scholarly journals The effect of condensed tannins on the site of digestion of amino acids and other nutrients in sheep fed on Lotus corniculatus L

1987 ◽  
Vol 57 (1) ◽  
pp. 115-126 ◽  
Author(s):  
G. C. Waghorn ◽  
M. J. Ulyatt ◽  
A. John ◽  
M. T. Fisher
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Condensed Tannins ◽  
Dry Matter ◽  
Condensed Tannin ◽  
Lotus Corniculatus ◽  
Essential Amino Acids ◽  
Control Group ◽  
Apparent Digestibility ◽  
Tannin Concentration

1. Sheep were used to evaluate the nutritional consequences of a low condensed-tannin concentration (22 g/kg dry matter (DM)) in lotus (Lotus corniculatus L.) (control group) compared with lotus given to sheep receiving intraruminal polyethylene glycol (PEG) infusion (PEG group). PEG selectively binds to tannins and prevents tannins from binding proteins.2. DM intakes (1430 (SE 28) g/d) and digestibility of energy (663 (SE 4.5) kJ/MJ intake) were similar for both groups but the apparent digestion of nitrogen was lower in the control sheep (0.70) than in the PEG sheep (0.78; P < 0.001).3. The proportion of N apparently digested before the abomasum (i.e. in the rumen) was lower (P < 0.05) in control sheep (0.12) than in PEG sheep (0.21; P < 0.05). Rumen ammonia concentrations were lower (P < 0.001) in control sheep than in PEG sheep. The proportion of neutral-detergent fibre (NDF) digested in the rumen was similar for both groups (0.48 (SE 0.012)) but less energy was digested in the rumen of the control (0.42) than of the PEG sheep (0.47; P < 0.05).4. The flux of essential amino acids (EAA) through the abomasum of control sheep was 50% greater than that in PEG sheep; flux of non-essential amino acids (NEAA) was 14% higher in control than in PEG sheep. Apparent digestibility of EAA in the small intestine was similar for both treatments (0.67), but NEAA were less well digested in the control (0.55) than in the PEG sheep (0.69).5. The presence of tannins in the control group increased net apparent absorption of threonine (57%), valine (89%), isoleucine (94%), leucine (30%), tyrosine (41%), phenylalanine (93%), histidine (90%) and lysine (59%), and reduced NEAA absorption by 10%, compared with PEG sheep.

Effect of condensed tannins in Lotus corniculatus upon the digestion of methionine and cysteine in the small intestine of sheep

1996 ◽  
Vol 127 (3) ◽  
pp. 413-421 ◽  
Author(s):  
Y. Wang ◽  
G. C. Waghorn ◽  
W. C. McNabb ◽  
T. N. Barry ◽  
M. J. Hedley ◽  
...  
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Small Intestine ◽  
Polyethylene Glycol ◽  
Liquid Phase ◽  
Condensed Tannins ◽  
Dry Matter ◽  
Apparent Digestibility ◽  
True Digestibility ◽  
Site Of Absorption

SUMMARYAn experiment was conducted at Palmerston North, New Zealand, to determine the effect of condensed tannins (CT) on the true and apparent digestion of methionine and cysteine in the small intestine (SI) of sheep fed fresh Lotus comkulatus. The lotus contained c. 30 g total CT/kg dry matter (DM) and was fed hourly to sheep in metabolism crates. Four sheep were prepared with rumen and abomasal cannulae which enabled the indigestible liquid phase marker, chromium ethylene diamine tetra-acetic acid (Cr-EDTA), to be infused into the rumen to estimate digesta flow. True digestibility of plant methionine and cysteine in the SI and their site of absorption in the SI were determined from 35S-labelled L. corniculatus homogenate continuously infused into the abomasum. After 9 h infusion of the 35S-labelled lotus homogenate, the sheep were slaughtered and digesta samples were taken at intervals along the small and large intestines. The effect of CT was determined by comparing two control sheep (CT-acting) with two sheep given a continuous intraruminal infusion of polyethylene glycol (PEG, MW 3500) to bind and inactivate the CT.The CT reduced the true digestibility of plant methionine (0·72 v. 0·88) and cysteine (0·65 v. 0·81) in the SI relative to sheep receiving PEG. Condensed tannins also appeared to alter the site of digestion of both [35S]methionine and [35S]cysteine in the SI, and increased the flux of both amino acids in the mid and latter thirds of the SI. CT did not affect the apparent digestibility of total methionine (0·82 v. 0·84) in the SI but reduced the apparent digestibility of total cysteine from 0·77 to 0·66. In control sheep CT increased the abomasal flux (as a proportion of eaten) of total digesta methionine (0·88 v. 0·76) and total digesta cysteine (0·74 v. 0·62). The apparent absorption of total methionine (plant + microbial + endogenous) was increased by the action of CT (0·72 v. 0·63 g/g eaten) but was similar for total cysteine (0·49 v. 0·48 g/g eaten) in both groups. It was concluded that CT reduced the true digestibility of plant methionine and cysteine in the SI. However, it was calculated that the action of CT actually increased the total amounts (g/g eaten) of plant methionine and cysteine absorbed from the SI, due to its effect in increasing abomasal flux.

scholarly journals Apparent digestibility of wheat and coproducts in extruded diets for the Nile tilapia, Oreochromis niloticus

2017 ◽  
Vol 18 (3) ◽  
pp. 479-491
Author(s):  
Luiz Vítor Oliveira VIDAL ◽  
Tadeu Orlandi XAVIER ◽  
Lorena Batista de MOURA ◽  
Mariana MICHELATO ◽  
Elias Nunes MARTINS ◽  
...  
Keyword(s):  
Amino Acids ◽  
Winter Wheat ◽  
Wheat Bran ◽  
Nile Tilapia ◽  
Crude Protein ◽  
Wheat Germ ◽  
Dry Matter ◽  
Essential Amino Acids ◽  
Apparent Digestibility ◽  
Gross Energy

SUMMARY In the present study, we investigated the digestibility of wheat and its coproducts in extruded diets for juvenile Nile tilapia. Specifically, we evaluated the apparent digestibility coefficients (ADCs) of dry matter (ADCDM), gross energy (ADCGE), crude protein (ADCCP), essential amino acids (EAAs), and non-essential amino acids (NEAAs) in winter wheat, spring wheat, wheat middling, wheat bran, and wheat germ in a diet fed Nile tilapia. A reference diet and test diets were used, with 5.0g/kg chromic oxide (Cr2O3) as the external indicator. The ADCDM, ADCCP, and ADCGE were higher (P< 0.05) in winter wheat, spring wheat, and wheat germ than for wheat middling and wheat bran. The ADCs of all amino acids (AAs) were >80%, except for threonine in wheat middling and wheat bran, exceeding 90% for winter wheat and wheat germ. The values of all investigated ingredients were high for ADCCP, and the ADCs of EAAs and NEAAs. A strong negative correlation was detected for crude fiber and ash with the ADCs of dry matter, gross energy, and crude protein. In general, wheat ingredients have good digestibility of protein and amino acids, but high fiber content reduces CDAMS and CDAEB in extruded diets for Nile tilapia.

scholarly journals Digestion, absorption and utilization of single-cell protein by the preruminant calf

1985 ◽  
Vol 54 (1) ◽  
pp. 219-244 ◽  
Author(s):  
Cynthia A. Sedgman ◽  
J. H. B. Roy ◽  
Joanne Thomas ◽  
I. J. F. Stobo ◽  
P. Ganderton
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Amino Acid ◽  
Milk Protein ◽  
Alimentary Tract ◽  
Latin Square ◽  
Essential Amino Acids ◽  
Apparent Digestibility ◽  
Bacterial Protein ◽  
True Digestibility

1.Two experiments of Latin square design were made, each with four Friesian bull calves fitted with re-entrant duodenal and ileal cannulas at 4–10 d of age. The calves were used to studythe effect of giving milk-substitutes containing 0, 300, 500 and 700 g bacterial protein (Pruteen)/kg total protein on apparent digestibility of nitrogen fractions and amino acids and true digestibility of 3H-labelled milk protein and 35S-labelled bacterial protein in the small intestine. A third experiment of Latin square design with four intact Friesian calves was used to measure apparent digestibility of nutrients throughout the alimentary tract and retention of N, calcium and phosphorus.2.At the duodenum, volume of outflow, its pH, and outflow of total-N (TN), protein-N (PN) and non-protein-N (NPN) decreased with time after feeding. At the ileum, volume of outflow and TN outflow were unaffected by time after feeding but PN outflow decreased; NPN outflow at the ileum increased to a maximum 6 h after feeding and then declined.3.Increased inclusion of Pruteen did not affect the volume of outflow at the duodenum or ileum, but duodenal PN outflow increased. At the ileum, pH values were lower and TN, PN and NPN outflows were higher with increasing concentration of Pruteen in the diet. Apparent digestibility in the small intestine tended to decrease with greater amounts of Pruteen, but was only significant for NPN. Apparent digestibility from mouth to ileum significantly decreased for TN and PN as Pruteen inclusion increased.4.Amino acid concentration in duodenal outflow, with the exception of that of arginine, reflected intake. The total amount of each amino acid in ileal outflow increased and the apparent digestibility of most amino acids decreased with greater amounts of Pruteen in the diet. Apparent digestibility of nucleic acid-N from Pruteen was very high.5.True digestibility in the small intestine and between mouth and ileum of 3H-labelled milk protein was high and did not differ between dietary treatments. True digestibility of 36S-labelled Pruteen was low for the milk-protein diet and tended to increase linearly as more dietary Pruteen was included.6.Dry matter concentration in faeces and a high apparent digestibility throughout the whole alimentary tract of carbohydrates did not differ between treatments. Apparent digestibility of dry matter, organic matter, crude protein and fat, apparent absorption of Ca, P and ash throughout the tract, retention of N, Ca and P and biological value of the protein decreased with inclusion rates greater than 300 g Pruteen/kg total dietary protein. The amount of N apparently absorbed in the large intestine was estimated as 0.9 g/d.7.Comparison of intake of apparently absorbed essential amino acids with requirement suggests that histidine is likely to be the limiting amino acid, assuming that arginine is synthesized in the body.8.Efficiencies of utilization of protein for tissue synthesis and to cover obligatory loss are estimated as 0.80, 0.75, 0.66 and 0.47 for diets containing 0, 300, 500 and 700 g Pruteen/kg total protein in the diet. Efficiencies of utilization of individual essential amino acids were also estimated.

scholarly journals Addition of Tannin in Lamb Diets after Weaning: Impact on Performance and Hematological and Biochemical Variables

2019 ◽  
Vol 47 (1) ◽  
Author(s):  
Eduardo Lucianer ◽  
Aleksandro Schafer da Silva ◽  
Chrystian Jassana Cazarotto ◽  
Davi Fernando Alba ◽  
Luiz Gustavo Griss ◽  
...  
Keyword(s):  
Amino Acids ◽  
Body Condition ◽  
Condensed Tannins ◽  
Condensed Tannin ◽  
Essential Amino Acids ◽  
Salivary Flow ◽  
Adaptation Period ◽  
Beneficial Effects ◽  
Low Concentrations ◽  
Body Condition Scores

Background: In ruminants, the weaning period is a critical phase, because these young animals are allocated to various facilities and receive different feeds. Alternatively, supplements and additives can be used. The tannins are polyphenols produced by plants; constitute a group of complex secondary metabolic factors that inhibit the consumption by herbivores or the development of microbial agents. The objective of this study was to determine whether the addition of condensed tannin extract from quebracho trees (Schinopsis lorentzii) in lamb diets after weaning would have beneficial effects on growth and health.Materials, Methods & Results: Forty lambs were assigned randomly to 1 of 4 treatments (10 lambs/treatment): T1 (0%; control), T2, T3, and T4 was 0.3, 0.6 and 0.9 % of quebracho extract in the diet, respectively. The T2 (0.3%) lambs had greater body condition scores (P = 0.005), and although they did not differ significantly, they had numerically greater body weight (P = 0.067) and weight gain (P = 0.072), than the control lambs. The anthelmintic and anticoccidial effect was not possible to verify because no animals in any group were naturally infected by helminths or coccidia. The T4 lambs had greater numbers of erythrocytes (days 10 and 40) [P < 0.05], greater hemoglobin concentrations (days 10 and 40), and greater numbers of total leukocytes (day 10) as a result of greater numbers of neutrophil at the end of the adaptation period (day 10) compared to T1. The T3 and T4 lambs had greater (P < 0.05) serum total protein (days 10 and 40) because of greater concentration of serum globulins (days 10 and 40) compared to T1, suggesting that the addition of tannin stimulated the immune system. The addition of quebracho in the diet affected serum concentrations of glucose, triglycerides, and cholesterol at some times and treatments (P < 0.05). However, these changes did not follow a pattern; this finding only suggested that there was an influence on the metabolism of carbohydrates and lipids. The T2 lambs showed no differences on hemogram or serum clinic biochemistries (P > 0.05); however, they had a greater body condition scores than T1 (P < 0.05).Discussion: According to literature, if condensed tannins are supplied in moderate quantities (≤ 5% of diet) to ruminants, they can prevent bloating, increase the supply of undegraded protein in the rumen and improve the use of essential amino acids. It is also known that tannins in diet may increase urea recycling efficiency by increasing the concentration of urea in saliva and salivary flow into the rumen. Studies observed that cattle and sheep given feed containing condensed tannins had higher production of wool, meat, milk, and ovulation rates in females, desirable effects on animal production. According to the literature, dietary supplementation with tannins from chestnut and quebracho (2 g/kg of feed) to Holstein cattle changed the composition of the ruminal microbiota, particularly the bacteria that degrade fiber and starch, reducing the abundance of Prevotella and Fibrobacter, favoring Ruminococcaceae and other members of the phylum Firmicutes, increasing the pH and decreasing urease activity in ruminal fluid. Therefore, when tannins are supplemented at low concentrations, they can stimulate the intake and utilization of essential amino acids in the small intestine, increasing performance and exerting an anthelmintic effect. Our study found that the addition of 0.3% of quebracho in the diet improved lamb growth and health after weaning. It is therefore an additive that can be used to feed lambs in critical post weaning period.

Condensed tannins and the nutritive value of herbage

1990 ◽  
pp. 171-176 ◽  
Author(s):  
G.C. Waghorn ◽  
W.T. Jones ◽  
I.D. Shelton ◽  
W.C. Mcnabb
Keyword(s):  
Amino Acids ◽  
White Clover ◽  
Condensed Tannins ◽  
Dry Matter ◽  
Nutritive Value ◽  
Protein Solubility ◽  
Essential Amino Acids ◽  
Rumen Bacteria ◽  
Plant Proteins ◽  
Grazed Pasture

Many plant species contain condensed tannins (CT), but lotus is one of the few herbages of agricultural importance to contain CT. Lotus has a high nutritive value (NV) despite a moderate protein content and nitrogen digestibility, and this can be explained by the activity of CT during digestion. CT binds plant proteins in the rumen, reducing their solubility and degradation by rumen bacteria. Condensed tannins increase the passage of plant protein to the intestine, and have increased the availability and absorption of essential amino acids by 60% compared with equivalent CT-free forage. Condensed tannins prevent bloat. Dietary concentrations as low as 0.17% CT in the dry matter (DM) can affect protein solubility in the rumen, but concentrations up to about 2-3% of dietary DM are probably optimal for maximising NV. Values exceeding 5.5% of DM inhibit microbial activity excessively and depress voluntary intakes. Ruminant production of milk, meat and wool could be increased by l0-15 % if grazed pasture contained 2-3%CT. These levels would be achieved if white clover could be engineered to contain 7-8% CT in its foliage. Keywords condensed tannins, lotus, nutritive value, herbage, ruminant digestion

scholarly journals Foliar condensed tannin levels in lotus species growing on limed and unlimed soils in South-Eastern Australia

1990 ◽  
pp. 51-54
Author(s):  
W.M. Kelman ◽  
G.J. Tanner
Keyword(s):  
Condensed Tannins ◽  
Condensed Tannin ◽  
Lotus Corniculatus ◽  
Genotypic Differences ◽  
Tannin Content ◽  
Eastern Australia ◽  
Tannin Concentration ◽  
Total Variability ◽  
The Mean ◽  
Agronomic Features

Condensed tannin concentrations (% of dryweight) were measured in leaves of 22 accessions of Lotus corniculatus, 10 accessions of L. pedunculatus, 2 accessions of L. tenuis, and 1 accession of L. subbiflorus from plants growing on limed (pH = 5.2) and unlimed (pH = 4.3) soils near Canberra, A.C.T. There was a non-significant decrease in the mean condensed tannin concentration due to liming. The differences between species and between accessions within species were significant. For the L. corniculatus entries the range of tannin concentrations over the limed and unlimed sites was 0.15-7.28%, with a mean of 2.09%. The highest concentration was found in the otherwise promising accession CPI 66414. L. pedunculatus accessions ranged from 2.53-10.72% in tannin content, with a mean of 5.99%. Two Portuguese accessions that have promising agronomic features for coastal east Australian pastures had unacceptably high tannin levels above 7.50%. Genotypic differences accounted for a large proportion of the total variability for tannin production, so there are good prospects for the improvement of these species by selection and breeding. Keywords: Lotus corniculatus, L. pedunculatus, L. tenuis, L. subbiflorus, condensed tannins

CHICK PERFORMANCE AS AFFECTED BY AUTOCLAVE TREATMENT OF TANNIN-CONTAINING AND TANNIN-FREE CULTIVARS OF FABABEANS

1979 ◽  
Vol 59 (4) ◽  
pp. 781-789 ◽  
Author(s):  
R. R. MARQUARDT ◽  
A. T. WARD
Keyword(s):  
Amino Acids ◽  
Condensed Tannins ◽  
Dry Matter ◽  
Condensed Tannin ◽  
Correlation Coefficients ◽  
Nutrient Utilization ◽  
Broiler Chicks ◽  
Growth Depression ◽  
Autoclave Treatment ◽  
Total Amino Acids

Two experiments were conducted to determine the influence of condensed tannin and autoclave treatment of fababeans on nutrient utilizatiaon by chicks. In the first experiment broiler chicks were fed a series of diets containing five different cultivars of raw or autoclaved fababeans. Three of the cultivars (Triple White, Kodrim and Fidrim) were tannin-free and two (Diana and Hertz-Freya) were tannin-containing. The retention of nutrients in all cases was greater for the birds fed the tannin-free as compared to those fed the tannin-containing cultivars or for those fed the autoclaved as compared to those fed the raw fababeans. The respective retention values for birds fed tannin-containing and tannin-free raw fababeans were 42.3 and 47.9% for dry matter and 73.2 and 83.7% for total amino acids. The corresponding retention values for birds fed raw versus autoclaved fababeans that were free of condensed tannins were 47.9 and 58.7% for dry matter and 83.7 and 90.7% for total amino acids, respectively. The results of these studies suggest that autoclave treatment of fababeans affects fababean utilization by destruction of condensed tannins which accounts for approximately one-half of the total growth depression and by an effect on some other component in fababeans. The second experiment was designed to compare nutrient utilization in chicks fed diets that contained five levels of condensed tannins that had been isolated from fababean testae. The respective correlation coefficients (r) between percent condensed tannins added to the diet and chick performance were: −0.70 for feed intake, −0.90 for weight gain, +0.97 for feed:gain ratio, −0.98 for dry matter retention, −0.99 for protein retention, +0.96 for fat retention, −0.81 for ash retention and −0.72 for calcium retention. It may be concluded that the elimination of condensed tannins from fababeans by genetic selection would significantly improve its nutritional value.

DIGESTION OF NUTRIENTS IN THE SMALL INTESTINE OF PIGS FED DIETS CONTAINING RAW AND AUTOCLAVED FABA BEANS

1976 ◽  
Vol 56 (3) ◽  
pp. 451-456 ◽  
Author(s):  
M. IVAN ◽  
J. P. BOWLAND
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Beneficial Effect ◽  
Dietary Protein ◽  
Dry Matter ◽  
Latin Square ◽  
Sole Source ◽  
Intestinal Uptake ◽  
Gross Energy ◽  
Faba Beans

Four castrated pigs, each fitted with a re-entrant cannula in the terminal ileum, were used to study digestion in the small intestine. A nitrogen-free diet was used for the estimation of metabolic nitrogen and amino acids. Faba beans, as the sole source of dietary protein, were used raw or after autoclaving for 30 or 60 min. The four diets were fed to the pigs in a 4 × 4 latin square experiment. The pigs were fed each diet for 6 days prior to a 24-hr collection of total ileal contents. Autoclaving of faba beans had no significant effect on digestibility of dry matter, gross energy, nitrogen and individual amino acids except arginine, which was significantly increased. The intestinal uptake of arginine was the highest and of cystine the lowest in all faba bean diets. It was concluded that autoclaving faba beans had no beneficial effect on the digestion of nutrients in the small intestine of the pig.

THE ROLE OF CONDENSED TANNINS IN THE DIGESTION OF FRESH Lotus pedunculatus BY SHEEP

1984 ◽  
Vol 64 (5) ◽  
pp. 181-182 ◽  
Author(s):  
T. N. BARRY
Keyword(s):  
Growth Hormone ◽  
Condensed Tannins ◽  
Condensed Tannin ◽  
Soluble Carbohydrate ◽  
Hormone Concentration ◽  
Plasma Growth Hormone ◽  
Tannin Concentration ◽  
Lotus Pedunculatus ◽  
Ruminal Digestion

Relative to non-tannin-containing fresh forages, condensed tannins in Lotus pedunculatus increased duodenal N flow and calculated absorption of amino acids from the small intestine, but depressed ruminal digestion of soluble carbohydrate and hemicellulose. Plasma growth hormone concentration was positively and linearly related to forage condensed tannin concentration. Key words: Condensed tannin, nitrogen digestion, growth hormone

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