Isolation of Ureaplasma urealyticum and Mycoplasma hominis From Public Toilet Bowls

1999 ◽  
Vol 20 (01) ◽  
pp. 66-68 ◽  
Author(s):  
Israel Potasman ◽  
Amnon Oren ◽  
Isaac Srugo
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Antigen Detection ◽  
Chain Reaction ◽  
Survival Assay ◽  
Polymerase Chain

Abstract The presence of Ureaplasma urealyticum, Mycoplasma hominis, and Chlamydia trachomatis was explored in 50 public restroom toilet bowls. We used culture, antigen detection, polymerase chain reaction, and survival assay. Five bowls (10%) were contaminated with at least one organism. U urealyticum was found in four bowls, M hominis in three, and C trachomatis in one. U urealyticum survived on the toilet rim for up to 2 hours.

scholarly journals CLINICAL AND IMMUNOLOGICAL CHANGES OF NON-GONOCOCCAL URETHRITIS

2020 ◽  
Vol 22 (1) ◽  
pp. 40-44
Author(s):  
Gadoev Maruf ◽  
◽  
Bakhromuddin Saidzoda ◽  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Control Group ◽  
Direct Immunofluorescence ◽  
Chain Reaction ◽  
Immunological Disorders ◽  
Gonococcal Urethritis ◽  
Polymerase Chain

Objective: To study the clinical features and the state of general immunological reactivity in non-gonococcal urethritis (NGU) in men. Methods: Examined 100 men between the ages of 20 and 48 years: 75 patients of NGU (main group) and 25 healthy (control group). The average age of the patients was 26.7±1.7 years, and the male of control group was 27.9±1.7 years. Clinical, microscopic, immunological research methods were used, including direct immunofluorescence (DIF), polymerase chain reaction (PCR). Results: Ureaplasma urealyticum was found in 37 (49.3%) patients, 33 (44%) had Chlamydia trachomatis, 23 (30.7%) had Mycoplasma genitalium, 16 (21.3%) had Trichomonas vaginalis. In 24 (32%) of NGU patients had a mixed infection: in 14 (18.7%) had a combination of two STIs and in 10 (13, 3%) had three infections. In 51 (68%) of patients the process passed in the form of monoinfection. Various complaints (dysuric disorders, pain, discomfort and agglutination of the labium urethra) were presented by 51 (68%) of sick patients. The excretions from the urethra were marked in 46 (61.3%) of patients, reproductive disorders are 3 times less common. Immunological disorders were manifested by a decrease in CD4 and CD8 lymphocytes, PHA, PN and IL-10, increase – IgM, IgG, CIC, TNFα, IL-1β. Conclusions: The most common cause of NGU is Ureaplasma urealyticum and Chlamydia trachomatis. In most cases NGU proceeds in the form of monoinfection. Subjective and objective symptoms occur in 64% and 59% of patients, respectively. Immunological disorders were detected in 71% of patients. Keywords: Non-gonogococcal urethritis, direct immunofluorescence, immunoenzyme method, polymerase chain reaction

scholarly journals Detection of mycoplasmas in urethral swabs from HIV-1 infected patients and control individuals using culture techniques and polymerase chain reaction

1998 ◽  
Vol 40 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Regina Ayr Florio da CUNHA ◽  
Kioko TAKEI ◽  
Adelaide José VAZ ◽  
Caio ROSENTHAL
Keyword(s):  
Polymerase Chain Reaction ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Species ◽  
Control Group ◽  
Chain Reaction ◽  
Culture Techniques ◽  
Significant Difference ◽  
Polymerase Chain ◽  
Hiv 1

The objective of the present study was to determine the prevalence of certain mycoplasma species, i.e., Mycoplasma hominis, Ureaplasma urealyticum and Mycoplasma penetrans, in urethral swabs from HIV-1 infected patients compared to swabs from a control group. Mycoplasmas were detected by routine culture techniques and by the Polymerase Chain Reaction (PCR) technique, using 16SrRNA generic primers of conserved region and Mycoplasma penetrans specific primers. The positivity rates obtained with the two methods were comparable. Nevertheless, PCR was more sensitive, while the culture techniques allowed the quantification of the isolates. The results showed no significant difference (p < 0.05) in positivity rates between the methods used for mycoplasma detection.

Detection of the sexually transmissible genital mycoplasmas by polymerase chain reaction in women

Sexual Health ◽  
2011 ◽  
Vol 8 (3) ◽  
pp. 445 ◽  
Author(s):  
Vessela V. Ouzounova-Raykova ◽  
Rumyana Markovska ◽  
Gergana Mizgova ◽  
Ivan G. Mitov
Keyword(s):  
Polymerase Chain Reaction ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Chain Reaction ◽  
Clinical Disorders ◽  
Polymerase Chain ◽  
Asymptomatic Women ◽  
Genital Mycoplasmas

Background The role of Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium in the development of clinical disorders is still unclear. The aim of this study was to determine their prevalence in symptomatic and asymptomatic women. Methods: A total of 348 women were examined by applying polymerase chain reaction (PCR) methods. Results: The registered prevalence was as follows: U. urealyticum 14.66%; M. hominis 3.16%; and M. genitalium 0.29%. Co-infection was established in 11 swabs. Conclusions: This is the first study in Bulgaria for the detection of mycoplasmas by PCR. Our results demonstrate similar or lower values in comparison with other researchers and further investigations are needed.

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