽  
Quantitative Polymerase Chain Reaction ◽  
Real Life ◽  
Medical Diagnostics ◽  
Microbiological Diagnosis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Gene Expression Studies ◽  
The Impact

From gene expression studies to identifying microbes quantitative polymerase chain reaction (qPCR) is widely used in research and medical diagnostics. In transmittable diseases like the Ebola outbreak in West Africa (2014-2016), or the present SARS-CoV2 pandemic qPCR plays a key role in the detection of infected patients. Although the technique itself is decades old with reliable approaches (eg. TaqMan essay) in the diagnosis of pathogens many people showed distrust in it during the SARS-CoV2 outbreak. This came mainly from not understanding or misunderstanding the principles of qPCR. This situation motivated us to design a simple laboratory practical class, in which students have opportunities to understand the underlying principles of qPCR and its advantages in microbiological diagnosis. Moreover, during the exercise, students can develop skills such as handling experimental assays, and the ability to solve problems, discuss their observations. Finally, this activity brings them closer to the clinical practice and they can see the impact of the science on real life. The class is addressed to undergraduate students of biological sciences.

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