Comparison of Serum Galactomannan Antigen Detection and Competitive Polymerase Chain Reaction for Diagnosing Invasive Aspergillosis

1998 ◽  
Vol 26 (6) ◽  
pp. 1407-1412 ◽  
Author(s):  
Stephane Bretagne ◽  
Jean‐Marc Costa ◽  
Emmanuelle Bart–Delabesse ◽  
Nathalie Dhedin ◽  
Claire Rieux ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Invasive Aspergillosis ◽  
Antigen Detection ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Galactomannan Antigen ◽  
Polymerase Chain

Differential display competitive polymerase chain reaction: An optimal tool for assaying gene expression

1999 ◽  
Vol 20 (2) ◽  
pp. 230 ◽  
Author(s):  
Marianne Jorgensen ◽  
Maja Bévort ◽  
Thuri S. Kledal ◽  
Brian V. Hansen ◽  
Marlene Dalgaard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Differential Display ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain

Na-K-ATPase isoform (alpha 3, alpha 2, alpha 1) abundance in rat kidney estimated by competitive RT-PCR and ouabain binding

1996 ◽  
Vol 271 (2) ◽  
pp. F253-F260 ◽  
Author(s):  
K. Lucking ◽  
J. M. Nielsen ◽  
P. A. Pedersen ◽  
P. L. Jorgensen
Keyword(s):  
Polymerase Chain Reaction ◽  
Rat Kidney ◽  
Sodium Reabsorption ◽  
Rt Pcr ◽  
Ouabain Binding ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
High Affinity ◽  
Upper Limit ◽  
Polymerase Chain

For understanding the regulation of sodium reabsorption, it is important to know whether the alpha 2- or alpha 3-isoform of Na-K-adenosinetriphosphatase (Na-K-ATPase) is expressed in mammalian kidney in addition to the predominant alpha 1 beta 1-isozyme. Here we applied competitive polymerase chain reaction (PCR) for estimation of mRNA in parenchymal zones of rat kidney for comparison to high-affinity [3H]ouabain binding. The alpha 3-isoform mRNA was demonstrated to form 0.04-0.05% of the amount of alpha 1-isoform mRNA in the cortex, medulla, and papilla of rat kidney. The alpha 2-mRNA was demonstrated in all three zones and constituted 0.03% of the amount of alpha 1-mRNA in cortex. The abundance of the alpha 1 truncated mRNA was 0.1-0.8% of that of the alpha 1-mRNA. The upper limit for expression of Na-K-ATPase isozyme with high ouabain affinity (dissociation constant, 69-141 nM) was 0.096-0.14% of the concentration of alpha 1 beta 1-Na-K-ATPase. Thus a small but well-defined pool of alpha 2- and alpha 3-isoforms constitutes < or = 0.1% of the amount of alpha 1-isoform at both the mRNA and protein level in rat kidney.

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