scholarly journals The activity of pepsin, chymotrypsin and trypsin during 24 h periods in the small intestine of growing pigs

1982 ◽  
Vol 48 (1) ◽  
pp. 147-159 ◽  
Author(s):  
A. G. Low
Keyword(s):  
Small Intestine ◽  
Fish Meal ◽  
Live Weight ◽  
Growing Pigs ◽  
Casein Diet ◽  
Endogenous Protein ◽  
The Mean ◽  
Maize Oil

1. Digesta were collected from twenty-two pigs, of 40 kg mean live weight, and fitted with single re-entrant cannulas in either the duodenum, jejunum or ileum.2. Three approximately isonitrogenous diets were given to the pigs; their main constituents were: barley, fine wheat offal and white fish meal (diet BWF); starch, sucrose, maize oil, cellulose and either groundnut (diet SSG) or casein (diet SSC).3. The activities of pepsin, chymotrypsin and trypsin were measured every hour in duodenal digesta during 24 h collection periods. Chymotrypsin and trypsin were also measured every hour in jejunal digesta and every 6 h in ileal digesta, during 24 h collection periods.4. The mean total pepsin activities in the duodenal digesta during 24 h collection periods (units for a 40 kg pig given 1·7 kg diet) were: 7764400 (diet BWF), 6078400 (diet SSG), 5801 600 (diet SSC).5. The mean total chymotrypsin activities (units for a 40 kg pig given 1·7 kg diet) in digesta in the duodenum, jejunum and ileum respectively were: 62920, 59560, 21 880 (diet BWF), 78240, 68400, 24680 (diet SSG). 75280, 76120, 6160 (diet SSC).6. The mean total trypsin activities (units for a 40 kg pig given 1·7 kg diet) in digesta from the duodenum, jejunum and ileum, respectively were: 256840, 362840, 77600 (diet BWF), 211 200, 205280. 46720 (diet SSG) 325720, 428560, 13600 (diet SSC).7. It was calculated that the total weights of pepsin, chymotrypsin and trypsin in duodenal digesta in 24 h periods were between 6·2 and 7·1 g. This represents 20–25% of previously published estimates of the amounts of endogenous protein in this part of the gut.

Studies on digestion and absorption in the intestines of growing pigs

1978 ◽  
Vol 39 (3) ◽  
pp. 515-526 ◽  
Author(s):  
A. G. Low ◽  
I. G. Partridge ◽  
I. E. Sambrook
Keyword(s):  
Dry Matter ◽  
Fish Meal ◽  
Live Weight ◽  
Growing Pigs ◽  
The Other ◽  
Casein Diet ◽  
Total Flow ◽  
Flow Rates ◽  
Maize Oil

1. Digesta were collected from twenty-three pigs, initially of 30 kg live weight, and fitted with single Ash re-entrant cannulas in either the duodenum, jejunum or ileum. A further twenty-four pigs were used in a conventional digestibility trial.2. The diets contained: barley, fine wheat offal, white fish meal, minerals and vitamins (diet BWF); starch, sucrose, maize oil, cellulose, minerals, vitamins and either groundnut (diet SSG) or casein (diet SSC).3. The flow-rates of dry matter (dm), ash and water were measured hourly in the duodenum and jejunum, and every 6 h in the ileum during 24 h collection periods. Faeces were collected during 5 d periods.4. Marked increases in the flow rates of each of the digesta components after feeding each diet were observed in the duodenum and jejunum, but not in the ileum. The total flow in 24 h periods was much lower in the ileum than at the other sites.5. Values for the ratio, dm output:intake for dm outputs from the duodenal, jejunal or ileal cannulas, and in faeces, in 24 h periods were respectively 0.94, 0.81, 0.28 and 0.22 for diet BWF; 0.96, 0.76, 0.20 and 0.15 for diet SSG; 0.96, 0.73, 0.08 and 0.04 for diet SSC. The corresponding values for ash were: 1.47, 1.65, 0.74 and 0.53 for diet BWF; 1.28, 1.34, 0.59 and 0.51 for diet SSG; 1.63, 1.35, 0.50 and 0.26 for diet SSC. The corresponding values for water were: 3.49, 3.41, 0.88 and 0.18 for diet BWF, 2.75, 2.80, 0.80 and 0.08 for diet SSG and 2.61, 1.87, 0.23 and 0.01 for diet SSC.

scholarly journals Studies on digestion and absorption in the intestines of growing pigs

1979 ◽  
Vol 41 (1) ◽  
pp. 137-146 ◽  
Author(s):  
A. G. Low
Keyword(s):  
Fish Meal ◽  
Liquid Fraction ◽  
Live Weight ◽  
Growing Pigs ◽  
Casein Diet ◽  
Flow Rates ◽  
Maize Oil

1. Digesta were collected from twenty-three pigs, initially of 30 kg live weight, and fitted with single Ash re-entrant cannulas in either the duodenum, jejunum or ileum. A further twenty-four pigs were used in a conventional digestibility trial.2. Three approximately isonitrogenous diets were used; they contained: barley, fine wheat offal, white fish meal, minerals and vitamins (diet BWF), starch, sucrose, maize oil, cellulose, minerals, vitamins and either groundnut (diet SSG) or casein (diet SSC).3. The flow-rates of nitrogen (N), liquid-fraction N (LN) and non-protein liquid-fraction N (NPLN) were measured hourly in the duodenum and jejunum and every 6 h in the ileum during 24 h collection periods. Faeces were collected during 5 d periods.4. Marked increases in flow of N, LN and NPLN were seen in the duodenum and jejunum after feeding each diet, but not in the ileum.5. Values for, N output: intake from the duodenal, jejunal or ideal cannulas and in faeces in 24 h periods were respectively: 0.98, 0.88, 0.25 and 0.21 for diet BWF; 1.00, 0.97, 0.22 and 0.24 for diet SSG; 0.98, 0.73, 0.09 and 0.03 for diet SSC. The corresponding values for LN in the duodenum, jejunum and ileum respectively were: 0.53, 0.53 and 0.12 for diet BWF; 0.46, 0.60 and 0.13 for diet SSG; 0.57, 0.50 and 0.06 for diet SSC. The corresponding values for NPLN in the duodenum, jejunum and ileum respectively were: 0.41, 0.38 and 0.09 for diet BWF; 0.35, 0.36 and 0.08 for diet SSG; 0.46, 0.38 and 0.04 for diet SSC.6. The results are discussed in relation to similar studies, particularly in pigs with duodenal cannulas, in which widely different observations were made.

scholarly journals Studies on digestion and absorption in the intestines of growing pigs

1979 ◽  
Vol 42 (2) ◽  
pp. 267-277 ◽  
Author(s):  
I. E. Sambrook
Keyword(s):  
Small Intestine ◽  
Fish Meal ◽  
Growing Pigs ◽  
Casein Diet ◽  
Total Carbohydrate ◽  
Rate Of Absorption ◽  
Groundnut Meal ◽  
Reducing Substances ◽  
Site Of Absorption ◽  
Maize Oil

1. Seventeen pigs were fitted with single re-entrant cannulas in either the duodenum (posterior to the entry of the bile and pancreativ ducts), the mid-jejunum, or the terminal ileum. A further twenty-four pigs were used in a conventional digestibility trial.2. Three diets were used: these contained barley, fine wheat offal, white fish meal, minerals and vitamins (diet BWF); starch, sucrose, maize oil, cellulose, minerals, vitamins and either groundnut meal (diet SSG) or casein (diet SSC).3. The quantities of total carbohydrate (TC), total reducing substances (TRS) and glucose (G) passing through the re-entrant cannulas and excreted in the faeces in 24 h were measured. These were used to determine the net absorption of the carbohydrate fractions in the different regions of the intestine.4. The small intestine was the principal site of absorption of TC, TRS and G, but there were differences between the diets in the quantities of each of these carbohydrate fractions that were absorbed in the different regions of the small intestine studied.5. The quantities of TRS and G in solution were very low for all diets at all sites, indicating that the rate of absorption of the products of hydrolysis kept pace with their rate of formation.

Studies on digestion and absorption in the intestines of growing pigs

1978 ◽  
Vol 39 (3) ◽  
pp. 527-537 ◽  
Author(s):  
I. G. Partridge
Keyword(s):  
Small Intestine ◽  
Large Intestine ◽  
Fish Meal ◽  
Live Weight ◽  
Growing Pigs ◽  
Pancreatic Ducts ◽  
Calcium Phosphorus ◽  
Five Elements ◽  
Sodium And Potassium ◽  
P Absorption

1. Pigs growing from 20 to 60 kg live weight were given diets based on barley, weatings and fish meal, or starch, sucrose and groundnut meal or starch, sucrose and casein. Seventeen pigs were fitted with single re-entrant cannulas in the duodenum (posterior to entry of bile and pancreatic ducts), jejunum or terminal ileum and twenty-four non-cannulated pigs were used in a conventional digestibility trial.2. The amounts of calcium, phosphorus, magnesium, sodium and potassium passing through the reentrant cannulas and amounts excreted in the faeces were measured. These values were used to calculate the direction and extent of net movements of the five elements through the walls of the four parts of the digestive tract anterior to the collection sites.3. The small intestine was the principal site of Ca and P absorption but there were differences between the diets in the relative importance of the regions anterior and posterior to the mid-jejunum.4. Secretion of small amounts of Mg occurred in the anterior small intestine; the ileum and large intestine were the principal sites of net absorption.5. There was a large net secretion of Na anterior to the duodenal cannulas and further secretion into the anterior small intestine with each diet. There were marked differences between diets in the amounts secreted but the ileal Na concentration was the same in each instance. Absorption occurred in the ileum and large intestine.6. Secretion of small amounts of K was evident anterior to the duodenal cannulas and net absorption occurred in both parts of the small intestine with each diet.

scholarly journals Studies on digestion and absorption in the intestines of growing pigs

1979 ◽  
Vol 41 (1) ◽  
pp. 147-156 ◽  
Author(s):  
A. G. Low
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Large Intestine ◽  
Fish Meal ◽  
Live Weight ◽  
Growing Pigs ◽  
Casein Diet ◽  
Total Flow ◽  
Dietary Amino Acids

1. Digesta were collected from seventeen pigs initially of 30 kg live weight fitted with single re-entrant cannulas in either the duodenum, jejunum or ileum. A further twenty-four pigs were used in a conventional digestibility trial.2. The pigs received three types of diet containing: barley, fine wheat offal, white fish meal, minerals and vitamins (diet BWF); starch, sucrose, maize oil, cellulose, minerals and vitamins and either groundnut (diet SSG) or casein (diet SSC).3. Amino acids were measured in samples representative of the digesta flow in 24 h periods and in the faeces collected in 5 d periods.4. For each diet the total flow in 24 h periods in the duodenum for aspartic acid, threonine, serine and glycine exceeded or equalled intake, while the amounts of the other amino acids were usually rather less than intake.5. For each diet in the jejunum, the amounts of glycine and cystine exceeded intake in 24 h periods, while methionine, arginine and tyrosine were the most rapidly absorbed amino acids anterior to the cannula site. On average 0.22, 0.25 and 0.31 of the dietary amino acids were absorbed anterior to the cannula site for diets BWF, SSG and SSC, respectively.6. For each diet in the ileum, the least apparently absorbed dietary amino acids were glycine and cystine. On average 0.81, 0.83 and 0.95 of the dietary amino acids were absorbed anterior to the cannula site for diets BWF, SSG and SSC, respectively.7. There was net disappearance of most amino acids in the large intestine, but some net accumulation occurred in this region.8. The results are discussed in relation to the amino acid composition of endogenous secretions (particularly glycine in bile), protease and peptidase specificity, free amino acid absorption and the role of the microflora in the large intestine.

THE EFFECT OF INGESTED CHLORTETRACYCLINE ON SOME HYDROLASES AND ORGANS ASSOCIATED WITH THE DIGESTIVE PROCESS IN GROWING PIGS: II. PROTEASE, AMYLASE, AND GELLULASE ACTIVITY OF THE CONTENTS OF THE SMALL INTESTINE AND OF THE CECUM; WEIGHTS OF SMALL INTESTINE AND MUCOSA

1957 ◽  
Vol 35 (3) ◽  
pp. 195-202 ◽  
Author(s):  
M. A. A. Vonk ◽  
L. W. McElroy ◽  
R. T. Berg
Keyword(s):  
Small Intestine ◽  
Dry Matter ◽  
Cellulase Activity ◽  
Dry Weight ◽  
Growing Pigs ◽  
Weanling Pigs ◽  
Intestinal Contents ◽  
Wet Weight ◽  
The Mean ◽  
Ad Libitum

Four treatments, involving differences in method of feeding and interval between last feed and slaughter, were employed in a study of the effect of dietary chlortetracycline on protease, amylase, and cellulase activity in the intestinal and cecal contents of 16 pairs of weanling pigs. Most consistent results were obtained with six pairs which were limited pair-fed except for the final feeding during which feed was available ad libitum for a 4-hour period ending 18 hours before slaughter. The mean total activities of all three hydrolases in the contents of the small intestines and of the ceca of the antibiotic-fed animals of these six pairs were significantly greater than in those of the control animals. Expressed as activity per gram dry matter of intestinal contents, significantly higher values for protease and amylase, but not for cellulase, were observed in the pigs that had received chlortetracycline. When the combined results obtained from all 16 pairs of the experimental animals were analyzed, the results showed that on a basis of activity per gram dry matter of intestinal contents, ingested chlortetracycline was associated with significant increases in amylase and cellulase but not in protease activity. Protease, amylase, and cellulase activities per gram dry matter of cecal contents were higher for pigs fed the antibiotic than for their controls. The mean wet weight of the empty small intestine and the mean dry weight of the mucosa scraped from the anterior 3-meter section of the small intestine were lower for the chlortetracycline-fed animals, but the differences were not statistically significant.

scholarly journals Effect of guar gum on gastric emptying in growing pigs

1986 ◽  
Vol 55 (1) ◽  
pp. 87-98 ◽  
Author(s):  
Anna L. Rainbird ◽  
A. G. Low
Keyword(s):  
Gastric Emptying ◽  
Dry Matter ◽  
Guar Gum ◽  
Control Diet ◽  
Live Weight ◽  
Growing Pigs ◽  
Postprandial Blood Glucose ◽  
The Mean ◽  
Fat Diets ◽  
Blood Glucose Concentrations

1. Simple gastric cannulas were surgically fitted to four pigs, initially of 30 kg live weight, to examine the effects of guar gum on gastric emptying.2. Four semi-purified high-fat diets based on starch, casein, soya-bean oil and tallow were given to each pig. They contained 0 (control), 20, 40 or 60 g powdered guar gum/kg diet. The meals as fed contained 257 g dry matter (DM)/kg.3. The contents of the stomach were evacuated, with rinsing, before feeding or 0.5, 1, 2 or 4 h after feeding.4. The mean pH of the digesta was unaffected by guar gum until 4 h after feeding when the value increased as the amount of guar gum in the diet rose.5. The only significant effects of guar gum on the emptying of digesta and its components (compared with the control diet) were to reduce the rate of emptying of (a) digesta 1 h after feeding (60 g/kg diet) and 4 h after feeding (40 and 60 g/kg diets), (b) dry matter and glucose 1 h after feeding (60 g/kg diet), (c) nitrogen 1 h after feeding (60 g/kg diet) and 4 h after feeding (40 and 60 g/kg diets).6. When expressed on a half-time (T50) basis, the emptying of digesta and N (but not of DM and glucose) were significantly slower for diets containing 40 and 60 g guar gum/kg than for the control diet.7. The apparent viscosity of the gastric digesta ranged between 0.5 and 23.7% of the values for the diets as consumed.8. It was concluded that the effects of guar gum on gastric emptying of high-solid meals were small, and that this was unlikely to be an important aspect of the mechanism by which guar gum reduces postprandial blood glucose concentrations.

Studies on digestion and absorption in the intestines of growing pigs

1978 ◽  
Vol 39 (3) ◽  
pp. 539-545 ◽  
Author(s):  
I. G. Partridge
Keyword(s):  
Small Intestine ◽  
Water Content ◽  
Terminal Ileum ◽  
Live Weight ◽  
Growing Pigs ◽  
Concomitant Reduction ◽  
Calcium Phosphorus ◽  
High Cellulose ◽  
Immediate Response

1. Seven pigs of 30 kg initial live weight were fitted with re-entrant cannulas in the terminal ileum. Each was fed, in succession, four purified diets having cellulose and sodium levels (g/kg) of 30 and 2.7, 30 and 0.9, 90 and 2.7 or 90 and 0.9, respectively. Collections of digesta (24 h) and 3 or 4 d collections of faeces were made.2. There was a greater throughput of ileal digesta with the high-cellulose diets than with the low-cellulose diets, mainly due to increased water content, and there was a concomitant reduction in the net absorption of Na from the small intestine. The immediate response to reduced Na intake was increased secretion of Na into the gut lumen anterior to the terminal ileum; this was more pronounced with the high-cellulose diet. Na concentrations in ileal digesta were very similar for all four diets.3. The apparent absorptions of calcium, phosphorus, magnesium, potassium and zinc were reduced by the high-cellulose diets. In each instance this was due to reduced absorption posterior to the terminal ileum.

A note on the effect of sudden changes in the composition of the diet of growing pigs

1973 ◽  
Vol 16 (3) ◽  
pp. 315-317
Author(s):  
R. M. Livingstone
Keyword(s):  
Crude Protein ◽  
Fish Meal ◽  
Live Weight ◽  
Growing Pigs ◽  
The Other ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Digestible Energy ◽  
Per Se

SUMMARYTwo diets, one based on barley and white-fish meal and the other on oats, wheat and meat and bone meal, with similar concentrations of digestible energy, crude protein, lysine and methionine+cystine were used to investigate the effects of sudden changes in the composition of the diet on the performance of pigs growing from 32 kg live weight, over a period of 77 days.The diets were given separately, alternated weekly with sudden changes from one diet to the other, or as a 1:1 mixture.Changes in diet per se had insignificant effects on performance.

scholarly journals Studies on digestion and absorption in the intestines of growing pigs

1979 ◽  
Vol 42 (2) ◽  
pp. 279-287 ◽  
Author(s):  
I. E. Sambrook
Keyword(s):  
Volatile Fatty Acids ◽  
Fish Meal ◽  
Distal Portion ◽  
Growing Pigs ◽  
Pancreatic Ducts ◽  
Casein Diet ◽  
Point Of Entry ◽  
Proximal Small Intestine ◽  
Acid Detergent Fibre ◽  
Groundnut Meal

1. Seventeen pigs fitted with single re-entrant cannulas in either the duodenum (posterior to the point of entry of the bile and pancreatic ducts), the mid-jejunum, or the terminal ileum, and a further twenty-four non-cannulated pigs were used.2. Three diets were used: these contained barley, fine wheat offal, white fish meal, minerals and vitamins (diet BWF); starch, sucrose, maize oil, cellulose, minerals, vitamins and either groundnut meal (diet SSG) or casein (diet SSC).3. The quantities of total lipid (TL), acid-detergent fibre (ADF) and volatile fatty acids (VFA) passing through the re-entrant cannulas and excreted in the faeces in 24 h were measured. These were used to determine the net absorption (or synthesis or secretion) in the different regions of the intestine.4. There was substantial secretion of TL into the proximal small intestine and major absorption from the distal portion for all three diets. In the large intestine there was little or no further net absorption for diets SSG and SSC, but significant synthesis or secretion for diet BWF.5. Fibre digestion occurred in both the small and large intestines and the apparent absorption of ADF in the different regions studied was influenced by diet.6. There were detectable amounts of VFA at all sites but only at the ileum and in the faeces could they be accurately measured. Diet did not affect the molar proportions of acetate, propionate and butyrate in ileal digesta, but did have an effect on these VFA in the faeces.

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