Skin biopsy polymerase chain reaction for rapid microbiological diagnosis in patients with purpura fulminans

2017 ◽  
Vol 177 (4) ◽  
pp. e154-e155 ◽  
Author(s):  
T. de Risi-Pugliese ◽  
A. Servy ◽  
J.-W. Decousser ◽  
B. Nebbad ◽  
M. Desroches ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Skin Biopsy ◽  
Purpura Fulminans ◽  
Microbiological Diagnosis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Inability of Polymerase Chain Reaction, Pyrosequencing, and Culture of Infected and Uninfected Site Skin Biopsy Specimens to Identify the Cause of Cellulitis

2015 ◽  
pp. civ655 ◽  
Author(s):  
Jonathan G. Crisp ◽  
Sukhjit S. Takhar ◽  
Gregory J. Moran ◽  
Anusha Krishnadasan ◽  
Scot E. Dowd ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Skin Biopsy ◽  
Chain Reaction ◽  
Biopsy Specimens ◽  
Polymerase Chain

Diagnostic Value of Polymerase Chain Reaction Analysis of Skin Biopsies in Purpura Fulminans

2013 ◽  
Vol 30 (6) ◽  
pp. e276-e277 ◽  
Author(s):  
Caroline Beau ◽  
Natalia Vlassova ◽  
Jean Sarlangue ◽  
Olivier Brissaud ◽  
Christine Léauté-Labrèze ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Purpura Fulminans ◽  
Polymerase Chain Reaction Analysis ◽  
Diagnostic Value ◽  
Chain Reaction ◽  
Skin Biopsies ◽  
Polymerase Chain

Microbiological Diagnosis of Sepsis: Polymerase Chain Reaction System Versus Blood Cultures

2015 ◽  
Vol 25 (1) ◽  
pp. 68-75 ◽  
Author(s):  
B. Suberviola ◽  
A. Marquez-Lopez ◽  
A. Castellanos-Ortega ◽  
C. Fernandez-Mazarrasa ◽  
M. Santibanez ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reaction System ◽  
Blood Cultures ◽  
Microbiological Diagnosis ◽  
Chain Reaction ◽  
Polymerase Chain Reaction System ◽  
System Versus ◽  
Polymerase Chain

scholarly journals Role of Polymerase Chain Reaction (PCR) in the Diagnosis of Paucibacillary Leprosy

2015 ◽  
Vol 4 (2) ◽  
pp. 41-48
Author(s):  
Mohammad Mahmudur Rahman ◽  
Md Akram Ullah Sikder ◽  
Lubna Khondker ◽  
Abu Hena Chowdhury ◽  
Mohammad Kamal Hossain ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Skin Biopsy ◽  
Predictive Value ◽  
Mycobacterium Leprae ◽  
Chain Reaction ◽  
Positive Skin ◽  
Major Health Problem ◽  
Bacteriological Test ◽  
Leprosy Patients ◽  
Polymerase Chain

Leprosy caused by Mycobacterium leprae, is still considered a major health problem in many developing countries. The routine bacteriological test on the demonstration of acid-fast bacilli in skin smears is not sufficiently sensitive or specific in the paucibacillary leprosy patients. To detect DNA of Mycobacterium leprae by Polymerase Chain Reaction (PCR) from skin biopsy specimen as a means of diagnosis of paucibacillary leprosy. It was a cross-sectional study of clinically diagnosed 33 cases of Paucibacillary leprosy patients. Laboratory works were performed in the Department of Microbiology and Immunology and Department of pathology; Bangabandhu Sheikh Mujib Medical University (BSMMU) and Leprosy Control Institute and Hospital, Mohakhali, Dhaka from November 2013 to June 2015. It was observed that the mean age of patients was found 30.9±13.7 years, 19(57.6%) patients had two to five skin lesions, all (100%) patients had tuberculoid type of leprosy, all (100%) patients had loss of sensation and 18(54.5%) patients had nerve thickening. Regarding slit skin smear test, it was observed that all (100%) patients were slit skin smear negative for leprosy bacilli, 8(24.2%) patients had positive skin biopsy for histopathology and 15(45.5%) patients had positive skin biopsy for PCR. The validity of PCR for identification of paucibacillary leprosy correlated with sensitivity 100%, specificity 72%, accuracy 78.8%, positive predictive value 53.3% and negative predictive value 100%. Polymerase chain reaction (PCR) has definite value in the diagnosis of paucibacillary leprosy and can be regarded as a sensitive and specific diagnostic modality. CBMJ 2015 July: Vol. 04 No. 02 P: 41-48

scholarly journals A Practical Approach for Quantitative Polymerase Chain Reaction, the Gold Standard in Microbiological Diagnosis

2021 ◽  
Author(s):  
Tímea Mosolygó ◽  
Krisztián Laczi ◽  
Gabriella Spengler ◽  
Katalin Burián
Keyword(s):  
Polymerase Chain Reaction ◽  
Undergraduate Students ◽  
Quantitative Polymerase Chain Reaction ◽  
Real Life ◽  
Medical Diagnostics ◽  
Microbiological Diagnosis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Gene Expression Studies ◽  
The Impact

From gene expression studies to identifying microbes quantitative polymerase chain reaction (qPCR) is widely used in research and medical diagnostics. In transmittable diseases like the Ebola outbreak in West Africa (2014-2016), or the present SARS-CoV2 pandemic qPCR plays a key role in the detection of infected patients. Although the technique itself is decades old with reliable approaches (eg. TaqMan essay) in the diagnosis of pathogens many people showed distrust in it during the SARS-CoV2 outbreak. This came mainly from not understanding or misunderstanding the principles of qPCR. This situation motivated us to design a simple laboratory practical class, in which students have opportunities to understand the underlying principles of qPCR and its advantages in microbiological diagnosis. Moreover, during the exercise, students can develop skills such as handling experimental assays, and the ability to solve problems, discuss their observations. Finally, this activity brings them closer to the clinical practice and they can see the impact of the science on real life. The class is addressed to undergraduate students of biological sciences.

scholarly journals Microbiological diagnosis of pulmonary tuberculosis in children by oral swab polymerase chain reaction

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mark P. Nicol ◽  
Rachel C. Wood ◽  
Lesley Workman ◽  
Margaretha Prins ◽  
Cynthia Whitman ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pulmonary Tuberculosis ◽  
Microbiological Diagnosis ◽  
Chain Reaction ◽  
Oral Swab ◽  
Polymerase Chain ◽  
Tuberculosis In Children
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