scholarly journals The effect of heat on amino acids for growing pigs

1994 ◽  
Vol 72 (2) ◽  
pp. 257-275 ◽  
Author(s):  
R. J. Van Barneveld ◽  
E. S. Batterham ◽  
B. W. Norton
Keyword(s):  
Heat Treatment ◽  
Dietary Protein ◽  
Growing Pigs ◽  
Slope Ratio ◽  
Ileal Digestibility ◽  
Protein Concentrates ◽  
Dietary Protein Level ◽  
Heat Treated ◽  
Field Peas ◽  
Forced Air

The effect of heat on the availability of lysine in field peas (Pisum sativum cultivar Dundale) was determined using the slope–ratio assay with growing pigs. The field peas were heated to 110°, 135°, 150°, or 165° for 15 min using a forced-air dehydrator. Lysine availability was significantly depressed (P < 0.05) with the application of heat, even at mild temperatures of 110°. Lysine availability values of 0.96, 0.71, 0.77, 0.56, and 0.47 were determined for the raw peas and peas heated to 110°, 135°, 150° or 165° respectively. The effect of dietary protein level on the ileal digestibility of lysine in raw field peas was determined in a second experiment to estimate true ileal digestibility. Five diets were formulated to contain 85, 105, 125, 145 and 165 g protein/kg. Increasing dietary protein from 85 to 105 g/kg resulted in a significant increase in the estimate of apparent ileal digestibility from 0.84 to 0.93. Comparisons were then able to be drawn between the ileal digestibility, availability and utilization of lysine from raw and heat-treated field peas. The results indicate that unlike ileal digestibility values, estimates of lysine availability are sensitive to heat treatment and are a close reflection of lysine utilization in heated protein concentrates. Thus, lysine availability values would be more suitable for use in diet formulations than ileal digestibility estimates when dealing with heat-processed protein concentrates.

scholarly journals The effect of heat on amino acids for growing pigs

1994 ◽  
Vol 72 (2) ◽  
pp. 243-256 ◽  
Author(s):  
R. J. Van Barneveld ◽  
E. S. Batterham ◽  
B. W. Norton
Keyword(s):  
Amino Acids ◽  
Crude Protein ◽  
Growing Pigs ◽  
Quadratic Effect ◽  
Ileal Digestibility ◽  
Protein Deposition ◽  
Growing Pig ◽  
Field Peas ◽  
Forced Air ◽  
Growth Experiments

Two growth experiments were conducted to determine the effect of heat on the utilization of ileal-digestible lysine from field peas (Pisum sativum cultivar Dundale) fed to growing pigs. Five lysine-deficient diets (0.36 g ileal-digestible lysine/MJ digestible energy (DE)) were formulated using raw field peas, and field peas heated to either 110°, 135°, 150°, or 165° for 15 min respectively in a forced-air dehydrator. Additional diets were formulated with supplements of free lysine to verify that lysine was limiting in the diets containing the raw peas, and peas heated to 150° or 165°. The growth performance and retention of ileal-digestible lysine by pigs given the diets was determined over the 20–45 kg growth phase. Heat had a significant quadratic effect (P < 0.01) on growth rate, with responses declining from 543 g/d with pigs given the raw peas, to 407 g/d for those given the peas heated to 165°. Similarly, crude protein deposition declined in a quadratic manner (P < 0.001) from 76 to 36 g/d for pigs fed on raw peas and peas heated to 165° respectively. Retention of ileal-digestible lysine was 0.85 in the pigs given the raw field peas and declined in a quadratic manner (P < 0.001) with the application of heat to 0.48 in those pigs given the peas heated to 165°. Pigs fed on field peas heated to 165° had increased (P < 0.05) liver weights. The results indicate that heat applied to protein concentrates, even at mild temperatures, renders lysine in a form that is apparently absorbed but inefficiently utilized by the growing pig. Consequently, ileal digestibility values for lysine in heat-processed meals are unsuitable for diet formulations.

scholarly journals Availability of lysine in vegetable protein concentrates as determined by the slope-ratio assay with growing pigs and rats and by chemical techniques

1984 ◽  
Vol 51 (1) ◽  
pp. 85-99 ◽  
Author(s):  
E. S. Batterham ◽  
R. D. Murison ◽  
L. M. Andersen
Keyword(s):  
Cottonseed Meal ◽  
Vegetable Protein ◽  
Soya Bean ◽  
Growing Pigs ◽  
Slope Ratio ◽  
Protein Concentrates ◽  
Field Peas ◽  
Available Lysine ◽  
Bean Meal ◽  
Soya Bean Meal

1. The availability of lysine in nine vegetable-protein concentrates was assessed using the slope-ratio assay for growing pigs and rats. Diets were equalized for crude fibre using solka floc to minimize any possible effects of variation in fibre content on availability estimates.2. The availability of lysine in the nine proteins for pigs, using food conversion efficiency (FCE) on a carcass basis as the criterion of response were (proportion of total): cottonseed meal 0.39, lupin (Lupinus angustifolius) seed meal no. 10.37, no. 20.65, no. 30.54, no. 40.54, field peas (Pisum sativum) 0.93, peanut (groundnut) meal 0.57, soya-bean meal no. 10.98, no. 2 0.89.3. Estimates of available lysine for rats as assessed by the slope-ratio assay using FCE on a carcass basis were in close agreement with the pig estimates for cottonseed meal (0.35) and soya-bean meal no. 1(0.91) and no. 2(0.89), higher for lupin-seed meals (range 0.70–0.94 with a mean of 0.81) and peanut meal (0.76) and lower for field peas (0.76).4. The differences in available lysine were not detected by the chemical Silcock available-lysine test (Roach et al. 1967) or by the direct 1-fluoro-2,4-dinitrobenzene procedure (Carpenter, 1960).

scholarly journals Protein quality in experimental diets and amino acid digestibility

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 28-29
Author(s):  
Chan Sol Park ◽  
Olayiwola Adeola
Keyword(s):  
Amino Acid ◽  
Dietary Protein ◽  
Protein Quality ◽  
Sole Source ◽  
Growing Pigs ◽  
Ileal Digestibility ◽  
Distillers Dried Grains ◽  
Low Protein ◽  
Dietary Casein ◽  
Dietary Treatments

Abstract Digestibility of AA in feed ingredients for pigs has been generally determined by feeding semipurified diets containing test ingredients as a sole source of nitrogen. However, malnutrition caused by feeding semi-purified diets may affect the digestibility of AA. Therefore, the hypothesis of this study was that the standardized ileal digestibility (SID) of AA in distillers’ dried grains with solubles (DDGS) are not affected by the addition of casein in experimental diets. In Exp. 1, 20 growing pigs were allotted to dietary treatments including 2 diets containing either 480 g/kg DDGS or 308 g/kg DDGS and 60 g/kg casein. The SID of Lys and Phe for DDGS in the diet containing casein were greater (P < 0.05) than those without casein. Exp. 2 was conducted with 20 growing pigs assigned to dietary treatments containing 0, 55, 110, or 165 g/kg casein while the concentration of DDGS decreased at 466.8, 311.2, 155.6, or 0 g/kg. The SID of most indispensable AA in DDGS linearly (P < 0.05) decreased with increasing concentration of casein. Experiment 3 was conducted to verify that the results of Exp. 1 were affected by the addition of casein or by the concentration of DDGS or both. Twenty growing pigs were assigned to dietary treatments prepared as a 2 × 2 factorial arrangement with the concentration of DDGS at 466.8 or 155.6 g/kg and casein at 0 or 110 g/kg. The SID of most indispensable AA in DDGS at 466.8 g/kg were greater (P < 0.01) than in DDGS at 155.6 g/kg regardless of dietary casein. Overall, the addition of 60 g/kg casein to experimental diets may increase the SID of AA in low-protein quality ingredients but the addition of higher concentration of casein and low concentration of dietary protein from test ingredients may decrease the SID of AA.

Utilization of ileal digestible amino acids by growing pigs: isoleucine

1994 ◽  
Vol 71 (4) ◽  
pp. 531-541 ◽  
Author(s):  
E. S. Batterham ◽  
L. M. Andersen
Keyword(s):  
Amino Acids ◽  
Cottonseed Meal ◽  
Soya Bean ◽  
Growing Pigs ◽  
Seed Meal ◽  
Ileal Digestibility ◽  
Protein Concentrates ◽  
Lupin Seed ◽  
Bean Meal ◽  
Soya Bean Meal

Two experiments were conducted to determine the utilization of ileal digestible isoleucine by growing pigs. In the first, the apparent ileal digestibility of amino acids in cottonseed meal, lupin-seed meal and soya-bean meal was determined in pigs fitted with‘T‘-shaped cannulas. In the second, three isoleucine-deficient diets were formulated to 0.23 g ileal digestible isoleucine/MJ digestible energy (DE) with the three protein concentrates contributing the only source of isoleucine in sucrose-based diets. An additional three diets were formulated with supplements of isoleucine to confirm that isoleucine was limiting in the first three diets. The growth performance and retention of isoleucine by pigs given the six diets over the 20–45 kg growth phase were then determined. The apparent ileal digestibility of isoleucine in the three protein concentrates (proportion of total) was: cottonseed meal 0.68, lupin-seed meal 0.86, soya-bean meal 0.86. There were no significant differences (P > 0.05) in growth rates (g/d) and crude protein deposition rates (g/d) of the pigs given the three diets formulated to 0.23 g ileal digestible isoleucine/MJ DE: cottonseed meal 590, 84; lupin-seed meal 613, 87; soya-bean meal 594, 91 (SEM 13.0, 2.9) respectively. The response of pigs to the addition of isoleucine confirmed that isoleucine was limiting in these diets. The proportion of ileal digestible isoleucine retained by pigs given the cottonseed meal (0.65) was slightly lower than that retained by pigs given soya-bean meal (0.73; P < 0.05). These results indicate that values for the ileal digestibility of isoleucine in protein concentrates more closely reflect the proportion of isoleucine that can be utilized by the pig than occurs for other amino acids such as lysine, threonine and methionine.

scholarly journals The effect of dietary crude protein content on apparent and true ileal nitrogen and amino acid digestibilities

1994 ◽  
Vol 72 (1) ◽  
pp. 59-68 ◽  
Author(s):  
A. Donkoh ◽  
P. J. Moughan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Content ◽  
Dietary Protein ◽  
Crude Protein ◽  
Apparent Digestibility ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Ileal Digestibility ◽  
Dietary Protein Level

The effect of dietary protein content (25, 60, 95, 130, 165 and 200 g crude protein (N × 6.25)/kg diet) on the apparent and true ileal digestibilities of N and amino acids in meat-and-bone meal given to the growing rat was investigated. Semi-synthetic diets in which meat-and-bone meal was the sole protein source were given to 180 g body-weight rats for 14 d. On the fourteenth day the rats were fed and then killed 4 h after the start of feeding and digesta were sampled from the terminal 200 mm ileum. Endogenous amino acid excretion was determined for eighteen rats given an enzymically hydrolysed casein (EHC)-based diet and with subsequent treatment of the digesta using ultrafiltration. The EHC-fed rats were killed 3 h after the start of feeding and digesta were collected from the terminal 200 mm ileum. True ileal digestibility values determined with reference to Cr as a marker were higher than the corresponding apparent estimates. Apparent digestibility values of N and amino acids increased significantly (P < 0.001) with increasing dietary protein level; however, dietary protein content had no significant (P > 0.05) effect on the true ileal digestibilities of N and amino acids. The mean apparent ileal digestibility of N in meat-and-bone meal ranged from 65.6 to 75.3%. The corresponding range for the true ileal digestibility of N was 76.9 to 78.2%. True ileal digestibility, unlike apparent digestibility, appears to be independent of dietary protein level and may allow feed ingredients to be compared accurately even if they are ingested in different quantities.

scholarly journals Ileal digestibility of dietary protein in the growing pig and adult human

2009 ◽  
Vol 102 (12) ◽  
pp. 1752-1759 ◽  
Author(s):  
Amélie Deglaire ◽  
Cécile Bos ◽  
Daniel Tomé ◽  
Paul J. Moughan
Keyword(s):  
Body Weight ◽  
Dietary Protein ◽  
Human Subjects ◽  
Protein Digestibility ◽  
Sole Source ◽  
Growing Pigs ◽  
Ileal Digestibility ◽  
Adult Human ◽  
Growing Pig ◽  
Hydrolysed Casein

The suitability of the pig as an animal model for predicting protein digestibility in man was evaluated. Healthy adult human subjects (mean body weight 67 kg;n11) and growing pigs (mean body weight 40 kg;n15) were fed semi-synthetic mixed meals containing, as a sole source of N, casein (C), hydrolysed casein (HC) or rapeseed isolate (R). There was no prior adaptation to the test meal. Ileal digesta were sampled through a naso-ileal tube (human subjects) or a post-valve T-caecum cannula (pigs) after ingestion of a bolus meal. The protein sources were15N-labelled. Amino acid (AA) digestibilities were not determined for R. Ileal apparent N digestibility was markedly lower (14–16 %;P < 0·001) in human subjects than in pigs (C, HC, R). Similarly, most apparent ileal AA digestibilities were lower (8 % on average;P < 0·05) in human subjects (C, HC). Ileal true N digestibility was slightly lower (3–5 %;P < 0·001) in human subjects than in pigs (C, HC, R) and most true ileal AA digestibilities were similar (P>0·05) between the species (C, HC). Exceptions were for phenylalanine, tyrosine, lysine, histidine and aspartic acid for which digestibilities were lower (3 % on average;P < 0·001) in human subjects. A similar ranking of the diets was observed for true ileal N digestibility between species. The inter-species correlation for true ileal digestibility was high for N (r0·98 over 3 × 2 data;P = 0·11) and AA (r0·87 over 26 × 2 data;P < 0·0001). Overall, this supports the use of the pig as a model for predicting differences among dietary protein digestibility, especially regarding true ileal N digestibility, in man.

scholarly journals Nitrogen balance response to varying levels of dietary protein in slow-growing Windsnyer pigs

2021 ◽  
Vol 50 (5) ◽  
Author(s):  
V.A. Hlatini ◽  
C.N. Ncobela ◽  
M. Chimonyo
Keyword(s):  
Linear Relationship ◽  
Total Nitrogen ◽  
Nitrogen Balance ◽  
Dietary Protein ◽  
Protein Level ◽  
Growing Pigs ◽  
Nitrogen Utilization ◽  
Dietary Protein Level ◽  
Quadratic Relationship ◽  
Slow Growing

Protein requirements for slow-growing pigs have not been defined. The study was designed to determine the relationship between the level of dietary protein and nitrogen balance in slow-growing Windsnyer pigs. Thirty Windsnyer pigs with an initial bodyweight of 34.7 (SD = 0.85) kg were randomly assigned to six experimental diets containing 193, 174, 154, 135, 117, and 97 g/kg of crude protein (CP). Pigs were penned individually. There were five pigs in each diet. The faecal nitrogen output (NFO) showed a decreasing quadratic relationship with protein level. A linear relationship between protein level and urinary nitrogen excretion was observed (P =0.0001). The total nitrogen excreted showed a significant quadratic relationship to decreasing levels of CP in the diets (P =0.0001). The apparent digestibility of nitrogen had a linear relationship with dietary CP level (P =0.0005). A quadratic relationship between dietary protein level and N utilization was observed (P =0.0001). The optimum protein level for total nitrogen excreted, nitrogen utilization and biological value of feed protein were attained at 135 g/kg protein level. The efficiency of nitrogen utilization supports the low protein diet. The present results suggest reducing the dietary protein level for Windsnyer pigs to decrease amount of excreted N that is deposited into the environment.Protein requirements for slow-growing pigs have not been defined. The study was designed to determine the relationship between the level of dietary protein and nitrogen balance in slow-growing Windsnyer pigs. Thirty Windsnyer pigs with an initial bodyweight of 34.7 (SD = 0.85) kg were randomly assigned to six experimental diets containing 193, 174, 154, 135, 117, and 97 g/kg of crude protein (CP). Pigs were penned individually. There were five pigs in each diet. The faecal nitrogen output (NFO) showed a decreasing quadratic relationship with protein level. A linear relationship between protein level and urinary nitrogen excretion was observed (P =0.0001). The total nitrogen excreted showed a significant quadratic relationship to decreasing levels of CP in the diets (P =0.0001). The apparent digestibility of nitrogen had a linear relationship with dietary CP level (P =0.0005). A quadratic relationship between dietary protein level and N utilization was served (P =0.0001). The optimum protein level for total nitrogen excreted, nitrogen utilization and biological value of feed protein were attained at 135 g/kg protein level. The efficiency of nitrogen utilization supports the low protein diet. The present results suggest reducing the dietary protein level for Windsnyer pigs to decrease amount of excreted N that is deposited into the ironment. Keywords: apparent nitrogen digestibility, ideal protein level, nitrogen retention, excreted nitrogen, nitrogen utilization

scholarly journals Quality of Stored `Marsh' and `Ruby Red' Grapefruit after High-temperature, Forced-air Treatment

HortScience ◽  
1991 ◽  
Vol 26 (9) ◽  
pp. 1188-1191 ◽  
Author(s):  
W.R. Miller ◽  
R.E. McDonald
Keyword(s):  
Heat Treatment ◽  
High Temperature ◽  
Storage Period ◽  
Fruit Fly ◽  
Citrus Paradisi ◽  
Caribbean Fruit Fly ◽  
Heat Treated ◽  
Fruit Decay ◽  
Forced Air ◽  
Subsequent Storage

`Marsh' and `Ruby Red' grapefruit (Citrus paradisi Macf.) tolerated a high-temperature, forced-air, vapor heat treatment of 43.5C for 260 minutes, a treatment applied for security against the Caribbean fruit fly [Anastrepha suspensa (Loew)]. Fruit did not develop symptoms of quality deterioration during subsequent storage. With `Marsh' fruit, 99% and 96% were sound, whereas with `Ruby Red' 98% and 94% were sound after storage at 10C for 28 days or 10C for 28 days plus 7 days at 21C, respectively. Differences in means for percentage of sound fruit were not significant for cultivar or vapor heat treatment. After the final storage period, there was significantly more (2.4-fold, P ≤ 0.05) aging observed on `Ruby Red' fruit than on `Marsh', averaged over all treatments. Vapor heat did not affect aging of `Ruby Red' but increased aging of `Marsh' fruit. Decay was reduced to ≈ 22.0% in vapor heat-treated fruit from 5.0% for nontreated fruit. The efficacy of thiabendazole to control stem end rot was increased on vapor heat-treated fruit compared with nontreated fruit. After the final inspection, the appearance of `Marsh' fruit was fresher (index 2.0) than that of `Ruby Red' fruit (index 2.3), but the appearance of vapor heat-treated and nontreated fruit was similar. Peel color of `Ruby Red' fruit was not affected by the vapor heat treatment, but, after 4 weeks at 10C plus 1 week at 21C, `Marsh' fruit that were not treated were greener than those treated with vapor heat. The vapor heat treatment tested is a potentially viable quarantine procedure for Florida grapefruit that can be applied without adversely affecting fruit quality during normal storage.

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